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Strain Name:
C57BL/6J-MtgxR9185Btlr/Mmmh
Stock Number:
069033-MU
Citation ID:
RRID:MMRRC_069033-MU
Other Names:
R9185 (G1)
Major Collection:

Strain Information

Cr1l
Name: complement C3b/C4b receptor 1 like
Synonyms: mCRY, Crry
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12946
Homologene: 128275
Ctbp2
Name: C-terminal binding protein 2
Synonyms: D7Ertd45e, Ribeye, Gtrgeo6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13017
HGNC: HGNC:2495
Homologene: 75187
Zfand6
Name: zinc finger, AN1-type domain 6
Synonyms: Awp1, 3110005P07Rik, Za20d3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 65098
Homologene: 10372
Tbce
Name: tubulin-specific chaperone E
Synonyms: 2610206D02Rik, C530005D02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70430
Homologene: 37744
Zic1
Name: zinc finger protein of the cerebellum 1
Synonyms: odd-paired homolog
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22771
Homologene: 2562
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Mier3
Name: MIER family member 3
Synonyms: D130064H19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218613
Homologene: 18196
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 10,144,779 bp (GRCm38)
  • G to A, chromosome 1 at 67,209,672 bp (GRCm38)
  • A to G, chromosome 1 at 162,704,643 bp (GRCm38)
  • G to A, chromosome 1 at 171,216,386 bp (GRCm38)
  • A to G, chromosome 1 at 179,050,289 bp (GRCm38)
  • C to A, chromosome 1 at 195,114,745 bp (GRCm38)
  • T to C, chromosome 2 at 29,876,021 bp (GRCm38)
  • A to C, chromosome 2 at 87,092,856 bp (GRCm38)
  • A to T, chromosome 2 at 87,213,830 bp (GRCm38)
  • T to C, chromosome 2 at 130,563,849 bp (GRCm38)
  • A to G, chromosome 2 at 181,230,090 bp (GRCm38)
  • T to A, chromosome 3 at 97,758,816 bp (GRCm38)
  • T to A, chromosome 3 at 107,628,214 bp (GRCm38)
  • G to C, chromosome 4 at 47,288,200 bp (GRCm38)
  • A to G, chromosome 4 at 63,328,650 bp (GRCm38)
  • T to C, chromosome 4 at 143,655,328 bp (GRCm38)
  • T to G, chromosome 5 at 111,223,476 bp (GRCm38)
  • G to A, chromosome 5 at 117,363,671 bp (GRCm38)
  • T to G, chromosome 5 at 129,847,068 bp (GRCm38)
  • C to T, chromosome 6 at 42,141,165 bp (GRCm38)
  • T to C, chromosome 6 at 55,318,276 bp (GRCm38)
  • T to C, chromosome 6 at 90,413,434 bp (GRCm38)
  • G to T, chromosome 6 at 115,957,565 bp (GRCm38)
  • C to G, chromosome 6 at 122,077,483 bp (GRCm38)
  • T to C, chromosome 6 at 142,459,386 bp (GRCm38)
  • T to A, chromosome 7 at 7,284,198 bp (GRCm38)
  • T to C, chromosome 7 at 24,744,243 bp (GRCm38)
  • A to T, chromosome 7 at 38,099,830 bp (GRCm38)
  • A to G, chromosome 7 at 84,634,350 bp (GRCm38)
  • A to G, chromosome 7 at 92,933,049 bp (GRCm38)
  • C to T, chromosome 7 at 102,724,202 bp (GRCm38)
  • G to A, chromosome 7 at 104,160,888 bp (GRCm38)
  • A to G, chromosome 7 at 119,578,198 bp (GRCm38)
  • A to T, chromosome 7 at 133,013,983 bp (GRCm38)
  • A to G, chromosome 8 at 69,663,223 bp (GRCm38)
  • T to C, chromosome 8 at 84,237,004 bp (GRCm38)
  • A to T, chromosome 8 at 88,665,252 bp (GRCm38)
  • T to C, chromosome 8 at 120,568,169 bp (GRCm38)
  • T to C, chromosome 8 at 123,661,054 bp (GRCm38)
  • A to G, chromosome 9 at 21,038,869 bp (GRCm38)
  • T to A, chromosome 9 at 39,772,108 bp (GRCm38)
  • A to G, chromosome 9 at 44,152,840 bp (GRCm38)
  • G to T, chromosome 9 at 91,364,489 bp (GRCm38)
  • T to A, chromosome 10 at 44,004,095 bp (GRCm38)
  • T to C, chromosome 11 at 49,535,674 bp (GRCm38)
  • A to G, chromosome 11 at 67,695,654 bp (GRCm38)
  • T to C, chromosome 11 at 69,134,288 bp (GRCm38)
  • T to C, chromosome 11 at 69,879,850 bp (GRCm38)
  • T to C, chromosome 11 at 115,880,429 bp (GRCm38)
  • T to C, chromosome 12 at 110,635,503 bp (GRCm38)
  • A to T, chromosome 13 at 13,998,442 bp (GRCm38)
  • T to C, chromosome 13 at 14,317,043 bp (GRCm38)
  • A to G, chromosome 13 at 38,939,982 bp (GRCm38)
  • G to A, chromosome 13 at 42,184,499 bp (GRCm38)
  • A to T, chromosome 13 at 49,191,250 bp (GRCm38)
  • T to C, chromosome 13 at 111,714,726 bp (GRCm38)
  • G to T, chromosome 14 at 20,145,067 bp (GRCm38)
  • A to G, chromosome 14 at 52,463,527 bp (GRCm38)
  • T to C, chromosome 14 at 61,206,666 bp (GRCm38)
  • T to A, chromosome 15 at 44,589,623 bp (GRCm38)
  • T to A, chromosome 15 at 91,343,703 bp (GRCm38)
  • G to A, chromosome 16 at 17,276,438 bp (GRCm38)
  • A to T, chromosome 16 at 92,348,908 bp (GRCm38)
  • A to C, chromosome 17 at 26,963,083 bp (GRCm38)
  • A to T, chromosome 17 at 38,148,863 bp (GRCm38)
  • A to T, chromosome 17 at 40,914,343 bp (GRCm38)
  • G to A, chromosome 17 at 56,661,252 bp (GRCm38)
  • T to C, chromosome 17 at 68,840,638 bp (GRCm38)
  • A to G, chromosome 19 at 8,344,553 bp (GRCm38)
  • A to T, chromosome 19 at 13,633,401 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9185 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069033-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.