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Strain Name:
C57BL/6J-MtgxR9664Btlr/Mmmh
Stock Number:
069457-MU
Citation ID:
RRID:MMRRC_069457-MU
Other Names:
R9664 (G1)
Major Collection:

Strain Information

Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269473
Homologene: 8882
Ppm1a
Name: protein phosphatase 1A, magnesium dependent, alpha isoform
Synonyms: Mpp alpha, MMPa-2, MPPa-1, 2900017D14Rik, 2310003C21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19042
VEGA: 12
HGNC: HGNC:9275
Homologene: 56428
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Zfp113
Name: zinc finger protein 113
Synonyms: 4732456B05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56314
Homologene: 50027
Usp38
Name: ubiquitin specific peptidase 38
Synonyms: 4631402N15Rik, 4833420O05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74841
Homologene: 12367
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 1 at 12,820,802 bp (GRCm38)
  • T to C, chromosome 1 at 34,181,655 bp (GRCm38)
  • A to G, chromosome 1 at 74,254,875 bp (GRCm38)
  • A to T, chromosome 1 at 75,482,628 bp (GRCm38)
  • A to G, chromosome 1 at 104,934,340 bp (GRCm38)
  • C to T, chromosome 2 at 40,874,992 bp (GRCm38)
  • C to T, chromosome 2 at 76,739,501 bp (GRCm38)
  • T to C, chromosome 2 at 76,951,480 bp (GRCm38)
  • C to A, chromosome 2 at 114,140,915 bp (GRCm38)
  • T to C, chromosome 3 at 87,535,865 bp (GRCm38)
  • A to G, chromosome 3 at 104,464,240 bp (GRCm38)
  • A to G, chromosome 3 at 142,312,297 bp (GRCm38)
  • A to T, chromosome 3 at 144,797,814 bp (GRCm38)
  • T to C, chromosome 3 at 146,042,839 bp (GRCm38)
  • T to A, chromosome 4 at 3,585,964 bp (GRCm38)
  • A to G, chromosome 4 at 101,618,624 bp (GRCm38)
  • C to T, chromosome 4 at 126,323,651 bp (GRCm38)
  • A to G, chromosome 4 at 137,296,618 bp (GRCm38)
  • C to T, chromosome 4 at 137,539,576 bp (GRCm38)
  • A to T, chromosome 4 at 148,625,294 bp (GRCm38)
  • A to C, chromosome 5 at 67,732,181 bp (GRCm38)
  • T to C, chromosome 5 at 110,340,306 bp (GRCm38)
  • G to T, chromosome 5 at 122,382,243 bp (GRCm38)
  • A to T, chromosome 5 at 123,159,983 bp (GRCm38)
  • A to T, chromosome 5 at 123,180,885 bp (GRCm38)
  • A to T, chromosome 5 at 138,150,626 bp (GRCm38)
  • A to G, chromosome 5 at 145,243,632 bp (GRCm38)
  • A to G, chromosome 5 at 150,359,023 bp (GRCm38)
  • T to C, chromosome 6 at 29,457,215 bp (GRCm38)
  • A to T, chromosome 6 at 34,889,026 bp (GRCm38)
  • A to G, chromosome 6 at 87,111,926 bp (GRCm38)
  • A to G, chromosome 7 at 12,176,655 bp (GRCm38)
  • A to G, chromosome 7 at 23,418,861 bp (GRCm38)
  • A to G, chromosome 7 at 29,059,667 bp (GRCm38)
  • C to T, chromosome 7 at 48,021,525 bp (GRCm38)
  • A to G, chromosome 7 at 56,170,590 bp (GRCm38)
  • T to C, chromosome 7 at 108,868,356 bp (GRCm38)
  • C to A, chromosome 7 at 126,692,526 bp (GRCm38)
  • C to A, chromosome 7 at 141,855,542 bp (GRCm38)
  • A to T, chromosome 8 at 45,241,529 bp (GRCm38)
  • A to T, chromosome 8 at 45,823,751 bp (GRCm38)
  • A to G, chromosome 8 at 71,678,722 bp (GRCm38)
  • A to G, chromosome 8 at 81,014,535 bp (GRCm38)
