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Strain Name:
C57BL/6J-MtgxR9483Btlr/Mmmh
Stock Number:
069286-MU
Citation ID:
RRID:MMRRC_069286-MU
Other Names:
R9483 (G1)
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22359
Homologene: 443
Zfp423
Name: zinc finger protein 423
Synonyms: Roaz, ataxia1, Zfp104, Ebfaz
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94187
Homologene: 9010
Tmed10
Name: transmembrane p24 trafficking protein 10
Synonyms: 1110014C03Rik, p24delta1, p23
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68581
VEGA: 12
Homologene: 4972
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to G, chromosome 1 at 37,631,415 bp (GRCm38)
  • A to G, chromosome 1 at 131,270,982 bp (GRCm38)
  • T to C, chromosome 1 at 131,867,770 bp (GRCm38)
  • A to G, chromosome 1 at 170,207,344 bp (GRCm38)
  • A to G, chromosome 1 at 180,745,156 bp (GRCm38)
  • T to C, chromosome 1 at 188,198,651 bp (GRCm38)
  • T to C, chromosome 2 at 29,079,480 bp (GRCm38)
  • T to G, chromosome 2 at 31,430,363 bp (GRCm38)
  • T to C, chromosome 2 at 71,849,490 bp (GRCm38)
  • A to T, chromosome 2 at 87,093,046 bp (GRCm38)
  • T to A, chromosome 2 at 104,257,218 bp (GRCm38)
  • T to C, chromosome 2 at 109,289,687 bp (GRCm38)
  • T to A, chromosome 2 at 162,948,814 bp (GRCm38)
  • T to C, chromosome 2 at 173,008,148 bp (GRCm38)
  • A to T, chromosome 3 at 89,086,806 bp (GRCm38)
  • T to C, chromosome 3 at 101,439,501 bp (GRCm38)
  • T to A, chromosome 3 at 101,439,588 bp (GRCm38)
  • T to A, chromosome 3 at 107,471,456 bp (GRCm38)
  • A to T, chromosome 3 at 122,085,626 bp (GRCm38)
  • C to T, chromosome 3 at 159,622,681 bp (GRCm38)
  • A to G, chromosome 4 at 43,415,897 bp (GRCm38)
  • A to T, chromosome 4 at 53,060,351 bp (GRCm38)
  • C to A, chromosome 4 at 62,657,117 bp (GRCm38)
  • T to A, chromosome 4 at 106,362,182 bp (GRCm38)
  • A to G, chromosome 4 at 111,890,221 bp (GRCm38)
  • G to T, chromosome 4 at 116,596,947 bp (GRCm38)
  • A to T, chromosome 4 at 122,857,574 bp (GRCm38)
  • C to A, chromosome 4 at 127,233,872 bp (GRCm38)
  • T to A, chromosome 4 at 132,335,497 bp (GRCm38)
  • A to T, chromosome 4 at 154,017,463 bp (GRCm38)
  • C to A, chromosome 5 at 70,842,215 bp (GRCm38)
  • A to T, chromosome 5 at 113,191,144 bp (GRCm38)
  • T to C, chromosome 5 at 117,989,207 bp (GRCm38)
  • A to G, chromosome 5 at 123,174,472 bp (GRCm38)
  • A to T, chromosome 5 at 138,230,329 bp (GRCm38)
  • A to G, chromosome 5 at 148,295,490 bp (GRCm38)
  • T to A, chromosome 6 at 5,486,716 bp (GRCm38)
  • G to T, chromosome 6 at 28,418,090 bp (GRCm38)
  • A to G, chromosome 7 at 16,152,855 bp (GRCm38)
  • G to A, chromosome 7 at 28,458,758 bp (GRCm38)
  • GGCGGC to GGCGGCGGCAGCGGC, chromosome 7 at 97,579,930 bp (GRCm38)
  • T to C, chromosome 7 at 103,629,724 bp (GRCm38)
  • A to G, chromosome 7 at 105,743,855 bp (GRCm38)
  • A to G, chromosome 7 at 119,783,943 bp (GRCm38)
  • A to G, chromosome 7 at 122,582,440 bp (GRCm38)
  • G to T, chromosome 7 at 141,811,728 bp (GRCm38)
  • C to T, chromosome 8 at 12,851,087 bp (GRCm38)
  • C to T, chromosome 8 at 13,580,033 bp (GRCm38)
  • C to T, chromosome 8 at 71,650,577 bp (GRCm38)
  • T to C, chromosome 8 at 83,722,287 bp (GRCm38)
  • T to A, chromosome 8 at 87,781,097 bp (GRCm38)
  • C to T, chromosome 8 at 105,202,491 bp (GRCm38)
  • C to A, chromosome 9 at 49,035,737 bp (GRCm38)
  • T to C, chromosome 9 at 53,621,174 bp (GRCm38)
  • T to C, chromosome 9 at 67,392,487 bp (GRCm38)
  • T to C, chromosome 9 at 108,305,743 bp (GRCm38)
  • A to T, chromosome 10 at 19,632,794 bp (GRCm38)
  • A to G, chromosome 10 at 33,914,422 bp (GRCm38)
  • A to T, chromosome 11 at 46,687,062 bp (GRCm38)
  • G to T, chromosome 11 at 77,393,150 bp (GRCm38)
  • A to G, chromosome 11 at 101,126,060 bp (GRCm38)
  • G to T, chromosome 12 at 80,956,090 bp (GRCm38)
  • T to C, chromosome 12 at 85,350,847 bp (GRCm38)
  • T to A, chromosome 12 at 111,833,776 bp (GRCm38)
  • T to C, chromosome 14 at 51,411,184 bp (GRCm38)
  • T to C, chromosome 14 at 57,124,054 bp (GRCm38)
  • T to C, chromosome 14 at 62,606,651 bp (GRCm38)
  • T to A, chromosome 15 at 80,211,440 bp (GRCm38)
  • G to T, chromosome 15 at 81,366,386 bp (GRCm38)
  • T to C, chromosome 15 at 101,573,803 bp (GRCm38)
  • A to G, chromosome 17 at 23,996,747 bp (GRCm38)
  • A to T, chromosome 17 at 40,578,981 bp (GRCm38)
  • A to G, chromosome 17 at 75,500,722 bp (GRCm38)
  • A to G, chromosome 18 at 36,753,582 bp (GRCm38)
  • T to A, chromosome 18 at 37,686,693 bp (GRCm38)
  • G to T, chromosome 19 at 4,739,946 bp (GRCm38)
  • A to T, chromosome 19 at 27,246,631 bp (GRCm38)
  • G to T, chromosome 19 at 46,330,960 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9483 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069286-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.