Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR9517Btlr/Mmmh
Stock Number:
069319-MU
Citation ID:
RRID:MMRRC_069319-MU
Other Names:
R9517 (G1)
Major Collection:

Strain Information

Mtrr
Name: 5-methyltetrahydrofolate-homocysteine methyltransferase reductase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 210009
VEGA: 13
HGNC: HGNC:7473
Homologene: 11419
Icos
Name: inducible T cell co-stimulator
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54167
HGNC: HGNC:5351
Homologene: 8097
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Chst5
Name: carbohydrate sulfotransferase 5
Synonyms: I-GlcNAc6ST, GST-4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56773
Homologene: 56927
Hap1
Name: huntingtin-associated protein 1
Synonyms: HAP-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15114
HGNC: HGNC:4812
Homologene: 2935
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 60,993,735 bp (GRCm38)
  • T to A, chromosome 1 at 87,711,131 bp (GRCm38)
  • T to A, chromosome 1 at 139,479,429 bp (GRCm38)
  • C to A, chromosome 1 at 173,203,779 bp (GRCm38)
  • G to A, chromosome 2 at 88,852,603 bp (GRCm38)
  • T to G, chromosome 2 at 111,292,688 bp (GRCm38)
  • A to T, chromosome 2 at 168,182,946 bp (GRCm38)
  • T to C, chromosome 4 at 82,983,477 bp (GRCm38)
  • T to C, chromosome 4 at 134,529,204 bp (GRCm38)
  • T to A, chromosome 4 at 137,404,514 bp (GRCm38)
  • T to A, chromosome 4 at 143,698,360 bp (GRCm38)
  • T to C, chromosome 4 at 149,165,211 bp (GRCm38)
  • A to G, chromosome 5 at 124,469,725 bp (GRCm38)
  • C to T, chromosome 5 at 146,556,596 bp (GRCm38)
  • A to T, chromosome 6 at 4,694,153 bp (GRCm38)
  • A to T, chromosome 6 at 23,113,529 bp (GRCm38)
  • A to T, chromosome 6 at 33,049,382 bp (GRCm38)
  • A to G, chromosome 6 at 57,137,570 bp (GRCm38)
  • A to C, chromosome 6 at 66,805,274 bp (GRCm38)
  • A to G, chromosome 6 at 128,512,154 bp (GRCm38)
  • T to A, chromosome 7 at 4,477,637 bp (GRCm38)
  • A to T, chromosome 7 at 98,071,959 bp (GRCm38)
  • A to G, chromosome 7 at 115,512,735 bp (GRCm38)
  • A to G, chromosome 7 at 120,388,201 bp (GRCm38)
  • C to A, chromosome 8 at 3,717,452 bp (GRCm38)
  • A to G, chromosome 8 at 106,227,298 bp (GRCm38)
  • A to G, chromosome 8 at 111,890,020 bp (GRCm38)
  • A to G, chromosome 8 at 119,626,331 bp (GRCm38)
  • T to C, chromosome 9 at 21,743,944 bp (GRCm38)
  • T to A, chromosome 9 at 38,143,327 bp (GRCm38)
  • T to C, chromosome 9 at 70,802,278 bp (GRCm38)
  • A to G, chromosome 9 at 109,651,824 bp (GRCm38)
  • G to A, chromosome 9 at 121,642,927 bp (GRCm38)
  • C to T, chromosome 10 at 21,154,713 bp (GRCm38)
  • C to T, chromosome 10 at 79,377,807 bp (GRCm38)
  • A to T, chromosome 11 at 98,219,084 bp (GRCm38)
  • A to T, chromosome 11 at 100,349,362 bp (GRCm38)
  • G to A, chromosome 11 at 103,542,590 bp (GRCm38)
  • A to G, chromosome 11 at 118,024,614 bp (GRCm38)
  • T to C, chromosome 12 at 102,368,636 bp (GRCm38)
  • G to T, chromosome 13 at 12,280,431 bp (GRCm38)
  • G to A, chromosome 13 at 49,551,799 bp (GRCm38)
  • A to T, chromosome 13 at 68,580,611 bp (GRCm38)
  • A to G, chromosome 13 at 69,506,940 bp (GRCm38)
  • A to G, chromosome 13 at 75,763,987 bp (GRCm38)
  • A to G, chromosome 13 at 76,062,519 bp (GRCm38)
  • A to G, chromosome 14 at 33,932,565 bp (GRCm38)
  • T to A, chromosome 14 at 37,072,315 bp (GRCm38)
  • C to A, chromosome 14 at 50,515,313 bp (GRCm38)
  • T to C, chromosome 14 at 121,591,824 bp (GRCm38)
  • G to T, chromosome 15 at 4,375,678 bp (GRCm38)
  • G to T, chromosome 15 at 4,798,432 bp (GRCm38)
  • A to T, chromosome 15 at 58,052,416 bp (GRCm38)
  • G to A, chromosome 15 at 99,279,735 bp (GRCm38)
  • T to G, chromosome 16 at 58,433,456 bp (GRCm38)
  • G to A, chromosome 17 at 24,895,059 bp (GRCm38)
  • A to G, chromosome 18 at 12,909,908 bp (GRCm38)
  • C to A, chromosome 18 at 44,661,727 bp (GRCm38)
  • T to C, chromosome 18 at 78,858,107 bp (GRCm38)
  • T to C, chromosome 18 at 80,682,191 bp (GRCm38)
  • A to T, chromosome 19 at 4,118,217 bp (GRCm38)
  • A to T, chromosome 19 at 56,371,845 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9517 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069319-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.