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Strain Name:
C57BL/6J-MtgxR9228Btlr/Mmmh
Stock Number:
069060-MU
Citation ID:
RRID:MMRRC_069060-MU
Other Names:
R9228 (G1)
Major Collection:

Strain Information

Sphk2
Name: sphingosine kinase 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56632
Homologene: 32456
Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81897
Homologene: 68126
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Rbms1
Name: RNA binding motif, single stranded interacting protein 1
Synonyms: YC1, MSSP-3, MSSP-2, MSSP-1, 2600014B10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56878
HGNC: HGNC:9907
Homologene: 9640
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Nmral1
Name: NmrA-like family domain containing 1
Synonyms: 1110025F24Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67824
Homologene: 41388
Pmepa1
Name: prostate transmembrane protein, androgen induced 1
Synonyms: N4wbp4, 2210418I02Rik, PMEPA1, STAG1, Tmepai
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65112
Homologene: 10608
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 71,293,440 bp (GRCm38)
  • A to G, chromosome 1 at 72,674,051 bp (GRCm38)
  • A to G, chromosome 1 at 91,438,359 bp (GRCm38)
  • T to C, chromosome 1 at 163,862,097 bp (GRCm38)
  • C to T, chromosome 1 at 167,308,563 bp (GRCm38)
  • T to C, chromosome 1 at 179,784,120 bp (GRCm38)
  • T to A, chromosome 1 at 185,455,194 bp (GRCm38)
  • T to C, chromosome 2 at 11,781,507 bp (GRCm38)
  • G to A, chromosome 2 at 60,779,743 bp (GRCm38)
  • T to C, chromosome 2 at 66,299,755 bp (GRCm38)
  • G to T, chromosome 2 at 69,308,465 bp (GRCm38)
  • T to C, chromosome 2 at 71,522,556 bp (GRCm38)
  • A to G, chromosome 2 at 76,752,848 bp (GRCm38)
  • T to A, chromosome 2 at 87,457,563 bp (GRCm38)
  • A to G, chromosome 2 at 112,242,073 bp (GRCm38)
  • G to A, chromosome 2 at 153,666,060 bp (GRCm38)
  • C to T, chromosome 2 at 155,970,122 bp (GRCm38)
  • A to G, chromosome 2 at 163,338,686 bp (GRCm38)
  • A to G, chromosome 2 at 168,184,878 bp (GRCm38)
  • T to C, chromosome 2 at 173,276,169 bp (GRCm38)
  • G to A, chromosome 3 at 59,336,422 bp (GRCm38)
  • C to T, chromosome 3 at 92,868,644 bp (GRCm38)
  • G to A, chromosome 3 at 122,924,625 bp (GRCm38)
  • A to T, chromosome 4 at 41,116,422 bp (GRCm38)
  • T to C, chromosome 4 at 83,001,820 bp (GRCm38)
  • T to C, chromosome 4 at 98,737,695 bp (GRCm38)
  • T to C, chromosome 4 at 112,625,842 bp (GRCm38)
  • T to C, chromosome 4 at 128,723,269 bp (GRCm38)
  • T to C, chromosome 4 at 132,370,563 bp (GRCm38)
  • T to C, chromosome 4 at 132,579,302 bp (GRCm38)
  • T to C, chromosome 5 at 34,217,444 bp (GRCm38)
  • A to T, chromosome 5 at 114,634,561 bp (GRCm38)
  • C to T, chromosome 5 at 136,057,222 bp (GRCm38)
  • A to T, chromosome 5 at 137,354,562 bp (GRCm38)
  • T to A, chromosome 6 at 23,688,928 bp (GRCm38)
  • C to T, chromosome 6 at 53,974,794 bp (GRCm38)
  • T to C, chromosome 6 at 71,954,771 bp (GRCm38)
  • A to G, chromosome 6 at 119,271,515 bp (GRCm38)
  • C to T, chromosome 7 at 43,938,366 bp (GRCm38)
  • G to A, chromosome 7 at 45,710,913 bp (GRCm38)
  • A to G, chromosome 7 at 55,900,010 bp (GRCm38)
  • A to G, chromosome 7 at 80,343,580 bp (GRCm38)
  • A to G, chromosome 7 at 85,415,489 bp (GRCm38)
  • A to C, chromosome 7 at 128,009,143 bp (GRCm38)
  • A to G, chromosome 8 at 13,225,279 bp (GRCm38)
  • A to T, chromosome 8 at 28,675,420 bp (GRCm38)
  • A to T, chromosome 8 at 61,720,537 bp (GRCm38)
  • G to A, chromosome 8 at 88,318,047 bp (GRCm38)
  • A to G, chromosome 8 at 123,080,669 bp (GRCm38)
  • T to A, chromosome 9 at 14,593,024 bp (GRCm38)
  • G to A, chromosome 9 at 21,593,360 bp (GRCm38)
  • T to A, chromosome 9 at 51,057,537 bp (GRCm38)
  • T to A, chromosome 9 at 75,651,673 bp (GRCm38)
  • A to T, chromosome 9 at 76,084,450 bp (GRCm38)
  • A to T, chromosome 9 at 78,669,760 bp (GRCm38)
  • A to T, chromosome 9 at 106,225,553 bp (GRCm38)
  • C to T, chromosome 10 at 21,154,713 bp (GRCm38)
  • C to T, chromosome 10 at 26,336,257 bp (GRCm38)
  • T to C, chromosome 10 at 63,337,078 bp (GRCm38)
  • T to A, chromosome 10 at 80,154,441 bp (GRCm38)
  • T to A, chromosome 10 at 127,546,938 bp (GRCm38)
  • T to G, chromosome 10 at 127,689,358 bp (GRCm38)
  • A to G, chromosome 11 at 4,039,977 bp (GRCm38)
  • T to C, chromosome 11 at 8,450,094 bp (GRCm38)
  • A to T, chromosome 11 at 58,287,470 bp (GRCm38)
  • T to A, chromosome 11 at 62,951,298 bp (GRCm38)
  • T to C, chromosome 11 at 67,186,696 bp (GRCm38)
  • G to T, chromosome 11 at 69,678,259 bp (GRCm38)
  • A to C, chromosome 11 at 78,225,786 bp (GRCm38)
  • A to G, chromosome 11 at 106,286,466 bp (GRCm38)
  • T to C, chromosome 12 at 49,384,537 bp (GRCm38)
  • T to C, chromosome 12 at 69,341,319 bp (GRCm38)
  • T to A, chromosome 12 at 75,593,289 bp (GRCm38)
  • A to G, chromosome 13 at 22,475,500 bp (GRCm38)
  • A to T, chromosome 13 at 24,952,917 bp (GRCm38)
  • G to A, chromosome 13 at 55,075,839 bp (GRCm38)
  • A to T, chromosome 13 at 112,914,354 bp (GRCm38)
  • C to T, chromosome 14 at 69,190,778 bp (GRCm38)
  • A to G, chromosome 14 at 70,519,898 bp (GRCm38)
  • T to A, chromosome 15 at 98,897,876 bp (GRCm38)
  • A to C, chromosome 16 at 4,713,767 bp (GRCm38)
  • T to A, chromosome 16 at 14,706,928 bp (GRCm38)
  • A to G, chromosome 17 at 84,649,709 bp (GRCm38)
  • A to C, chromosome 17 at 86,826,562 bp (GRCm38)
  • A to G, chromosome 18 at 37,011,459 bp (GRCm38)
  • C to T, chromosome 19 at 40,608,248 bp (GRCm38)
  • A to T, chromosome 19 at 44,107,350 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9228 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069060-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.