Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5069Btlr/Mmmh
Stock Number:
042659-MU
Citation ID:
RRID:MMRRC_042659-MU
Other Names:
R5069 (G1), C57BL/6J-MtgxR5069Btlr
Major Collection:

Strain Information

Pald1
Name: phosphatase domain containing, paladin 1
Synonyms: paladin, X99384
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27355
VEGA: 10
Homologene: 8453
Cdh3
Name: cadherin 3
Synonyms: P-cadherin, Cadp, Pcad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12560
HGNC: HGNC:1762
Homologene: 20425
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Nhlh1
Name: nescient helix loop helix 1
Synonyms: Hen1, Nscl, Tal2, Nscl, bHLHa35
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18071
HGNC: HGNC:7817
Homologene: 4089
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 38,181,613 bp
  • T to C, chromosome 1 at 72,680,210 bp
  • G to A, chromosome 1 at 74,385,184 bp
  • A to T, chromosome 1 at 86,257,237 bp
  • T to A, chromosome 1 at 92,573,413 bp
  • T to C, chromosome 1 at 163,987,674 bp
  • A to G, chromosome 1 at 172,053,900 bp
  • C to T, chromosome 2 at 31,510,173 bp
  • G to A, chromosome 2 at 62,267,571 bp
  • A to T, chromosome 2 at 122,494,373 bp
  • G to T, chromosome 2 at 180,984,573 bp
  • G to A, chromosome 2 at 181,355,492 bp
  • A to G, chromosome 3 at 99,187,533 bp
  • T to A, chromosome 3 at 107,104,637 bp
  • A to G, chromosome 3 at 152,094,248 bp
  • T to A, chromosome 4 at 123,817,917 bp
  • T to A, chromosome 4 at 144,703,867 bp
  • T to A, chromosome 4 at 152,279,883 bp
  • T to A, chromosome 5 at 9,440,897 bp
  • T to G, chromosome 5 at 37,187,236 bp
  • T to C, chromosome 5 at 64,926,400 bp
  • A to G, chromosome 5 at 139,248,548 bp
  • C to T, chromosome 6 at 7,982,713 bp
  • A to T, chromosome 6 at 42,038,417 bp
  • A to T, chromosome 6 at 115,965,901 bp
  • A to G, chromosome 6 at 124,413,596 bp
  • A to T, chromosome 7 at 35,628,435 bp
  • T to G, chromosome 7 at 45,310,469 bp
  • T to C, chromosome 7 at 102,145,655 bp
  • A to T, chromosome 7 at 103,338,022 bp
  • T to C, chromosome 7 at 120,032,790 bp
  • A to G, chromosome 7 at 128,676,727 bp
  • A to G, chromosome 7 at 139,678,507 bp
  • A to G, chromosome 7 at 141,651,299 bp
  • G to A, chromosome 7 at 144,514,473 bp
  • G to A, chromosome 8 at 22,903,133 bp
  • G to A, chromosome 8 at 25,593,964 bp
  • A to G, chromosome 8 at 53,601,041 bp
  • A to G, chromosome 8 at 63,355,265 bp
  • T to C, chromosome 8 at 88,327,697 bp
  • A to G, chromosome 8 at 106,536,826 bp
  • T to C, chromosome 9 at 44,940,089 bp
  • A to T, chromosome 9 at 45,939,461 bp
  • A to T, chromosome 9 at 56,926,191 bp
  • T to A, chromosome 10 at 21,354,647 bp
  • T to G, chromosome 10 at 24,000,461 bp
  • A to T, chromosome 10 at 61,341,246 bp
  • A to T, chromosome 10 at 88,886,598 bp
  • A to C, chromosome 10 at 92,937,774 bp
  • T to A, chromosome 10 at 115,151,174 bp
  • T to A, chromosome 10 at 115,418,783 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • T to C, chromosome 11 at 69,736,735 bp
  • A to G, chromosome 13 at 12,288,522 bp
  • G to A, chromosome 13 at 14,616,028 bp
  • A to C, chromosome 13 at 38,197,123 bp
  • A to C, chromosome 13 at 46,709,792 bp
  • T to C, chromosome 13 at 49,717,899 bp
  • T to C, chromosome 13 at 98,075,206 bp
  • T to C, chromosome 14 at 32,570,063 bp
  • A to G, chromosome 14 at 32,661,792 bp
  • T to A, chromosome 14 at 50,618,740 bp
  • C to A, chromosome 14 at 50,737,074 bp
  • T to A, chromosome 14 at 54,439,113 bp
  • T to A, chromosome 14 at 54,986,854 bp
  • C to T, chromosome 14 at 66,308,972 bp
  • T to C, chromosome 15 at 54,864,054 bp
  • A to G, chromosome 15 at 101,566,238 bp
  • A to G, chromosome 15 at 102,711,092 bp
  • A to G, chromosome 16 at 18,398,986 bp
  • G to T, chromosome 16 at 31,684,295 bp
  • A to T, chromosome 16 at 37,631,855 bp
  • A to T, chromosome 17 at 25,249,108 bp
  • A to G, chromosome 17 at 31,501,284 bp
  • G to A, chromosome 17 at 35,024,190 bp
  • A to G, chromosome 17 at 37,063,389 bp
  • C to T, chromosome 17 at 74,565,972 bp
  • T to A, chromosome 17 at 74,799,342 bp
  • A to G, chromosome 18 at 10,793,002 bp
  • A to G, chromosome 18 at 77,563,310 bp
  • C to T, chromosome 19 at 4,111,140 bp
  • A to G, chromosome 19 at 10,439,237 bp
  • A to G, chromosome 19 at 33,619,709 bp
  • A to T, chromosome 19 at 37,905,325 bp
  • T to A, chromosome 19 at 44,935,253 bp
  • C to T, chromosome X at 41,806,693 bp
  • T to C, chromosome X at 73,127,971 bp
  • T to A, chromosome X at 73,238,684 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5069 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042659-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.