Strain Name:
C57BL/6J-MtgxR0049Btlr/Mmmh
Stock Number:
038343-MU
Citation ID:
RRID:MMRRC_038343-MU
Other Names:
R0049 (G1), C57BL/6J-MtgxR0049Btlr
Major Collection:

Gene Information

Gene Symbol: Kif3a [MGI:107689] (Mus musculus (mouse))
Name: kinesin family member 3A
Synonyms: Kifl, Kif3, N-4 kinesin, kinesin-II subunit, Kns3
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Kif3c [MGI:107979] (Mus musculus (mouse))
Name: kinesin family member 3C
Synonyms: N-4 kinesin
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Pax3 [MGI:97487] (Mus musculus (mouse))
Name: paired box 3
Synonyms: Pax-3, Splchl2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Gga3 [MGI:2384159] (Mus musculus (mouse))
Name: golgi associated, gamma adaptin ear containing, ARF binding protein 3
Synonyms: C230037M19Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ngf [MGI:97321] (Mus musculus (mouse))
Name: nerve growth factor
Synonyms: Ngfb
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Satb1 [MGI:105084] (Mus musculus (mouse))
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Adgre1 [MGI:106912] (Mus musculus (mouse))
Name: adhesion G protein-coupled receptor E1
Synonyms: TM7LN3, EGF-TM7, DD7A5-7, F4/80, Emr1, Ly71
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,275,781 bp
  • G to T, chromosome 1 at 60,525,899 bp
  • A to G, chromosome 1 at 78,103,504 bp
  • C to A, chromosome 1 at 87,834,618 bp
  • T to C, chromosome 1 at 91,196,870 bp
  • A to T, chromosome 1 at 156,301,903 bp
  • T to A, chromosome 1 at 171,214,413 bp
  • G to A, chromosome 2 at 11,283,832 bp
  • G to C, chromosome 2 at 28,542,379 bp
  • T to A, chromosome 2 at 32,252,011 bp
  • A to C, chromosome 2 at 52,170,467 bp
  • A to G, chromosome 2 at 66,223,564 bp
  • T to C, chromosome 2 at 70,690,723 bp
  • T to C, chromosome 2 at 91,670,169 bp
  • A to G, chromosome 2 at 114,523,044 bp
  • C to A, chromosome 2 at 120,699,819 bp
  • C to T, chromosome 2 at 121,519,451 bp
  • T to A, chromosome 2 at 122,296,686 bp
  • G to A, chromosome 2 at 179,924,091 bp
  • T to A, chromosome 3 at 30,607,081 bp
  • T to A, chromosome 3 at 87,729,193 bp
  • T to C, chromosome 3 at 89,371,606 bp
  • A to T, chromosome 3 at 102,520,345 bp
  • T to A, chromosome 3 at 108,397,254 bp
  • T to A, chromosome 3 at 108,953,789 bp
  • C to A, chromosome 3 at 154,611,552 bp
  • A to T, chromosome 4 at 43,036,441 bp
  • G to A, chromosome 4 at 45,930,119 bp
  • A to G, chromosome 4 at 45,987,582 bp
  • T to A, chromosome 4 at 63,394,635 bp
  • T to C, chromosome 5 at 5,613,734 bp
  • T to A, chromosome 5 at 8,825,661 bp
  • T to A, chromosome 5 at 21,375,663 bp
  • A to C, chromosome 5 at 34,908,662 bp
  • T to G, chromosome 5 at 37,265,273 bp
  • T to A, chromosome 5 at 96,776,622 bp
  • C to T, chromosome 5 at 104,302,158 bp
  • C to A, chromosome 5 at 120,795,330 bp
  • T to C, chromosome 5 at 127,663,327 bp
  • T to C, chromosome 5 at 138,251,304 bp
  • A to T, chromosome 6 at 28,421,269 bp
  • G to C, chromosome 6 at 33,296,922 bp
  • A to G, chromosome 6 at 35,051,277 bp
  • G to A, chromosome 6 at 38,082,081 bp
  • A to T, chromosome 6 at 121,638,308 bp
  • T to A, chromosome 6 at 124,459,853 bp
