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Strain Name:
C57BL/6J-MtgxR9598Btlr/Mmmh
Stock Number:
069391-MU
Citation ID:
RRID:MMRRC_069391-MU
Other Names:
R9598 (G1)
Major Collection:

Strain Information

Dnmt3a
Name: DNA methyltransferase 3A
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13435
HGNC: HGNC:2978
Homologene: 7294
Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: alpha4 nAChR, Acra-4, Acra4, a4 nicotinic receptor, alpha4-nAChR
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Dusp16
Name: dual specificity phosphatase 16
Synonyms: MKP-7, MKP7, 3830417M17Rik, D6Ertd213e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70686
Homologene: 15604
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • G to A, chromosome 1 at 6,239,965 bp (GRCm38)
  • A to G, chromosome 1 at 9,745,794 bp (GRCm38)
  • G to A, chromosome 1 at 34,113,933 bp (GRCm38)
  • T to C, chromosome 1 at 46,253,461 bp (GRCm38)
  • A to T, chromosome 1 at 80,648,222 bp (GRCm38)
  • T to C, chromosome 1 at 132,229,295 bp (GRCm38)
  • T to C, chromosome 1 at 133,084,987 bp (GRCm38)
  • T to C, chromosome 1 at 173,923,956 bp (GRCm38)
  • T to C, chromosome 1 at 174,608,742 bp (GRCm38)
  • T to A, chromosome 1 at 180,999,816 bp (GRCm38)
  • A to C, chromosome 2 at 88,149,591 bp (GRCm38)
  • A to T, chromosome 2 at 88,340,477 bp (GRCm38)
  • A to T, chromosome 2 at 111,329,288 bp (GRCm38)
  • T to C, chromosome 2 at 139,977,647 bp (GRCm38)
  • G to A, chromosome 2 at 181,037,471 bp (GRCm38)
  • G to T, chromosome 3 at 32,741,732 bp (GRCm38)
  • A to C, chromosome 3 at 107,219,625 bp (GRCm38)
  • T to C, chromosome 3 at 108,415,262 bp (GRCm38)
  • A to T, chromosome 3 at 153,901,511 bp (GRCm38)
  • C to A, chromosome 4 at 45,815,472 bp (GRCm38)
  • T to C, chromosome 4 at 103,231,407 bp (GRCm38)
  • A to G, chromosome 4 at 118,409,161 bp (GRCm38)
  • T to A, chromosome 4 at 123,116,620 bp (GRCm38)
  • A to G, chromosome 5 at 105,136,874 bp (GRCm38)
  • A to T, chromosome 6 at 85,867,866 bp (GRCm38)
  • T to A, chromosome 6 at 134,718,222 bp (GRCm38)
  • T to A, chromosome 6 at 140,039,758 bp (GRCm38)
  • T to G, chromosome 7 at 20,019,377 bp (GRCm38)
  • G to A, chromosome 7 at 44,312,918 bp (GRCm38)
  • T to C, chromosome 7 at 104,840,511 bp (GRCm38)
  • T to A, chromosome 7 at 112,588,546 bp (GRCm38)
  • A to C, chromosome 7 at 128,676,791 bp (GRCm38)
  • A to G, chromosome 8 at 36,103,915 bp (GRCm38)
  • G to A, chromosome 8 at 47,875,325 bp (GRCm38)
  • G to T, chromosome 8 at 55,940,683 bp (GRCm38)
  • G to T, chromosome 8 at 60,913,027 bp (GRCm38)
  • A to T, chromosome 8 at 93,256,866 bp (GRCm38)
  • T to C, chromosome 8 at 111,021,041 bp (GRCm38)
  • T to C, chromosome 9 at 53,520,081 bp (GRCm38)
  • A to C, chromosome 9 at 121,908,591 bp (GRCm38)
  • T to C, chromosome 10 at 32,402,295 bp (GRCm38)
  • A to T, chromosome 10 at 60,378,795 bp (GRCm38)
  • A to T, chromosome 10 at 88,412,014 bp (GRCm38)
  • A to G, chromosome 10 at 91,158,746 bp (GRCm38)
  • A to G, chromosome 11 at 60,727,165 bp (GRCm38)
  • T to A, chromosome 11 at 115,348,912 bp (GRCm38)
  • G to A, chromosome 11 at 117,153,276 bp (GRCm38)
  • G to A, chromosome 12 at 3,896,997 bp (GRCm38)
  • T to C, chromosome 12 at 84,417,496 bp (GRCm38)
  • C to A, chromosome 12 at 108,898,687 bp (GRCm38)
  • A to G, chromosome 12 at 114,616,137 bp (GRCm38)
  • G to A, chromosome 13 at 65,066,708 bp (GRCm38)
  • C to A, chromosome 13 at 65,073,600 bp (GRCm38)
  • C to A, chromosome 14 at 31,610,240 bp (GRCm38)
  • A to G, chromosome 15 at 12,162,206 bp (GRCm38)
  • T to A, chromosome 15 at 66,912,915 bp (GRCm38)
  • A to T, chromosome 15 at 99,325,329 bp (GRCm38)
  • T to A, chromosome 17 at 68,559,772 bp (GRCm38)
  • A to G, chromosome 18 at 20,539,732 bp (GRCm38)
  • A to G, chromosome 18 at 23,883,650 bp (GRCm38)
  • A to G, chromosome 18 at 37,342,381 bp (GRCm38)
  • A to G, chromosome 19 at 9,003,785 bp (GRCm38)
  • T to A, chromosome 19 at 59,038,303 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9598 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069391-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.