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Strain Name:
C57BL/6J-MtgxR9762Btlr/Mmmh
Stock Number:
070007-MU
Citation ID:
RRID:MMRRC_070007-MU
Other Names:
R9762 (G1)
Major Collection:

Strain Information

Cdkn2d
Name: cyclin dependent kinase inhibitor 2D
Synonyms: p19, INK4d, p19INK4d, INK4d
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12581
VEGA: 9
HGNC: HGNC:1790
Homologene: 36081
Pdgfc
Name: platelet-derived growth factor, C polypeptide
Synonyms: 1110064L01Rik, PDGF-C
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54635
HGNC: HGNC:8801
Homologene: 9423
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Acap2
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Synonyms: 9530039J15Rik, Centb2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78618
VEGA: 16
Homologene: 8182
Ski
Name: ski sarcoma viral oncogene homolog (avian)
Synonyms: 2610001A11Rik, 2310012I02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20481
Homologene: 31124
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • ATT to ATTT, chromosome 1 at 75,133,281 bp (GRCm38)
  • G to T, chromosome 1 at 158,857,378 bp (GRCm38)
  • A to G, chromosome 1 at 180,334,187 bp (GRCm38)
  • T to C, chromosome 2 at 70,009,153 bp (GRCm38)
  • A to T, chromosome 2 at 104,664,339 bp (GRCm38)
  • G to A, chromosome 2 at 174,298,846 bp (GRCm38)
  • T to C, chromosome 3 at 40,772,625 bp (GRCm38)
  • A to T, chromosome 3 at 59,329,685 bp (GRCm38)
  • A to G, chromosome 3 at 81,037,485 bp (GRCm38)
  • A to T, chromosome 3 at 82,034,758 bp (GRCm38)
  • G to A, chromosome 3 at 88,565,878 bp (GRCm38)
  • A to G, chromosome 3 at 125,915,251 bp (GRCm38)
  • C to T, chromosome 4 at 14,546,372 bp (GRCm38)
  • A to C, chromosome 4 at 34,771,265 bp (GRCm38)
  • A to T, chromosome 4 at 98,358,024 bp (GRCm38)
  • T to A, chromosome 4 at 108,590,677 bp (GRCm38)
  • T to A, chromosome 4 at 119,626,343 bp (GRCm38)
  • T to C, chromosome 4 at 155,159,887 bp (GRCm38)
  • A to G, chromosome 5 at 71,657,120 bp (GRCm38)
  • A to G, chromosome 5 at 76,858,708 bp (GRCm38)
  • C to A, chromosome 5 at 87,843,135 bp (GRCm38)
  • A to T, chromosome 5 at 108,525,512 bp (GRCm38)
  • A to G, chromosome 5 at 123,322,470 bp (GRCm38)
  • A to G, chromosome 6 at 3,486,940 bp (GRCm38)
  • T to C, chromosome 6 at 59,354,251 bp (GRCm38)
  • T to C, chromosome 6 at 70,340,882 bp (GRCm38)
  • A to G, chromosome 6 at 102,277,235 bp (GRCm38)
  • T to C, chromosome 6 at 115,823,370 bp (GRCm38)
  • T to C, chromosome 6 at 125,144,309 bp (GRCm38)
  • T to C, chromosome 6 at 128,359,156 bp (GRCm38)
  • T to A, chromosome 6 at 140,642,295 bp (GRCm38)
  • T to C, chromosome 6 at 143,874,116 bp (GRCm38)
  • C to T, chromosome 7 at 19,398,098 bp (GRCm38)
  • T to C, chromosome 7 at 30,531,525 bp (GRCm38)
  • T to C, chromosome 7 at 45,047,530 bp (GRCm38)
  • C to T, chromosome 7 at 75,866,993 bp (GRCm38)
  • A to C, chromosome 7 at 82,763,388 bp (GRCm38)
  • T to C, chromosome 7 at 89,510,475 bp (GRCm38)
  • T to A, chromosome 8 at 18,940,733 bp (GRCm38)
  • A to T, chromosome 8 at 43,568,598 bp (GRCm38)
  • A to T, chromosome 8 at 90,986,113 bp (GRCm38)
  • A to C, chromosome 8 at 95,545,977 bp (GRCm38)
  • A to G, chromosome 8 at 106,210,891 bp (GRCm38)
  • T to A, chromosome 9 at 21,289,087 bp (GRCm38)
  • A to G, chromosome 9 at 49,456,866 bp (GRCm38)
  • T to C, chromosome 9 at 79,619,984 bp (GRCm38)
  • C to T, chromosome 9 at 98,582,848 bp (GRCm38)
  • T to C, chromosome 10 at 45,648,918 bp (GRCm38)
  • A to T, chromosome 10 at 62,480,654 bp (GRCm38)
  • T to C, chromosome 10 at 80,345,062 bp (GRCm38)
  • A to G, chromosome 10 at 88,124,953 bp (GRCm38)
  • G to T, chromosome 10 at 119,976,001 bp (GRCm38)
  • A to G, chromosome 11 at 50,815,412 bp (GRCm38)
  • T to A, chromosome 11 at 68,533,532 bp (GRCm38)
  • A to G, chromosome 11 at 72,730,575 bp (GRCm38)
  • A to G, chromosome 11 at 73,070,842 bp (GRCm38)
  • C to T, chromosome 11 at 101,055,601 bp (GRCm38)
  • G to T, chromosome 11 at 103,360,685 bp (GRCm38)
  • A to G, chromosome 11 at 114,996,949 bp (GRCm38)
  • T to C, chromosome 12 at 59,204,922 bp (GRCm38)
  • T to A, chromosome 12 at 71,944,081 bp (GRCm38)
  • C to T, chromosome 13 at 44,914,777 bp (GRCm38)
  • G to A, chromosome 13 at 55,475,011 bp (GRCm38)
  • T to A, chromosome 13 at 67,708,359 bp (GRCm38)
  • T to C, chromosome 14 at 50,959,549 bp (GRCm38)
  • T to G, chromosome 14 at 117,964,846 bp (GRCm38)
  • A to C, chromosome 15 at 55,031,044 bp (GRCm38)
  • G to A, chromosome 15 at 80,148,742 bp (GRCm38)
  • T to C, chromosome 15 at 99,944,512 bp (GRCm38)
  • C to T, chromosome 16 at 19,260,798 bp (GRCm38)
  • C to A, chromosome 16 at 31,110,945 bp (GRCm38)
  • T to A, chromosome 16 at 44,909,057 bp (GRCm38)
  • A to T, chromosome 16 at 48,928,344 bp (GRCm38)
  • T to A, chromosome 16 at 89,419,875 bp (GRCm38)
  • A to T, chromosome 17 at 24,721,836 bp (GRCm38)
  • A to T, chromosome 17 at 27,502,714 bp (GRCm38)
  • T to C, chromosome 17 at 66,366,352 bp (GRCm38)
  • C to A, chromosome 18 at 37,854,536 bp (GRCm38)
  • A to T, chromosome 18 at 80,449,335 bp (GRCm38)
  • G to C, chromosome 19 at 12,428,933 bp (GRCm38)
  • G to A, chromosome 19 at 47,070,995 bp (GRCm38)
  • G to T, chromosome 19 at 57,037,259 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9762 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
070007-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.