Loading...

Strain Detail Sheet

Strain Name:
B6.129P2-Gabrqtm1Dgen/Mmnc
Stock Number:
011620-UNC
Citation ID:
RRID:MMRRC_011620-UNC
Major Collection:
Deltagen

Strain Information

Gene Details

Gabrqtm1Dgen
Name: gamma-aminobutyric acid type A receptor subunit theta; targeted mutation 1, Deltagen
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
Gabrq
Name: gamma-aminobutyric acid type A receptor subunit theta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
NCBI: 57249
Homologene: 10233

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
The endogenous gene promoter drives expression of inserted lacZ gene.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    E14 derived from 129P2/OlaHsd

    Strain Description

    Phenotype
    Behavior: Hemizygous mutant mice exhibited significantly decreased prepulse inhibition when compared with age- and gender-matched wild-type control mice.
    There were no other significant differences detected in the female homozygous or male hemizygous mutant mice when compared with age- and gender-matched heterozygous or wild-type control mice.

    As this gene is X-linked, the cohort consists of homozygous mutant females, hemizygous mutant males (-/0), heterozygous control females, and wild-type control males (+/0). No wild-type females or heterozygous males were analyzed for this target, as such mice cannot be generated from a male -/0 x female -/+ mating.

    Phenotypic data provided by Deltagen, Inc. Full report available through MGI data link (includes genotyping information under "Molecular Biology" at top of screen).
    Strain Development
    ES cells derived from the 129/OlaHsd mouse substrain were used to generate chimeric mice. F1 mice were generated by breeding with C57BL/6 females. The resultant F1N0 heterozygotes were backcrossed to C57BL/6 mice to generate F1N1 heterozygotes. F2N1 homozygous mutant mice were produced by intercrossing F1N1 heterozygous males and females. Subsequent crosses made to C57BL/6.

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on two XY samples (TU1546, EJ9304) at the time of importation and the conclusions are consistent in all samples. The results of the analysis are fully congruent with the SDS and EJ9304 is the representative sample selected for the report. The primary (host) background is C57BL/6. There is positive evidence for the presence of the C57BL/6J and C57BL/6NCrl substrains. The secondary (donor) background is consistent with 129P2/OlaHsd, which is indicated in the submission and the SDS as the donor strain. The mitochondrial genome is consistent with both the host and donor backgrounds (identical by descent). The Y chromosome is consistent with at least one backcross to a male from the host background. The region containing the allele of interest has the expected donor background (129P2/OlaHsd). Both samples are hemizygous for the donor background at the locus. The following construct(s) are detected in all samples: IRES.

    The genome of MMRRC:011620 mice is 98.6 % inbred and 99.5% congenic at the strain level. 1.4% of the genome is segregating for the secondary (donor) background. 71.9% of the genome is segregating for the two substrains (C57BL/6J and C57BL/6NCrl) that contribute to the primary (host) background. The contribution of C57BL/6J is 60.4%. The contribution of C57BL/6NCrl is 39.6%.

    The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible.
    MiniMUGA Sample Report
    MiniMUGA Sample Report Link

    Strain Origin

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires execution of the Deltagen Standard Academic license agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    011620-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.