  • T to C, chromosome 6 at 141,990,421 bp
  • T to C, chromosome 6 at 146,650,234 bp
  • T to A, chromosome 7 at 4,573,362 bp
  • G to T, chromosome 7 at 6,711,673 bp
  • G to T, chromosome 7 at 18,889,388 bp
  • T to C, chromosome 7 at 28,861,777 bp
  • A to G, chromosome 7 at 36,770,109 bp
  • G to A, chromosome 7 at 86,148,101 bp
  • C to T, chromosome 7 at 104,429,352 bp
  • G to A, chromosome 7 at 126,866,411 bp
  • A to T, chromosome 7 at 127,024,586 bp
  • C to A, chromosome 8 at 3,598,772 bp
  • A to C, chromosome 8 at 14,954,446 bp
  • A to T, chromosome 8 at 45,942,626 bp
  • A to G, chromosome 8 at 70,391,859 bp
  • T to A, chromosome 8 at 111,052,451 bp
  • A to T, chromosome 8 at 123,827,342 bp
  • T to C, chromosome 9 at 58,860,299 bp
  • T to C, chromosome 9 at 105,055,673 bp
  • T to A, chromosome 9 at 123,855,875 bp
  • T to A, chromosome 10 at 20,348,412 bp
  • C to A, chromosome 10 at 22,180,862 bp
  • A to G, chromosome 10 at 43,921,434 bp
  • T to A, chromosome 10 at 52,101,761 bp
  • G to T, chromosome 10 at 76,369,821 bp
  • A to G, chromosome 10 at 79,931,065 bp
  • A to G, chromosome 10 at 85,885,616 bp
  • A to G, chromosome 10 at 108,253,332 bp
  • A to T, chromosome 11 at 29,246,514 bp
  • A to C, chromosome 11 at 32,499,223 bp
  • G to T, chromosome 11 at 32,499,224 bp
  • T to G, chromosome 11 at 42,593,847 bp
  • T to A, chromosome 11 at 53,590,733 bp
  • T to C, chromosome 11 at 59,766,745 bp
  • C to T, chromosome 11 at 67,099,672 bp
  • C to A, chromosome 11 at 88,839,624 bp
  • C to A, chromosome 11 at 115,587,089 bp
  • A to C, chromosome 12 at 3,367,090 bp
  • A to T, chromosome 12 at 4,748,117 bp
  • C to T, chromosome 13 at 23,137,055 bp
  • A to T, chromosome 13 at 27,317,997 bp
  • A to T, chromosome 13 at 49,524,446 bp
  • T to C, chromosome 13 at 55,426,317 bp
  • A to T, chromosome 13 at 58,303,564 bp
  • T to C, chromosome 14 at 20,462,610 bp
  • A to T, chromosome 14 at 50,533,694 bp
  • T to A, chromosome 14 at 50,959,533 bp
  • T to A, chromosome 14 at 52,428,698 bp
  • T to A, chromosome 14 at 79,093,739 bp
  • T to C, chromosome 14 at 79,565,525 bp
  • T to A, chromosome 14 at 103,055,207 bp
  • A to G, chromosome 15 at 75,709,239 bp
  • T to C, chromosome 15 at 77,959,724 bp
  • T to A, chromosome 15 at 79,727,609 bp
  • T to C, chromosome 15 at 81,694,012 bp
  • T to C, chromosome 15 at 98,565,079 bp
  • G to T, chromosome 16 at 23,321,141 bp
  • G to T, chromosome 16 at 23,612,929 bp
  • A to T, chromosome 16 at 32,298,469 bp
  • T to A, chromosome 17 at 34,709,568 bp
  • T to A, chromosome 17 at 37,624,133 bp
  • T to A, chromosome 17 at 51,740,346 bp
  • C to T, chromosome 17 at 53,679,663 bp
  • T to A, chromosome 17 at 57,402,841 bp
  • T to C, chromosome 17 at 71,431,236 bp
  • C to T, chromosome 17 at 72,846,337 bp
  • T to C, chromosome 18 at 77,380,560 bp
  • G to A, chromosome 19 at 13,103,278 bp
  • A to C, chromosome 19 at 41,955,168 bp
  • T to C, chromosome 19 at 44,520,408 bp
  • T to C, chromosome X at 150,654,569 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0049 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038343-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.