Strain Detail Sheet

Strain Name:
Nagy ES cell line G4 with Z/red transgene
Stock Number:
011987-MU
Citation ID:
RRID:MMRRC_011987-MU
Major Collection:
Nagy Basic ES Cell lines

Gene Information

Gene Details

lacZ
Name: beta galactosidase
Type: Gene
Species: Escherichia coli (bacterium)
Chromosome:
ACTB
Name: actin, beta
Type: Gene
Species: Gallus gallus (chicken)
Chromosome: 14
CMV
Name: cytomegalovirus
Type: Regulatory Region
Species: Virus
Chromosome:
Alteration at locus: Transgenic
MGI:
Genetic Alterations
The cell line has LacZ expression under control of a chicken beta actin promoter coupled with CMV immediate early enhancer. The beta-galactosidase gene is flanked by lox P sites and in the presence of cre-recombinase the beta-galactoside is excised and this allows expression of the second reporter contained in this cell line which is DsRed.MST (variant of DsRed).
Genotype Determination
ES Cell Line
G4 derived from (129S6/SvEvTac x C57BL/6Ncr)F1

Related Protocols

Strain Origin

Donor
Andras Nagy, Ph.D., Mt. Sinai Hospital, Toronto
Primary Reference
  • Vintersten K, Monetti C, Gertsenstein M, Zhang P, Laszlo L, Biechele S, Nagy A. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis. 2004 Dec;40(4):241-6. Erratum in: Genesis. 2005 Jul;42(3):218. (Medline PMID: 15593332)
  • George SH, Gertsenstein M, Vintersten K, Korets-Smith E, Murphy J, Stevens ME, Haigh JJ, Nagy A. Developmental and adult phenotyping directly from mutant embryonic stem cells. Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4455-60. Epub 2007 Mar 2. (Medline PMID: 17360545)

Colony and Husbandry Information

Special Considerations

Health Status Report

This ES cell line has been tested and found to be free of Mycoplasma sp., MPV and MVM. Please contact MU Technical Services at mmrrc@missouri.edu or at (888) 673-6459 if you would like to order additional health testing.

Order Request Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
011987-MU-CELL Nagy ES cell line G4 with Z/red transgene $546.00 / Non-Profit Vial Includes genetic confirmation
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.