Strain Name:
B6.129P2-Rdh9tm1Jln/Mmucd
Stock Number:
036649-UCD
Citation ID:
RRID:MMRRC_036649-UCD
Other Names:
Crad3 KO, Rdh9 KO

Gene Information

Allele Symbol: Rdh9tm1Jln (Mus musculus (mouse))
Name: retinol dehydrogenase 9; targeted mutation 1, Joseph L Napoli
Synonyms: Rdh9<->
Chromosome: 10
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:3711415
Gene Symbol: Rdh9 (Mus musculus (mouse))
Name: retinol dehydrogenase 9
Synonyms: Crad3
Chromosome: 10
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:2143528
Genetic Alterations
Exon 1 was flanked by loxP sites. Transient expression of cre recombinase removed the floxed sequence. RT-PCR confirmed absence of transcript in mutant liver.
Genotype Determination
ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype

Homozygous: no abnormal phenotype detected.

Heterozygous: no abnormal phenotype detected.

Mammalian Phenotype Terms
Allelic Composition: Rdh9tm1Jln/Rdh9tm1Jln (Genetic Background: B6.129P2-Rdh9tm1Jln )

MeSH Terms
  • Alcohol Oxidoreductases/deficiency
  • Alcohol Oxidoreductases/genetics
  • Alcohol Oxidoreductases/metabolism
  • Androgens/metabolism
  • Animals
  • Cytochrome P-450 Enzyme System/metabolism
  • Embryo, Mammalian/enzymology
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Growth and Development/genetics
  • Hydroxysteroid Dehydrogenases/metabolism
  • In Situ Hybridization
  • Isoenzymes
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger/metabolism
  • Retinoid X Receptors/metabolism
  • Retinoids/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Steroids/metabolism
  • Time Factors
Strain Development
ES cells were injected into a C57BL/6J blastocyst. The knockout was then backcrossed 5 times into C57BL6/J mice. Homozygous knockout mice were then interbred for approximately a year. Mice were then backcrossed a sixth time into C57BL/6J. Homozygous knockout mice were once again generated and interbred.
Suggested Control Mice
Wild-type littermates
  • Endocrine Deficiency
Donor
Joseph Napoli, Ph.D., University of California, Berkeley.
Primary Reference

Hu P, Zhang M, Napoli JL. Ontogeny of rdh9 (Crad3) expression: ablation causes changes in retinoid and steroid metabolizing enzymes, but RXR and androgen signaling seem normal. Biochim Biophys Acta. 2007 Apr;1770(4):694-705. Epub 2006 Dec 24. (Medline PMID: 17270348). 

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Backcross or Random intra-strain mating
Breeding Scheme(s)
  • C57BL/6J females x Heterozygous male or recipracol mating
  • Heterozygous females x Heterozygous male
  • Homozygous females x Homozgyous male
Generation
N6
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036649-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,549.67
Non-Profit / For-Profit
Aliquot Approximate quantity3 : 20-40 embryos / aliquot
036649-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $871.90
Non-Profit / For-Profit
Aliquot Approximate quantity2
036649-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $6,017.01
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.