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Strain Name:
C57BL/6J-MtgxR0027Btlr/Mmmh
Stock Number:
038322-MU
Citation ID:
RRID:MMRRC_038322-MU
Other Names:
R0027 (G1), C57BL/6J-MtgxR0027Btlr
Major Collection:

Strain Information

Rest
Name: RE1-silencing transcription factor
Synonyms: 2610008J04Rik, NRSF
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19712
HGNC: HGNC:9966
Homologene: 4099
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Hspa4
Name: heat shock protein 4
Synonyms: APG-2, Hsp70RY, Hsp110, 70kDa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15525
HGNC: HGNC:5237
Homologene: 1624
Kif11
Name: kinesin family member 11
Synonyms: Eg5, Knsl1, Kifl1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16551
VEGA: 19
HGNC: HGNC:6388
Homologene: 3322
Setx
Name: senataxin
Synonyms: A930037J23Rik, Als4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269254
HGNC: HGNC:445
Homologene: 41003
Gse1
Name: genetic suppressor element 1, coiled-coil protein
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382034
Homologene: 40964
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 34,189,119 bp
  • T to C, chromosome 1 at 86,094,265 bp
  • T to C, chromosome 1 at 153,262,583 bp
  • T to G, chromosome 2 at 29,139,221 bp
  • G to A, chromosome 2 at 82,257,200 bp
  • T to A, chromosome 2 at 91,152,549 bp
  • G to A, chromosome 2 at 102,400,247 bp
  • A to T, chromosome 2 at 103,775,580 bp
  • A to T, chromosome 2 at 104,255,044 bp
  • A to T, chromosome 2 at 120,703,501 bp
  • G to T, chromosome 2 at 128,641,511 bp
  • TA to TANA, chromosome 3 at 53,845,256 bp
  • A to G, chromosome 3 at 89,159,825 bp
  • A to G, chromosome 3 at 95,302,322 bp
  • A to G, chromosome 3 at 102,895,910 bp
  • A to G, chromosome 3 at 123,671,513 bp
  • A to G, chromosome 4 at 32,643,810 bp
  • C to T, chromosome 4 at 94,556,790 bp
  • T to C, chromosome 4 at 104,704,199 bp
  • T to A, chromosome 4 at 130,368,273 bp
  • C to A, chromosome 4 at 139,400,393 bp
  • T to C, chromosome 5 at 77,282,551 bp
  • A to G, chromosome 5 at 122,351,723 bp
  • G to A, chromosome 5 at 130,152,573 bp
  • T to C, chromosome 5 at 137,406,519 bp
  • A to T, chromosome 5 at 142,450,340 bp
  • G to A, chromosome 5 at 143,308,062 bp
  • A to T, chromosome 5 at 144,989,697 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • T to A, chromosome 6 at 42,318,248 bp
  • T to C, chromosome 6 at 116,556,949 bp
  • T to C, chromosome 7 at 5,322,448 bp
  • T to A, chromosome 7 at 25,287,140 bp
  • C to A, chromosome 7 at 25,287,141 bp
  • T to A, chromosome 7 at 28,881,960 bp
  • T to C, chromosome 7 at 63,891,761 bp
  • T to C, chromosome 8 at 120,566,546 bp
  • C to A, chromosome 9 at 110,379,730 bp
  • A to G, chromosome 10 at 61,850,161 bp
  • G to A, chromosome 10 at 83,364,529 bp
  • G to A, chromosome 10 at 123,036,048 bp
  • C to T, chromosome 11 at 52,032,349 bp
  • T to A, chromosome 11 at 53,283,585 bp
  • C to A, chromosome 11 at 78,488,091 bp
  • T to A, chromosome 11 at 80,193,942 bp
  • T to C, chromosome 11 at 93,924,549 bp
  • A to G, chromosome 11 at 106,376,276 bp
  • C to T, chromosome 12 at 101,885,169 bp
  • A to T, chromosome 12 at 105,191,239 bp
  • A to C, chromosome 12 at 105,833,136 bp
  • T to C, chromosome 12 at 108,536,298 bp
  • T to A, chromosome 13 at 27,318,028 bp
  • T to A, chromosome 13 at 97,945,696 bp
  • T to A, chromosome 14 at 55,494,403 bp
  • T to A, chromosome 14 at 93,888,645 bp
  • G to T, chromosome 14 at 118,235,617 bp
  • T to C, chromosome 14 at 122,476,343 bp
  • A to T, chromosome 15 at 11,285,873 bp
  • C to T, chromosome 17 at 28,164,357 bp
  • T to C, chromosome 17 at 48,264,934 bp
  • G to A, chromosome 17 at 84,767,007 bp
  • A to T, chromosome 18 at 49,957,295 bp
  • C to T, chromosome 19 at 37,406,983 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0027 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038322-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.