The Tcrα knockout/transgenic LCMV P14 T cell receptor line is homozygous for a transgene that encodes a T cell receptor that is specific for a peptide (P14) from the lymphocytic choriomeningitis virus (LCMV) presented by the MHC class I molecule H-2Db. The transgene carries both Tcra and Tcrb. Ten to twenty copies of the Tcr transgene were reported to have integrated on the same chromosome. At least two transgenic lines are known to exist. Line 327 is the representative line. The second line is 318. This line is also deficient in the Tcrα gene and, therefore, does not develop endogenous mature TCR alpha beta cells.
The transgenic LCMV-P14 T cell receptor transgenic mouse was made by Dr. Hanspeter Pircher and colleagues in 1989 at the Department of Experimental Pathology in Zurich, Switzerland. The transgenic line was created by coinjection of the P14 Tcrα and Tcrβ gene constructs into (C57BL/6 x DBA/2)F2-fertilized eggs. The male founder 327, who had 10-20 copies of both transgenes integrated onto the same chromosome, was crossed to a C57BL/6 female. The line was subsequently backcrossed to C57BL/6 up to N10 and then intracrossed to establish a homozygous line. These mice were brought to Taconic in May of 1997 by Dr. B.J. Fowlkes and embryo transfer-derived into the NIAID colony. In 1998, this strain was crossed twice to TCRa-targeted mutation mice (B6.129S2-Tcratm1Mom N12). The Tcra knockout line was developed in the laboratory of Dr. S. Tonegawa by Dr. P. Mombaerts in 1992 by targeting in 129S2/SvPas-derived D3 ES cells and subsequently backcrossed to C57BL/6 12 times. After the second cross to the Tcra-/- strain, mice homozygous for the Tcra mutation were selected by looking for the disappearance of endogenous CD4+ T cells using FACS analysis. The line was then bred to obtain mice homozygous for the transgene followed by selection using test crosses and FACS analysis to identify mice exhibiting high levels of expression of the Vβ 8.1, Vα 2 transgene.
The line was donated to the MMRRC in 2014.
Pircher H, Burki K, Lang R, Hengartner H, Zinkernagel RM. Tolerance induction in double specific T-cell receptor transgenic mice varies with antigen. Nature. 1989 Nov 30;342(6249):559-61. (Medline PMID: 2573841)Mombaerts P, Clarke AR, Rudnicki MA, Iacomini J, Itohara S, Lafaille JJ, Wang L, Ichikawa Y, Jaenisch R, Hooper ML, et al. Mutations in T-cell antigen receptor genes alpha and beta block thymocyte development at different stages. Nature. 1992 Nov 19;360(6401):225-31. (Medline PMID: 1359428)
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Unknown||Unknown|
|Hetero/Hemizygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Undetermined||Undetermined|
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to firstname.lastname@example.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|MMRRC Item #||Description||Distribution Fee / Unit (US $)||Units||Notes|
|037394-JAX-SPERM||Cryo-preserved spermatozoa||$437.00 / Non-Profit||Aliquot||Approximate quantity3|
|037394-JAX-RESUS||Litter recovered from cryo-archive||$2,022.00 / Non-Profit||Litter||Recovered litter4; additional fees for any special requests.|
|Cryopreserved material may be available upon request, please inquire to email@example.com for more information.|
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.