Strain Name:
C57BL/6J-MtgxR0050Btlr/Mmmh
Stock Number:
038344-MU
Citation ID:
RRID:MMRRC_038344-MU
Other Names:
R0050 (G1), C57BL/6J-MtgxR0050Btlr
Major Collection:

Strain Information

Slc6a12
Name: solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Synonyms: Gabt2, BGT1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14411
Homologene: 128225
Dock9
Name: dedicator of cytokinesis 9
Synonyms: B230309H04Rik, D14Wsu89e, Zizimin1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105445
Homologene: 41026
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: D330022K21Rik, 2810449C13Rik, Usp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170707
Homologene: 12988
Rheb
Name: Ras homolog enriched in brain
Synonyms: Rheb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19744
Homologene: 123916
Skp2
Name: S-phase kinase-associated protein 2
Synonyms: FBXL1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 27401
Homologene: 55942
Trps1
Name: transcriptional repressor GATA binding 1
Synonyms: D15Ertd586e, trichorhinophalangeal syndrome I (human)
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 83925
Homologene: 8556
Abca1
Name: ATP-binding cassette, sub-family A member 1
Synonyms: ABC1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11303
HGNC: HGNC:29
Homologene: 21130
Plekhg5
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269608
Homologene: 10768
Tgif1
Name: TGFB-induced factor homeobox 1
Synonyms: Tgif
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21815
Homologene: 7574
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Ppp3cb
Name: protein phosphatase 3, catalytic subunit, beta isoform
Synonyms: PP2BA beta, Calnb, CnAbeta, 1110063J16Rik, Cnab
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19056
HGNC: HGNC:9315
Homologene: 56429
Spin1
Name: spindlin 1
Synonyms: Spin
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20729
VEGA: 13
Homologene: 55983
Usp54
Name: ubiquitin specific peptidase 54
Synonyms: 4930429G18Rik, C030002J06Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 78787
Homologene: 90902
Tgfb3
Name: transforming growth factor, beta 3
Synonyms: Tgfb-3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21809
VEGA: 12
Homologene: 2433
Scn4a
Name: sodium channel, voltage-gated, type IV, alpha
Synonyms: Nav1.4, mH2, SkM1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 110880
Homologene: 283
Trmt2a
Name: TRM2 tRNA methyltransferase 2A
Synonyms: Htf9c
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 15547
Homologene: 7374
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 192190
Homologene: 16332
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Lmntd1
Name: lamin tail domain containing 1
Synonyms: Lmna-rs1, 4933403M22Rik, Ifltd1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74071
Homologene: 69453
Asns
Name: asparagine synthetase
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 27053
HGNC: HGNC:753
Homologene: 69113
Pear1
Name: platelet endothelial aggregation receptor 1
Synonyms: 3110045G13Rik, Jedi-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73182
Homologene: 12492
Gprc6a
Name: G protein-coupled receptor, family C, group 6, member A
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 210198
VEGA: 10
Homologene: 17529
Sycp2
Name: synaptonemal complex protein 2
Synonyms: 3830402K23Rik, 4930518F03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320558
Homologene: 8604
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19886
Homologene: 2207
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Ankar
Name: ankyrin and armadillo repeat containing
Synonyms: 4932422E22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319695
Homologene: 85163
Daw1
Name: dynein assembly factor with WDR repeat domains 1
Synonyms: 4933429D11Rik, 4930563E19Rik, Wdr69, b2b1116Clo, b2b1584Clo
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71227
Atg4b
Name: autophagy related 4B, cysteine peptidase
Synonyms: Apg4b, 2510009N07Rik, autophagin 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66615
Homologene: 100868
Arhgef38
Name: Rho guanine nucleotide exchange factor 38
Synonyms: 9130221D24Rik, D630013G24Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77669
Homologene: 137396
Acnat2
Name: acyl-coenzyme A amino acid N-acyltransferase 2
Synonyms: C730036D15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 209186
Homologene: 28287
Sema3d
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Synonyms: 4631426B19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108151
Homologene: 23664
Stx2
Name: syntaxin 2
Synonyms: Epim, G1-536-1, repro34, Syn-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13852
HGNC: HGNC:3403
Homologene: 37559
Edem1
Name: ER degradation enhancer, mannosidase alpha-like 1
Synonyms: A130059K23Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 192193
Homologene: 33836
H1f8
Name: H1.8 linker histone
Synonyms: H1oo, H1foo, H1-8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171506
Homologene: 51377
Ucp1
Name: uncoupling protein 1 (mitochondrial, proton carrier)
Synonyms: Slc25a7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22227
Homologene: 22524
Ces2c
Name: carboxylesterase 2C
Synonyms: Ces2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234671
HGNC: HGNC:1864
Homologene: 138298
Mmp12
Name: matrix metallopeptidase 12