  • C to A, chromosome 8 at 84,903,432 bp (GRCm38)
  • A to T, chromosome 8 at 110,494,333 bp (GRCm38)
  • T to C, chromosome 9 at 21,305,687 bp (GRCm38)
  • C to T, chromosome 9 at 44,848,805 bp (GRCm38)
  • T to C, chromosome 9 at 58,086,572 bp (GRCm38)
  • T to C, chromosome 9 at 105,781,055 bp (GRCm38)
  • C to T, chromosome 9 at 108,983,581 bp (GRCm38)
  • T to C, chromosome 9 at 110,548,502 bp (GRCm38)
  • T to A, chromosome 10 at 52,120,835 bp (GRCm38)
  • A to T, chromosome 10 at 60,777,543 bp (GRCm38)
  • T to C, chromosome 10 at 80,712,805 bp (GRCm38)
  • G to A, chromosome 10 at 80,788,527 bp (GRCm38)
  • T to C, chromosome 11 at 3,294,562 bp (GRCm38)
  • C to A, chromosome 11 at 29,455,484 bp (GRCm38)
  • A to T, chromosome 11 at 58,220,046 bp (GRCm38)
  • A to T, chromosome 11 at 62,584,559 bp (GRCm38)
  • G to T, chromosome 11 at 79,443,907 bp (GRCm38)
  • G to T, chromosome 11 at 96,100,278 bp (GRCm38)
  • A to C, chromosome 11 at 102,868,596 bp (GRCm38)
  • G to A, chromosome 11 at 119,441,968 bp (GRCm38)
  • T to A, chromosome 12 at 25,056,896 bp (GRCm38)
  • T to A, chromosome 12 at 65,090,984 bp (GRCm38)
  • G to A, chromosome 12 at 72,790,677 bp (GRCm38)
  • T to G, chromosome 12 at 111,979,479 bp (GRCm38)
  • A to G, chromosome 12 at 112,774,929 bp (GRCm38)
  • A to G, chromosome 13 at 21,959,926 bp (GRCm38)
  • T to C, chromosome 13 at 43,425,043 bp (GRCm38)
  • A to T, chromosome 13 at 81,522,169 bp (GRCm38)
  • A to T, chromosome 13 at 81,595,252 bp (GRCm38)
  • T to A, chromosome 13 at 93,720,615 bp (GRCm38)
  • T to A, chromosome 14 at 14,407,963 bp (GRCm38)
  • C to T, chromosome 14 at 55,710,984 bp (GRCm38)
  • G to A, chromosome 14 at 55,718,918 bp (GRCm38)
  • A to G, chromosome 14 at 56,485,179 bp (GRCm38)
  • G to A, chromosome 15 at 76,594,183 bp (GRCm38)
  • G to T, chromosome 15 at 79,485,019 bp (GRCm38)
  • A to T, chromosome 15 at 81,006,734 bp (GRCm38)
  • T to A, chromosome 16 at 20,228,741 bp (GRCm38)
  • A to G, chromosome 16 at 36,408,276 bp (GRCm38)
  • T to C, chromosome 16 at 37,027,814 bp (GRCm38)
  • G to T, chromosome 16 at 59,613,836 bp (GRCm38)
  • A to T, chromosome 16 at 96,316,003 bp (GRCm38)
  • A to G, chromosome 17 at 34,565,627 bp (GRCm38)
  • A to C, chromosome 17 at 71,562,518 bp (GRCm38)
  • A to T, chromosome 17 at 84,712,834 bp (GRCm38)
  • T to C, chromosome 18 at 34,704,538 bp (GRCm38)
  • T to G, chromosome 18 at 36,647,825 bp (GRCm38)
  • A to G, chromosome 18 at 80,142,722 bp (GRCm38)
  • A to G, chromosome 19 at 4,004,390 bp (GRCm38)
  • A to T, chromosome 19 at 6,281,232 bp (GRCm38)
  • G to T, chromosome 19 at 6,831,986 bp (GRCm38)
  • G to A, chromosome 19 at 10,638,117 bp (GRCm38)
  • G to A, chromosome 19 at 13,620,001 bp (GRCm38)
  • G to A, chromosome 19 at 34,821,794 bp (GRCm38)
  • A to T, chromosome 19 at 41,308,528 bp (GRCm38)
  • T to C, chromosome 19 at 42,267,023 bp (GRCm38)
  • T to C, chromosome 19 at 42,564,232 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9664 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069457-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.