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Strain Name:
C57BL/6J-MtgxR9448Btlr/Mmmh
Stock Number:
069251-MU
Citation ID:
RRID:MMRRC_069251-MU
Other Names:
R9448 (G1)
Major Collection:

Strain Information

Slc12a6
Name: solute carrier family 12, member 6
Synonyms: KCC3, gaxp
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107723
Homologene: 21069
Ptk2
Name: PTK2 protein tyrosine kinase 2
Synonyms: Fadk, FAK, FRNK
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14083
VEGA: 15
HGNC: HGNC:9611
Homologene: 7314
Plcb4
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Znfx1
Name: zinc finger, NFX1-type containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98999
Homologene: 10877
Baz1b
Name: bromodomain adjacent to zinc finger domain, 1B
Synonyms: Wbscr9, WSTF
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22385
HGNC: HGNC:961
Homologene: 22651
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, b2b1454Clo, b2b1752Clo, Kiaa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Pcnt
Name: pericentrin (kendrin)
Synonyms: Pcnt2, m275Asp, m239Asp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18541
VEGA: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 1 at 40,327,444 bp (GRCm38)
  • G to T, chromosome 1 at 75,186,205 bp (GRCm38)
  • T to C, chromosome 1 at 165,153,521 bp (GRCm38)
  • T to C, chromosome 1 at 191,012,209 bp (GRCm38)
  • A to T, chromosome 2 at 24,971,940 bp (GRCm38)
  • A to G, chromosome 2 at 37,201,209 bp (GRCm38)
  • A to G, chromosome 2 at 37,787,761 bp (GRCm38)
  • A to T, chromosome 2 at 87,693,480 bp (GRCm38)
  • T to C, chromosome 2 at 112,349,359 bp (GRCm38)
  • T to C, chromosome 2 at 118,814,494 bp (GRCm38)
  • A to C, chromosome 2 at 120,977,730 bp (GRCm38)
  • G to A, chromosome 2 at 135,910,125 bp (GRCm38)
  • G to C, chromosome 2 at 153,804,941 bp (GRCm38)
  • T to G, chromosome 2 at 167,046,924 bp (GRCm38)
  • A to T, chromosome 3 at 96,854,606 bp (GRCm38)
  • T to C, chromosome 4 at 42,793,440 bp (GRCm38)
  • A to T, chromosome 4 at 42,850,250 bp (GRCm38)
  • G to T, chromosome 4 at 53,694,826 bp (GRCm38)
  • A to T, chromosome 4 at 63,745,068 bp (GRCm38)
  • A to G, chromosome 4 at 120,921,691 bp (GRCm38)
  • C to A, chromosome 4 at 156,236,324 bp (GRCm38)
  • T to C, chromosome 5 at 75,941,909 bp (GRCm38)
  • T to C, chromosome 5 at 135,210,802 bp (GRCm38)
  • T to C, chromosome 6 at 40,887,226 bp (GRCm38)
  • T to C, chromosome 6 at 68,571,172 bp (GRCm38)
  • T to C, chromosome 6 at 83,032,991 bp (GRCm38)
  • A to T, chromosome 6 at 111,358,232 bp (GRCm38)
  • G to A, chromosome 6 at 124,732,808 bp (GRCm38)
  • A to T, chromosome 7 at 23,301,531 bp (GRCm38)
  • CCTTCTCCTTCTTCTCCTTCTTCTCCTTCTTCTCCATCTTCTCCTTCTTC to CCTTCTCCTTCTTCTCCTTCTTCTCCATCTTCTCCTTCTTC, chromosome 7 at 29,247,623 bp (GRCm38)
  • A to T, chromosome 7 at 85,872,819 bp (GRCm38)
  • A to T, chromosome 7 at 87,395,793 bp (GRCm38)
  • G to GCCGGCGGCT, chromosome 7 at 97,579,909 bp (GRCm38)
  • A to T, chromosome 7 at 111,554,442 bp (GRCm38)
  • T to A, chromosome 8 at 45,339,374 bp (GRCm38)
  • T to C, chromosome 8 at 121,128,869 bp (GRCm38)
  • G to T, chromosome 9 at 44,711,249 bp (GRCm38)
  • G to T, chromosome 9 at 103,272,582 bp (GRCm38)
  • A to G, chromosome 9 at 119,552,061 bp (GRCm38)
  • T to C, chromosome 10 at 76,420,526 bp (GRCm38)
  • A to G, chromosome 10 at 81,305,811 bp (GRCm38)
  • T to G, chromosome 10 at 100,559,684 bp (GRCm38)
  • T to A, chromosome 10 at 116,313,914 bp (GRCm38)
  • T to C, chromosome 11 at 7,149,575 bp (GRCm38)
  • T to C, chromosome 11 at 22,137,881 bp (GRCm38)
  • T to C, chromosome 11 at 60,715,567 bp (GRCm38)
  • TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC to TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC, chromosome 11 at 100,189,077 bp (GRCm38)
  • C to T, chromosome 11 at 103,208,822 bp (GRCm38)
  • T to C, chromosome 11 at 116,447,265 bp (GRCm38)
  • T to A, chromosome 12 at 65,061,260 bp (GRCm38)
  • A to G, chromosome 12 at 83,842,216 bp (GRCm38)
  • A to G, chromosome 13 at 21,448,261 bp (GRCm38)
  • T to A, chromosome 13 at 52,508,086 bp (GRCm38)
  • T to A, chromosome 13 at 116,902,824 bp (GRCm38)
  • T to C, chromosome 14 at 45,331,026 bp (GRCm38)
  • T to C, chromosome 15 at 47,596,919 bp (GRCm38)
  • T to A, chromosome 15 at 73,343,192 bp (GRCm38)
  • T to C, chromosome 15 at 101,645,227 bp (GRCm38)
  • C to A, chromosome 16 at 18,221,694 bp (GRCm38)
  • T to A, chromosome 16 at 43,938,975 bp (GRCm38)
  • T to A, chromosome 16 at 44,111,509 bp (GRCm38)
  • T to C, chromosome 17 at 6,198,673 bp (GRCm38)
  • T to C, chromosome 17 at 43,494,978 bp (GRCm38)
  • T to C, chromosome 17 at 43,625,676 bp (GRCm38)
  • C to T, chromosome 17 at 75,359,460 bp (GRCm38)
  • T to C, chromosome 17 at 78,760,586 bp (GRCm38)
  • A to G, chromosome 18 at 21,084,142 bp (GRCm38)
  • G to A, chromosome 18 at 38,197,439 bp (GRCm38)
  • T to A, chromosome 19 at 11,414,953 bp (GRCm38)
  • T to C, chromosome 19 at 25,545,891 bp (GRCm38)
  • A to G, chromosome Y at 2,109,904 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9448 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069251-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.