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Strain Name:
C57BL/6J-MtgxR9127Btlr/Mmmh
Stock Number:
068926-MU
Citation ID:
RRID:MMRRC_068926-MU
Other Names:
R9127 (G1)
Major Collection:

Strain Information

Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: beta fodrin, Spnb-2, spectrin G, brain spectrin, elf3, elf1, 9930031C03Rik, non-erythrocytic, Spnb2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20742
Homologene: 2354
Rnd3
Name: Rho family GTPase 3
Synonyms: 2610017M01Rik, Arhe, Rhoe
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74194
HGNC: HGNC:671
Homologene: 21074
Rasgrp2
Name: RAS, guanyl releasing protein 2
Synonyms: CalDAG-GEFI, Caldaggef1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19395
HGNC: HGNC:9879
Homologene: 4250
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Chtf8
Name: CTF8, chromosome transmission fidelity factor 8
Synonyms: 5830457O10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 214987
Homologene: 84588
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 6,262,849 bp
  • A to T, chromosome 1 at 74,919,351 bp
  • A to C, chromosome 1 at 127,366,460 bp
  • T to C, chromosome 1 at 159,886,110 bp
  • A to G, chromosome 1 at 171,338,841 bp
  • A to T, chromosome 1 at 173,695,834 bp
  • T to C, chromosome 1 at 191,907,230 bp
  • G to A, chromosome 1 at 192,901,823 bp
  • T to C, chromosome 2 at 3,438,088 bp
  • C to A, chromosome 2 at 32,213,752 bp
  • A to G, chromosome 2 at 32,306,067 bp
  • A to T, chromosome 2 at 51,132,401 bp
  • G to A, chromosome 2 at 89,326,130 bp
  • C to T, chromosome 2 at 121,327,644 bp
  • A to T, chromosome 2 at 125,382,065 bp
  • T to A, chromosome 2 at 129,304,795 bp
  • C to T, chromosome 2 at 155,970,122 bp
  • T to C, chromosome 3 at 88,762,336 bp
  • G to T, chromosome 3 at 104,804,343 bp
  • A to T, chromosome 4 at 14,593,873 bp
  • A to T, chromosome 4 at 66,403,927 bp
  • A to G, chromosome 4 at 86,289,790 bp
  • G to A, chromosome 4 at 123,246,092 bp
  • A to T, chromosome 4 at 126,507,111 bp
  • A to T, chromosome 5 at 8,674,707 bp
  • A to T, chromosome 5 at 15,031,099 bp
  • G to A, chromosome 5 at 24,476,441 bp
  • A to G, chromosome 5 at 95,803,764 bp
  • G to T, chromosome 5 at 115,936,837 bp
  • G to A, chromosome 5 at 144,831,020 bp
  • T to A, chromosome 6 at 70,883,720 bp
  • T to A, chromosome 6 at 91,233,491 bp
  • T to A, chromosome 6 at 123,139,259 bp
  • T to G, chromosome 6 at 123,235,488 bp
  • T to A, chromosome 7 at 45,280,250 bp
  • T to C, chromosome 7 at 45,979,760 bp
  • T to A, chromosome 7 at 101,992,662 bp
  • A to C, chromosome 7 at 112,271,382 bp
  • A to T, chromosome 7 at 126,498,221 bp
  • T to C, chromosome 8 at 16,223,272 bp
  • T to G, chromosome 8 at 45,962,366 bp
  • C to T, chromosome 8 at 72,733,406 bp
  • A to G, chromosome 8 at 106,887,008 bp
  • A to G, chromosome 9 at 3,634,553 bp
  • A to G, chromosome 9 at 19,338,730 bp
  • A to G, chromosome 9 at 42,974,574 bp
  • T to A, chromosome 9 at 51,144,715 bp
  • A to T, chromosome 9 at 58,220,377 bp
  • T to A, chromosome 9 at 103,068,044 bp
  • A to T, chromosome 9 at 108,208,535 bp
  • T to C, chromosome 10 at 18,011,696 bp
  • G to T, chromosome 10 at 25,279,778 bp
  • A to T, chromosome 10 at 82,622,754 bp
  • T to C, chromosome 10 at 128,632,618 bp
  • T to G, chromosome 11 at 9,292,080 bp
  • A to G, chromosome 11 at 30,154,356 bp
  • T to A, chromosome 11 at 48,888,773 bp
  • A to T, chromosome 11 at 73,928,341 bp
  • T to A, chromosome 11 at 83,504,259 bp
  • T to A, chromosome 11 at 104,850,581 bp
  • C to T, chromosome 12 at 110,793,163 bp
  • A to T, chromosome 12 at 114,487,259 bp
  • T to C, chromosome 13 at 13,634,242 bp
  • T to C, chromosome 13 at 35,968,638 bp
  • T to C, chromosome 13 at 49,661,999 bp
  • C to T, chromosome 13 at 58,576,767 bp
  • A to G, chromosome 13 at 59,743,014 bp
  • A to T, chromosome 13 at 61,567,421 bp
  • T to C, chromosome 13 at 91,859,218 bp
  • C to A, chromosome 14 at 21,987,350 bp
  • G to A, chromosome 15 at 86,091,324 bp
  • C to A, chromosome 15 at 89,355,568 bp
  • T to C, chromosome 15 at 96,414,883 bp
  • A to T, chromosome 16 at 4,547,319 bp
  • A to G, chromosome 17 at 12,739,351 bp
  • G to A, chromosome 17 at 26,962,121 bp
  • G to T, chromosome 17 at 28,286,375 bp
  • T to A, chromosome 17 at 50,611,004 bp
  • A to G, chromosome 18 at 37,449,038 bp
  • G to A, chromosome 18 at 84,680,996 bp
  • A to T, chromosome 18 at 89,269,031 bp
  • A to T, chromosome 19 at 6,404,408 bp
  • A to G, chromosome 19 at 13,165,032 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9127 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068926-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.