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Strain Name:
C57BL/6J-MtgxR9557Btlr/Mmmh
Stock Number:
069352-MU
Citation ID:
RRID:MMRRC_069352-MU
Other Names:
R9557 (G1)
Major Collection:

Strain Information

Ing4
Name: inhibitor of growth family, member 4
Synonyms: D6Xrf92, p29ING4, D6Wsu147e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28019
Homologene: 22952
Slc6a5
Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms: Glyt2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 104245
Homologene: 37901
Fam110b
Name: family with sequence similarity 110, member B
Synonyms: 1700012H17Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242297
Homologene: 17518
Comp
Name: cartilage oligomeric matrix protein
Synonyms: thrombospondin-5, TSP5
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12845
HGNC: HGNC:2227
Homologene: 74
Ppp4r1
Name: protein phosphatase 4, regulatory subunit 1
Synonyms: Pp4r1, 3110001J10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70351
HGNC: HGNC:9320
Homologene: 81737
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Mllt6
Name: myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6
Synonyms: Af17
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 246198
HGNC: HGNC:7138
Homologene: 31347
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • G to T, chromosome 1 at 58,245,936 bp (GRCm38)
  • A to G, chromosome 1 at 60,235,350 bp (GRCm38)
  • G to T, chromosome 1 at 88,107,098 bp (GRCm38)
  • A to C, chromosome 1 at 153,457,546 bp (GRCm38)
  • A to T, chromosome 2 at 3,474,414 bp (GRCm38)
  • G to A, chromosome 2 at 30,032,522 bp (GRCm38)
  • C to T, chromosome 2 at 76,885,013 bp (GRCm38)
  • C to T, chromosome 2 at 109,996,739 bp (GRCm38)
  • T to C, chromosome 2 at 125,338,538 bp (GRCm38)
  • T to A, chromosome 2 at 164,583,838 bp (GRCm38)
  • G to T, chromosome 3 at 14,588,251 bp (GRCm38)
  • A to T, chromosome 3 at 35,995,827 bp (GRCm38)
  • T to A, chromosome 3 at 59,751,739 bp (GRCm38)
  • T to G, chromosome 3 at 59,877,233 bp (GRCm38)
  • C to T, chromosome 3 at 104,015,157 bp (GRCm38)
  • A to C, chromosome 3 at 104,017,617 bp (GRCm38)
  • A to G, chromosome 3 at 133,485,805 bp (GRCm38)
  • T to C, chromosome 4 at 5,799,064 bp (GRCm38)
  • A to T, chromosome 5 at 3,851,432 bp (GRCm38)
  • A to G, chromosome 5 at 121,321,554 bp (GRCm38)
  • C to A, chromosome 5 at 150,466,316 bp (GRCm38)
  • A to G, chromosome 6 at 125,048,391 bp (GRCm38)
  • T to C, chromosome 7 at 23,702,784 bp (GRCm38)
  • A to T, chromosome 7 at 25,359,086 bp (GRCm38)
  • A to C, chromosome 7 at 26,080,698 bp (GRCm38)
  • T to C, chromosome 7 at 29,204,798 bp (GRCm38)
  • A to G, chromosome 7 at 49,911,726 bp (GRCm38)
  • T to A, chromosome 7 at 101,913,122 bp (GRCm38)
  • T to C, chromosome 7 at 104,580,561 bp (GRCm38)
  • T to C, chromosome 7 at 144,410,110 bp (GRCm38)
  • T to C, chromosome 8 at 22,772,653 bp (GRCm38)
  • T to A, chromosome 8 at 70,377,204 bp (GRCm38)
  • C to T, chromosome 8 at 75,117,540 bp (GRCm38)
  • G to A, chromosome 8 at 84,930,845 bp (GRCm38)
  • A to G, chromosome 8 at 105,142,895 bp (GRCm38)
  • G to A, chromosome 9 at 44,319,830 bp (GRCm38)
  • A to G, chromosome 9 at 122,749,465 bp (GRCm38)
  • G to A, chromosome 10 at 23,900,825 bp (GRCm38)
  • T to A, chromosome 10 at 38,820,874 bp (GRCm38)
  • T to C, chromosome 10 at 49,528,009 bp (GRCm38)
  • A to T, chromosome 11 at 80,353,451 bp (GRCm38)
  • A to G, chromosome 11 at 97,344,203 bp (GRCm38)
  • A to G, chromosome 11 at 97,673,484 bp (GRCm38)
  • A to G, chromosome 11 at 110,306,283 bp (GRCm38)
  • T to A, chromosome 12 at 26,445,522 bp (GRCm38)
  • T to A, chromosome 12 at 72,486,207 bp (GRCm38)
  • A to G, chromosome 12 at 75,632,892 bp (GRCm38)
  • G to A, chromosome 12 at 80,642,797 bp (GRCm38)
  • A to T, chromosome 12 at 81,379,300 bp (GRCm38)
  • G to T, chromosome 13 at 28,952,930 bp (GRCm38)
  • C to T, chromosome 13 at 63,842,122 bp (GRCm38)
  • T to C, chromosome 13 at 64,152,772 bp (GRCm38)
  • C to A, chromosome 13 at 112,348,813 bp (GRCm38)
  • A to G, chromosome 14 at 50,534,095 bp (GRCm38)
  • T to A, chromosome 14 at 103,135,261 bp (GRCm38)
  • G to T, chromosome 15 at 7,212,175 bp (GRCm38)
  • T to C, chromosome 15 at 75,839,296 bp (GRCm38)
  • C to T, chromosome 15 at 79,028,747 bp (GRCm38)
  • A to T, chromosome 16 at 5,239,956 bp (GRCm38)
  • T to C, chromosome 16 at 35,270,957 bp (GRCm38)
  • C to G, chromosome 16 at 87,719,715 bp (GRCm38)
  • G to A, chromosome 17 at 33,242,133 bp (GRCm38)
  • C to A, chromosome 17 at 65,803,263 bp (GRCm38)
  • T to G, chromosome 17 at 75,500,144 bp (GRCm38)
  • T to A, chromosome 17 at 84,994,073 bp (GRCm38)
  • C to A, chromosome 18 at 34,318,359 bp (GRCm38)
  • TAAAATGTTCCAAA to TAAA, chromosome 18 at 68,275,777 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9557 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069352-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.