Strain Name    : B6.129P2-Gria4tm1Dgen/Mmnc

Stock Number : 030968-UNC

Gene Information

(provided by MGI)
Allele Symbol: Gria4tm1Dgen
Name: targeted mutation 1, Deltagen
Chromosome: 9
Alteration at locus: Knockout
Gene Symbol: Gria4
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)
Chromosome: 9
Alteration at locus: Knockout
Genetic Alterations:
Insertion into locus.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line: Not specified derived from B6;129P2

    Phenotype

    Homozygous phenotype: absence seizures

    Heterozygous phenotype: none obvious

    Mammalian Phenotype Terms:
    Allelic Composition: Gria4tm1Dgen/Gria4tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    • behavior/neurological
    • nervous system
      • abnormal seizure response to pharmacological agent [MP:0000950]
    • integument
      • increased thermal nociceptive threshold [MP:0001973]
    Allelic Composition: Gria4tm1Dgen/Gria4tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

    Allelic Composition: Gria4tm1Dgen/Gria4+ (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

    Allelic Composition: Gria4spkw1/Gria4tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C3H/HeJ * C57BL/6J )

    Strain of Origin: 129P2/OlaHsd

    Strain genetic background: C57BL/6J

    Strain Development: Donor originally obtained the Gria4 knockout made by Deltagen (MMRRC:011625) and has since backcrossed it 10+ generations to C57BL/6J.

    Suggested Control Mice:

    • Wildtype littermates

    Donor: Wayne Frankel, Ph.D., The Jackson Laboratory

    Primary Reference:

    Beyer B; Deleuze C; Letts VA; Mahaffey CL; Boumil RM; Lew TA; Huguenard JR; Frankel WN, Absence seizures in C3H/HeJ and knockout mice caused by mutation of the AMPA receptor subunit Gria4., Hum Mol Genet 2008 Jun 15;17(12):1738-49 (Medline PMID: 18316356)

    Colony and Husbandry Information

    Occasionally homozygotes maybe smaller and slightly ataxic - this phenotype is not fully penetrant.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires execution of the Deltagen Standard Academic license agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.The donor or their institution limits the distribution to non-profit institutions only.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    030968-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.