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Strain Name:
C57BL/6J-MtgxR9720Btlr/Mmmh
Stock Number:
069967-MU
Citation ID:
RRID:MMRRC_069967-MU
Other Names:
R9720 (G1)
Major Collection:

Strain Information

Sall4
Name: spalt like transcription factor 4
Synonyms: 5730441M18Rik, Tex20, C330011P20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99377
Homologene: 10716
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Nphs1
Name: nephrosis 1, nephrin
Synonyms: nephrin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54631
HGNC: HGNC:7908
Homologene: 20974
Ppil2
Name: peptidylprolyl isomerase (cyclophilin)-like 2
Synonyms: C130078A06Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66053
HGNC: HGNC:9261
Homologene: 8643
Ggta1
Name: glycoprotein galactosyltransferase alpha 1, 3
Synonyms: GALT, alpha Gal, Gal, glycoprotein alpha galactosyl transferase 1, alpha3GalT, Ggta-1, Ggta
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14594
HGNC: HGNC:4253
Homologene: 7730
Hap1
Name: huntingtin-associated protein 1
Synonyms: HAP-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15114
HGNC: HGNC:4812
Homologene: 2935
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 66,644,326 bp (GRCm38)
  • A to T, chromosome 1 at 107,155,939 bp (GRCm38)
  • G to T, chromosome 2 at 35,413,406 bp (GRCm38)
  • T to C, chromosome 2 at 69,824,460 bp (GRCm38)
  • A to G, chromosome 2 at 131,058,316 bp (GRCm38)
  • A to T, chromosome 2 at 150,117,277 bp (GRCm38)
  • A to G, chromosome 2 at 155,408,384 bp (GRCm38)
  • G to C, chromosome 2 at 168,750,240 bp (GRCm38)
  • T to C, chromosome 2 at 172,427,648 bp (GRCm38)
  • A to G, chromosome 3 at 29,662,431 bp (GRCm38)
  • A to G, chromosome 3 at 30,659,928 bp (GRCm38)
  • G to A, chromosome 3 at 94,779,311 bp (GRCm38)
  • T to C, chromosome 3 at 97,695,971 bp (GRCm38)
  • C to T, chromosome 3 at 129,826,848 bp (GRCm38)
  • A to C, chromosome 5 at 31,203,570 bp (GRCm38)
  • T to A, chromosome 5 at 108,319,934 bp (GRCm38)
  • C to T, chromosome 5 at 110,337,043 bp (GRCm38)
  • T to C, chromosome 5 at 142,212,041 bp (GRCm38)
  • T to C, chromosome 6 at 116,557,055 bp (GRCm38)
  • G to A, chromosome 6 at 118,521,941 bp (GRCm38)
  • A to G, chromosome 6 at 120,958,277 bp (GRCm38)
  • G to A, chromosome 6 at 125,307,385 bp (GRCm38)
  • G to T, chromosome 6 at 132,711,671 bp (GRCm38)
  • T to C, chromosome 7 at 3,159,432 bp (GRCm38)
  • G to A, chromosome 7 at 19,909,196 bp (GRCm38)
  • T to C, chromosome 7 at 30,466,074 bp (GRCm38)
  • C to A, chromosome 7 at 110,777,849 bp (GRCm38)
  • T to A, chromosome 7 at 119,787,753 bp (GRCm38)
  • C to A, chromosome 7 at 133,723,128 bp (GRCm38)
  • A to T, chromosome 7 at 144,128,400 bp (GRCm38)
  • G to A, chromosome 8 at 47,824,751 bp (GRCm38)
  • T to G, chromosome 8 at 82,331,979 bp (GRCm38)
  • G to A, chromosome 8 at 116,954,914 bp (GRCm38)
  • G to A, chromosome 9 at 27,309,514 bp (GRCm38)
  • A to G, chromosome 9 at 31,854,111 bp (GRCm38)
  • A to G, chromosome 10 at 23,992,835 bp (GRCm38)
  • A to T, chromosome 10 at 69,989,505 bp (GRCm38)
  • T to A, chromosome 10 at 81,333,184 bp (GRCm38)
  • T to A, chromosome 10 at 89,805,357 bp (GRCm38)
  • G to A, chromosome 10 at 102,512,508 bp (GRCm38)
  • T to C, chromosome 10 at 129,776,712 bp (GRCm38)
  • A to G, chromosome 11 at 29,463,813 bp (GRCm38)
  • T to C, chromosome 11 at 41,971,846 bp (GRCm38)
  • T to C, chromosome 11 at 50,776,127 bp (GRCm38)
  • T to G, chromosome 11 at 84,263,357 bp (GRCm38)
  • T to A, chromosome 11 at 94,411,471 bp (GRCm38)
  • T to A, chromosome 11 at 100,355,870 bp (GRCm38)
  • C to T, chromosome 11 at 115,012,292 bp (GRCm38)
  • T to A, chromosome 11 at 118,393,257 bp (GRCm38)
  • A to G, chromosome 12 at 76,438,524 bp (GRCm38)
  • A to C, chromosome 12 at 109,593,448 bp (GRCm38)
  • A to G, chromosome 13 at 81,592,574 bp (GRCm38)
  • T to A, chromosome 14 at 52,795,620 bp (GRCm38)
  • C to A, chromosome 14 at 53,757,829 bp (GRCm38)
  • A to G, chromosome 14 at 68,121,344 bp (GRCm38)
  • A to G, chromosome 15 at 27,847,409 bp (GRCm38)
  • A to G, chromosome 15 at 76,616,730 bp (GRCm38)
  • A to T, chromosome 15 at 85,818,296 bp (GRCm38)
  • T to A, chromosome 15 at 101,679,791 bp (GRCm38)
  • T to C, chromosome 16 at 16,269,720 bp (GRCm38)
  • T to C, chromosome 16 at 17,093,026 bp (GRCm38)
  • C to T, chromosome 17 at 5,990,309 bp (GRCm38)
  • G to T, chromosome 17 at 25,155,497 bp (GRCm38)
  • A to G, chromosome 17 at 28,807,356 bp (GRCm38)
  • A to G, chromosome 18 at 36,787,554 bp (GRCm38)
  • A to G, chromosome 19 at 10,608,360 bp (GRCm38)
  • A to T, chromosome 19 at 38,169,439 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9720 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069967-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.