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Strain Name:
C57BL/6J-MtgxR9716Btlr/Mmmh
Stock Number:
069963-MU
Citation ID:
RRID:MMRRC_069963-MU
Other Names:
R9716 (G1)
Major Collection:

Strain Information

Gulo
Name: gulonolactone (L-) oxidase
Synonyms: L-gulono-gamma-lactone oxidase, sfx
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268756
VEGA: 14
HGNC: HGNC:4695
Homologene: 6566
Itpk1
Name: inositol 1,3,4-triphosphate 5/6 kinase
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217837
HGNC: HGNC:6177
Homologene: 8588
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Prkacb
Name: protein kinase, cAMP dependent, catalytic, beta
Synonyms: cAMP-dependent protein kinase C beta, Pkacb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18749
HGNC: HGNC:9381
Homologene: 121718
Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra-5, Acra5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 1 at 4,142,610 bp (GRCm38)
  • A to G, chromosome 1 at 9,745,723 bp (GRCm38)
  • A to T, chromosome 1 at 87,697,469 bp (GRCm38)
  • T to A, chromosome 1 at 174,431,075 bp (GRCm38)
  • A to T, chromosome 1 at 175,795,092 bp (GRCm38)
  • T to A, chromosome 2 at 32,299,275 bp (GRCm38)
  • C to T, chromosome 2 at 36,997,278 bp (GRCm38)
  • C to T, chromosome 2 at 61,804,733 bp (GRCm38)
  • T to A, chromosome 2 at 87,285,147 bp (GRCm38)
  • T to A, chromosome 2 at 110,771,031 bp (GRCm38)
  • A to G, chromosome 2 at 152,976,216 bp (GRCm38)
  • C to T, chromosome 3 at 19,231,003 bp (GRCm38)
  • T to A, chromosome 3 at 125,438,562 bp (GRCm38)
  • T to C, chromosome 3 at 146,757,720 bp (GRCm38)
  • T to C, chromosome 4 at 19,890,834 bp (GRCm38)
  • G to A, chromosome 5 at 34,854,675 bp (GRCm38)
  • T to C, chromosome 5 at 109,297,358 bp (GRCm38)
  • A to G, chromosome 5 at 114,670,409 bp (GRCm38)
  • A to G, chromosome 5 at 148,076,654 bp (GRCm38)
  • A to T, chromosome 6 at 3,687,468 bp (GRCm38)
  • G to T, chromosome 6 at 22,959,651 bp (GRCm38)
  • G to A, chromosome 6 at 24,604,183 bp (GRCm38)
  • T to C, chromosome 6 at 47,554,207 bp (GRCm38)
  • G to T, chromosome 6 at 85,601,252 bp (GRCm38)
  • T to A, chromosome 6 at 124,832,209 bp (GRCm38)
  • T to C, chromosome 6 at 128,620,276 bp (GRCm38)
  • T to C, chromosome 6 at 130,323,639 bp (GRCm38)
  • T to C, chromosome 7 at 28,386,883 bp (GRCm38)
  • A to T, chromosome 7 at 44,500,981 bp (GRCm38)
  • CGGC to CGGCGGCGGAGGC, chromosome 7 at 97,579,932 bp (GRCm38)
  • T to C, chromosome 7 at 102,075,907 bp (GRCm38)
  • T to A, chromosome 8 at 24,714,258 bp (GRCm38)
  • T to A, chromosome 8 at 41,275,131 bp (GRCm38)
  • C to A, chromosome 8 at 70,259,720 bp (GRCm38)
  • T to G, chromosome 8 at 85,878,169 bp (GRCm38)
  • T to C, chromosome 8 at 95,344,057 bp (GRCm38)
  • A to G, chromosome 9 at 21,831,122 bp (GRCm38)
  • C to T, chromosome 9 at 37,607,625 bp (GRCm38)
  • T to A, chromosome 9 at 45,149,341 bp (GRCm38)
  • A to G, chromosome 9 at 55,004,635 bp (GRCm38)
  • C to T, chromosome 9 at 96,045,579 bp (GRCm38)
  • C to T, chromosome 10 at 68,740,960 bp (GRCm38)
  • C to T, chromosome 10 at 75,986,109 bp (GRCm38)
  • T to A, chromosome 10 at 77,295,705 bp (GRCm38)
  • T to A, chromosome 10 at 77,606,958 bp (GRCm38)
  • T to A, chromosome 10 at 77,853,268 bp (GRCm38)
  • T to C, chromosome 10 at 82,660,810 bp (GRCm38)
  • C to T, chromosome 10 at 88,503,584 bp (GRCm38)
  • T to C, chromosome 10 at 121,611,487 bp (GRCm38)
  • T to A, chromosome 11 at 31,081,460 bp (GRCm38)
  • T to C, chromosome 11 at 98,327,618 bp (GRCm38)
  • T to C, chromosome 12 at 24,710,447 bp (GRCm38)
  • T to A, chromosome 12 at 65,158,563 bp (GRCm38)
  • C to T, chromosome 12 at 102,606,088 bp (GRCm38)
  • T to A, chromosome 12 at 118,060,413 bp (GRCm38)
  • A to T, chromosome 13 at 21,363,768 bp (GRCm38)
  • T to C, chromosome 13 at 55,310,500 bp (GRCm38)
  • T to C, chromosome 13 at 112,945,078 bp (GRCm38)
  • A to G, chromosome 14 at 25,695,497 bp (GRCm38)
  • T to A, chromosome 14 at 43,750,852 bp (GRCm38)
  • T to C, chromosome 14 at 65,996,899 bp (GRCm38)
  • A to T, chromosome 15 at 6,496,949 bp (GRCm38)
  • G to A, chromosome 15 at 76,110,326 bp (GRCm38)
  • T to C, chromosome 15 at 98,843,402 bp (GRCm38)
  • T to C, chromosome 15 at 102,082,008 bp (GRCm38)
  • T to C, chromosome 17 at 34,021,446 bp (GRCm38)
  • G to T, chromosome 17 at 37,211,464 bp (GRCm38)
  • G to A, chromosome 17 at 45,433,574 bp (GRCm38)
  • A to G, chromosome 17 at 88,743,018 bp (GRCm38)
  • C to A, chromosome 18 at 5,062,370 bp (GRCm38)
  • T to C, chromosome 18 at 12,450,403 bp (GRCm38)
  • T to C, chromosome 18 at 34,629,175 bp (GRCm38)
  • T to C, chromosome 18 at 46,524,494 bp (GRCm38)
  • C to T, chromosome 19 at 4,262,409 bp (GRCm38)
  • A to G, chromosome 19 at 6,536,735 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9716 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069963-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.