Synonyms: macrophage elastase, MMP12, Mmel
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17381
HGNC: HGNC:7158
Homologene: 20547
Mre11a
Name: MRE11A homolog A, double strand break repair nuclease
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17535
HGNC: HGNC:7230
Homologene: 4083
Oaz2
Name: ornithine decarboxylase antizyme 2
Synonyms: AZ-2, antizyme 2, Sez15, AZ2, Oaz2-ps
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18247
HGNC: HGNC:8096
Homologene: 31094
Abhd14a
Name: abhydrolase domain containing 14A
Synonyms: 1110013B16Rik, Dorz1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68644
Homologene: 22889
Parp3
Name: poly (ADP-ribose) polymerase family, member 3
Synonyms: Adprt3, PARP-3, A930002C11Rik, Adprtl3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235587
HGNC: HGNC:273
Homologene: 4005
Mtrf1l
Name: mitochondrial translational release factor 1-like
Synonyms: 9130004K12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108853
Homologene: 5905
Lrriq1
Name: leucine-rich repeats and IQ motif containing 1
Synonyms: LOC380658, 4930503E15Rik, Gm1557
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74978
Homologene: 46007
Adamts2
Name: ADAM metallopeptidase with thrombospondin type 1 motif 2
Synonyms: ADAM-TS2, hPCPNI, a disintegrin and metalloproteinase with thrombospondin repeats, procollagen N-proteinase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216725
HGNC: HGNC:218
Homologene: 8597
Cpd
Name: carboxypeptidase D
Synonyms: D830034L15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12874
HGNC: HGNC:2301
Homologene: 999
Gm11492
Name: predicted gene 11492
Type: Gene
Species: Mouse
Chromosome: 11
Abca9
Name: ATP-binding cassette, sub-family A member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Slc8a3
Name: solute carrier family 8 (sodium/calcium exchanger), member 3
Synonyms: Ncx3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 110893
Homologene: 62645
Akr1c13
Name: aldo-keto reductase family 1, member C13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27384
HGNC: HGNC:387
Homologene: 121208
Adcy5
Name: adenylate cyclase 5
Synonyms: AC5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224129
VEGA: 16
HGNC: HGNC:236
Homologene: 11213
Cadm2
Name: cell adhesion molecule 2
Synonyms: A830029E02Rik, Necl3, SynCAM2, Igsf4d, 2900078E11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239857
Homologene: 17705
Dmrt3
Name: doublesex and mab-3 related transcription factor 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240590
VEGA: 19
Homologene: 10935
Ermp1
Name: endoplasmic reticulum metallopeptidase 1
Synonyms: D19Ertd410e, D19Wsu12e, b2b2633Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226090
Homologene: 121891
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 72,656,164 bp
  • T to C, chromosome 1 at 83,180,365 bp
  • T to A, chromosome 1 at 93,787,718 bp
  • T to C, chromosome 1 at 158,579,724 bp
  • T to A, chromosome 2 at 32,292,127 bp
  • A to T, chromosome 2 at 178,364,711 bp
  • G to T, chromosome 3 at 87,755,987 bp
  • C to A, chromosome 3 at 133,132,196 bp
  • G to A, chromosome 4 at 49,380,586 bp
  • A to G, chromosome 4 at 53,069,910 bp
  • C to A, chromosome 4 at 137,613,803 bp
  • T to C, chromosome 4 at 152,108,088 bp
  • T to A, chromosome 5 at 12,584,953 bp
  • A to T, chromosome 5 at 24,817,834 bp
  • A to G, chromosome 5 at 124,830,744 bp
  • A to G, chromosome 5 at 128,999,508 bp
  • T to C, chromosome 6 at 7,676,019 bp
  • T to C, chromosome 6 at 108,828,848 bp
  • G to T, chromosome 6 at 115,947,768 bp
  • T to C, chromosome 6 at 121,360,419 bp
  • T to C, chromosome 6 at 145,417,476 bp
  • A to G, chromosome 8 at 83,294,228 bp
  • T to A, chromosome 8 at 104,848,199 bp
  • A to G, chromosome 9 at 7,350,152 bp
  • A to G, chromosome 9 at 14,830,973 bp
  • A to G, chromosome 9 at 65,687,802 bp
  • A to T, chromosome 9 at 106,440,883 bp
  • A to T, chromosome 9 at 106,471,401 bp
  • T to C, chromosome 10 at 5,815,553 bp
  • T to A, chromosome 10 at 51,615,389 bp
  • G to A, chromosome 10 at 52,101,803 bp
  • A to G, chromosome 10 at 103,068,931 bp
  • T to C, chromosome 11 at 50,775,395 bp
  • T to A, chromosome 11 at 76,792,859 bp
  • T to C, chromosome 11 at 87,567,346 bp
  • C to G, chromosome 11 at 106,320,856 bp
  • C to T, chromosome 11 at 110,145,591 bp
  • T to C, chromosome 12 at 81,315,265 bp
  • T to A, chromosome 12 at 86,069,884 bp
  • T to C, chromosome 13 at 4,194,670 bp
  • T to A, chromosome 13 at 51,150,418 bp
  • A to G, chromosome 14 at 20,531,752 bp
  • A to T, chromosome 14 at 20,573,755 bp
  • A to G, chromosome 14 at 121,607,225 bp
  • A to G, chromosome 15 at 9,125,091 bp
  • A to T, chromosome 15 at 44,573,807 bp
  • A to T, chromosome 15 at 50,765,525 bp
  • A to T, chromosome 16 at 18,250,843 bp
  • A to G, chromosome 16 at 35,304,303 bp
  • A to G, chromosome 16 at 66,953,266 bp
  • T to A, chromosome 17 at 34,673,325 bp
  • A to T, chromosome 17 at 67,782,056 bp
  • T to G, chromosome 17 at 70,850,884 bp
  • T to A, chromosome 18 at 12,404,103 bp
  • T to A, chromosome 18 at 44,156,453 bp
  • C to A, chromosome 19 at 25,622,589 bp
  • C to A, chromosome 19 at 29,628,784 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0050 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038344-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.