Strain Name:
B6.129P2-Gria3tm1Dgen/Mmnc
Stock Number:
030969-UNC
Citation ID:
RRID:MMRRC_030969-UNC
Major Collection:

Strain Information

Gria3tm1Dgen
Name: glutamate receptor, ionotropic, AMPA3 (alpha 3); targeted mutation 1, Deltagen
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
Gria3
Name: glutamate receptor, ionotropic, AMPA3 (alpha 3)
Synonyms: Glur-3, Glur3, GluR3, 2900064I19Rik, Gluralpha3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
NCBI: 53623
HGNC: HGNC:4573
Homologene: 37353
Genetic Alterations
Deltagen knockout - exon replacement - in the Gria3 gene
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype
    Homozygous: same as wild-type; together with Gria4 mutation it affects the incidence of absence seizures.

    Hemizygous: same as wild-type
    Mammalian Phenotype Terms
    Allelic Composition: Gria3tm1Dgen/Gria3tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    • growth/size/body
    Allelic Composition: Gria3tm1Dgen/Gria3tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

    MeSH Terms
    • Animals
    • Electroencephalography
    • Epilepsy, Absence/genetics
    • Epilepsy, Absence/physiopathology
    • Mice
    • Mice, Inbred C3H
    • Mice, Knockout
    • Molecular Sequence Data
    • Receptors, AMPA/genetics
    • Receptors, AMPA/metabolism
    • Synapses/physiology
    • Thalamus/physiology
    Strain Development
    This mutation, a Deltagen knockout, was originally obtained from the MMRRC on a mixed background, MMRRC:011624, B6;129P2-Gria3tm1Dgen/Mmnc. The donor has since backcrossed it to C57BL/6J an additional 10 generations to make it congenic.
    Suggested Control Mice

    Wild-type littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    Donor
    Wayne Frankel, Ph.D., The Jackson Laboratory
    Primary Reference
    Beyer B, Deleuze C, Letts VA, Mahaffey CL, Boumil RM, Lew TA, Huguenard JR, Frankel WN. Absence seizures in C3H/HeJ and knockout mice caused by mutation of the AMPA receptor subunit Gria4. Hum Mol Genet. 2008 Jun 15;17(12):1738-49. Epub 2008 Mar 3. (Medline PMID: 18316356 )

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires execution of the Deltagen Standard Academic license agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    030969-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.