Strain Detail Sheet

Strain Name    :

B6.129P2-Gria3tm1Dgen/Mmnc

Stock Number :

030969-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Gria3tm1Dgen
Name: targeted mutation 1, Deltagen
Alteration at locus: Knockout
Gene Symbol: Gria3
Name: glutamate receptor, ionotropic, AMPA3 (alpha 3)
Chromosome: X
Alteration at locus: Knockout

Genetic Alterations:
Deltagen knockout - exon replacement - in the Gria3 gene

Genotype Determination:

ES Cell Line: Not specified derived from B6;129P2

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: none obvious; together with Gria4 mutation it affects the incidence of absence seizures.

Hemizygous phenotype: none obvious


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Gria3tm1Dgen/Gria3tm1Dgen
        involves: 129P2/OlaHsd * C57BL/6
  • growth/size phenotype
    • decreased body weight (MGI Ref ID J:101679)
      • at 300 days of age body weight, body mass, and/or body weight to length ratios are reduced
Gria3tm1Dgen/Gria3tm1Dgen
        involves: 129P2/OlaHsd * C57BL/6J
  • behavior/neurological phenotype
    • behavior/neurological phenotype (MGI Ref ID J:135814)
      • mice show no spike wave discharge (SWD)
      • with age, no ataxia is observed
Gria3tm1Dgen/-
        involves: 129P2/OlaHsd * C57BL/6
  • behavior/neurological phenotype
    • decreased startle reflex (MGI Ref ID J:101679)
      • startle response is decreased in response to high intensity sound stimuli
    • decreased susceptibility to pharmacologically induced seizures (MGI Ref ID J:101679)
      • a higher dose of metrazol is needed to induce a first twitch stage of seizure response compared to wild-type mice
    • increased thermal nociceptive threshold (MGI Ref ID J:101679)
      • prolonged latency in hot plate test
  • hearing/vestibular/ear phenotype
    • decreased startle reflex (MGI Ref ID J:101679)
      • startle response is decreased in response to high intensity sound stimuli
  • nervous system phenotype
    • decreased susceptibility to pharmacologically induced seizures (MGI Ref ID J:101679)
      • a higher dose of metrazol is needed to induce a first twitch stage of seizure response compared to wild-type mice
  • integument phenotype
    • increased thermal nociceptive threshold (MGI Ref ID J:101679)
      • prolonged latency in hot plate test

Strain of Origin: 129P2/OlaHsd

Strain genetic background: C57BL/6J

Strain Development: This mutation, a Deltagen knockout, was originally obtained from the MMRRC on a mixed background, MMRRC:011624, B6;129P2-Gria3tm1Dgen/Mmnc. The donor has since backcrossed it to C57BL/6J an additional 10 generations to make it congenic.

Suggested Control Mice:

  • Wild-type littermates

Research Applications

  • Neurobiology

Strain Origin

Donor: Wayne Frankel, Ph.D., The Jackson Laboratory

Primary Reference:

Beyer B, Deleuze C, Letts VA, Mahaffey CL, Boumil RM, Lew TA, Huguenard JR, Frankel WN. Absence seizures in C3H/HeJ and knockout mice caused by mutation of the AMPA receptor subunit Gria4. Hum Mol Genet. 2008 Jun 15;17(12):1738-49. Epub 2008 Mar 3. (Medline PMID: 18316356 )

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires execution of the Deltagen Standard Academic license agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030969-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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