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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR9184Btlr/Mmmh
Stock Number:
068979-MU
Citation ID:
RRID:MMRRC_068979-MU
Other Names:
R9184 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Prkaca
Name: protein kinase, cAMP dependent, catalytic, alpha
Synonyms: Cs, C alpha, PKA, Pkaca
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18747
HGNC: HGNC:9380
Homologene: 121574

Additional Resources
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ClinGen
Arc
Name: activity regulated cytoskeletal-associated protein
Synonyms: Arc3.1, arg 3.1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11838
HGNC: HGNC:648
Homologene: 9056

Additional Resources
IMPC
Google
Entrez
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Neurod4
Name: neurogenic differentiation 4
Synonyms: MATH-3, Math3, Atoh3, bHLHa4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11923
VEGA: 10
Homologene: 7234

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
Monarch
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Gad2
Name: glutamic acid decarboxylase 2
Synonyms: GAD65, Gad-2, 6330404F12Rik, GAD(65)
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14417
HGNC: HGNC:4093
Homologene: 20223

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 8,677,832 bp
  • T to A, chromosome 1 at 87,440,589 bp
  • T to A, chromosome 1 at 118,543,178 bp
  • A to T, chromosome 1 at 150,104,424 bp
  • T to A, chromosome 1 at 180,144,736 bp
  • G to A, chromosome 2 at 22,668,319 bp
  • A to G, chromosome 2 at 31,816,476 bp
  • A to G, chromosome 2 at 52,328,755 bp
  • A to T, chromosome 2 at 76,769,605 bp
  • C to A, chromosome 2 at 88,683,175 bp
  • T to A, chromosome 2 at 89,844,950 bp
  • G to A, chromosome 2 at 146,077,388 bp
  • G to A, chromosome 2 at 163,858,686 bp
  • A to T, chromosome 3 at 38,982,443 bp
  • A to T, chromosome 3 at 107,614,663 bp
  • T to C, chromosome 4 at 11,962,143 bp
  • C to T, chromosome 4 at 14,506,630 bp
  • T to C, chromosome 4 at 24,596,182 bp
  • G to C, chromosome 4 at 47,288,200 bp
  • G to A, chromosome 4 at 118,384,529 bp
  • G to T, chromosome 4 at 138,321,010 bp
  • T to A, chromosome 5 at 21,813,882 bp
  • T to A, chromosome 5 at 150,800,784 bp
  • G to T, chromosome 6 at 34,753,577 bp
  • G to A, chromosome 6 at 92,411,524 bp
  • T to C, chromosome 7 at 6,379,301 bp
  • A to G, chromosome 7 at 43,952,734 bp
  • T to A, chromosome 8 at 24,647,831 bp
  • G to A, chromosome 8 at 83,990,676 bp
  • T to C, chromosome 8 at 106,507,269 bp
  • C to T, chromosome 8 at 124,045,151 bp
  • T to C, chromosome 9 at 18,815,842 bp
  • T to C, chromosome 9 at 44,135,248 bp
  • T to C, chromosome 9 at 79,898,260 bp
  • A to G, chromosome 10 at 80,002,856 bp
  • A to T, chromosome 10 at 130,271,089 bp
  • T to C, chromosome 11 at 43,443,397 bp
  • T to C, chromosome 11 at 43,474,353 bp
  • A to G, chromosome 11 at 71,181,241 bp
  • T to A, chromosome 11 at 74,892,008 bp
  • T to C, chromosome 11 at 78,271,388 bp
  • A to G, chromosome 12 at 112,654,718 bp
  • T to A, chromosome 13 at 9,693,439 bp
  • T to G, chromosome 13 at 33,085,410 bp
  • C to A, chromosome 13 at 119,381,734 bp
  • C to T, chromosome 14 at 16,818,881 bp
  • T to A, chromosome 14 at 16,818,882 bp
  • G to T, chromosome 14 at 63,138,095 bp
  • A to G, chromosome 15 at 28,340,406 bp
  • A to G, chromosome 15 at 74,671,930 bp
  • T to A, chromosome 17 at 15,742,289 bp
  • T to A, chromosome 17 at 22,571,841 bp
  • T to C, chromosome 17 at 34,587,390 bp
  • ACTTCTTCT to ACTTCT, chromosome 17 at 56,571,292 bp
  • T to A, chromosome 17 at 71,233,916 bp
  • C to T, chromosome 18 at 34,949,115 bp
  • A to T, chromosome 18 at 37,687,407 bp
  • A to G, chromosome 18 at 43,582,287 bp
  • T to A, chromosome 18 at 70,509,179 bp
  • T to A, chromosome 18 at 76,289,100 bp
  • T to C, chromosome 19 at 6,241,857 bp
  • T to C, chromosome 19 at 28,531,607 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9184 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068979-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.