Allele Symbol: Gria3tm1Dgen
Name: targeted mutation 1, Deltagen
Alteration at locus: Knockout
Gene Symbol: Gria3
Name: glutamate receptor, ionotropic, AMPA3 (alpha 3)
Alteration at locus: Knockout
ES Cell Line: Not specified derived from B6;129P2
Homozygous phenotype: none obvious; together with Gria4 mutation it affects the incidence of absence seizures.
Hemizygous phenotype: none obvious
Strain of Origin: 129P2/OlaHsd
Strain genetic background: C57BL/6J
Strain Development: This mutation, a Deltagen knockout, was originally obtained from the MMRRC on a mixed background, MMRRC:011624, B6;129P2-Gria3tm1Dgen/Mmnc. The donor has since backcrossed it to C57BL/6J an additional 10 generations to make it congenic.
Suggested Control Mice:
Donor: Wayne Frankel, Ph.D., The Jackson Laboratory
Beyer B, Deleuze C, Letts VA, Mahaffey CL, Boumil RM, Lew TA, Huguenard JR, Frankel WN. Absence seizures in C3H/HeJ and knockout mice caused by mutation of the AMPA receptor subunit Gria4. Hum Mol Genet. 2008 Jun 15;17(12):1738-49. Epub 2008 Mar 3. (Medline PMID: 18316356 )
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.