Strain Name:
C57BL/6J-MtgxR0218Btlr/Mmmh
Stock Number:
038467-MU
Citation ID:
RRID:MMRRC_038467-MU
Other Names:
R0218 (G1), C57BL/6J-MtgxR0218Btlr
Major Collection:

Gene Information

Gene Symbol: Dlc1 [MGI:1354949] (Mus musculus (mouse))
Name: deleted in liver cancer 1
Synonyms: Arhgap7, p122-RhoGAP, STARD12, A730069N07Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Ciapin1 [MGI:1922083] (Mus musculus (mouse))
Name: cytokine induced apoptosis inhibitor 1
Synonyms: anamorsin, 2810413N20Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Sephs1 [MGI:1923580] (Mus musculus (mouse))
Name: selenophosphate synthetase 1
Synonyms: SPS1, 1110046B24Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Gpr1 [MGI:2385324] (Mus musculus (mouse))
Name: G protein-coupled receptor 1
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Washc2 [MGI:106463] (Mus musculus (mouse))
Name: WASH complex subunit 2`
Synonyms: D6Wsu116e, Fam21, C530005J20Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Cenpp [MGI:1913586] (Mus musculus (mouse))
Name: centromere protein P
Synonyms: 4921518G09Rik, 1700022C02Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Fancl [MGI:1914280] (Mus musculus (mouse))
Name: Fanconi anemia, complementation group L
Synonyms: 2010322C19Rik, gcd, B230118H11Rik, Pog, Phf9
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 63,183,531 bp
  • C to T, chromosome 1 at 166,238,590 bp
  • A to G, chromosome 2 at 4,899,560 bp
  • G to T, chromosome 2 at 35,028,074 bp
  • T to A, chromosome 2 at 119,353,460 bp
  • A to G, chromosome 2 at 121,305,425 bp
  • A to G, chromosome 4 at 143,851,831 bp
  • C to A, chromosome 6 at 57,619,601 bp
  • T to C, chromosome 6 at 115,814,650 bp
  • T to A, chromosome 6 at 116,248,046 bp
  • T to G, chromosome 6 at 131,685,092 bp
  • C to T, chromosome 7 at 16,461,893 bp
  • A to G, chromosome 7 at 29,793,638 bp
  • T to C, chromosome 7 at 47,309,406 bp
  • A to G, chromosome 7 at 73,740,764 bp
  • A to T, chromosome 7 at 82,869,104 bp
  • T to A, chromosome 7 at 108,755,574 bp
  • T to C, chromosome 7 at 121,998,900 bp
  • A to G, chromosome 8 at 36,850,229 bp
  • T to G, chromosome 8 at 43,626,440 bp
  • A to G, chromosome 8 at 45,934,724 bp
  • G to T, chromosome 8 at 94,828,310 bp
  • A to G, chromosome 9 at 87,211,809 bp
  • T to C, chromosome 10 at 17,915,715 bp
  • A to T, chromosome 10 at 24,776,869 bp
  • A to G, chromosome 10 at 118,156,878 bp
  • A to T, chromosome 11 at 22,231,992 bp
  • A to G, chromosome 11 at 26,471,337 bp
  • A to G, chromosome 11 at 82,911,860 bp
  • A to G, chromosome 11 at 87,086,122 bp
  • A to T, chromosome 11 at 96,827,613 bp
  • A to G, chromosome 11 at 102,406,230 bp
  • A to G, chromosome 12 at 9,579,639 bp
  • A to G, chromosome 12 at 66,655,120 bp
  • A to T, chromosome 12 at 69,790,035 bp
  • A to T, chromosome 12 at 86,882,624 bp
  • A to G, chromosome 12 at 98,222,647 bp
  • A to G, chromosome 12 at 103,108,781 bp
  • T to A, chromosome 13 at 49,647,632 bp
  • T to C, chromosome 13 at 54,526,604 bp
  • A to T, chromosome 13 at 62,854,048 bp
  • C to T, chromosome 13 at 81,106,898 bp
  • A to T, chromosome 14 at 20,523,976 bp
  • A to G, chromosome 16 at 17,849,786 bp
  • T to A, chromosome 16 at 44,788,743 bp
  • C to T, chromosome 16 at 59,036,093 bp
  • T to C, chromosome 18 at 36,676,609 bp
  • C to G, chromosome 18 at 44,519,516 bp
  • A to G, chromosome 18 at 52,907,328 bp
  • A to G, chromosome 18 at 61,020,364 bp
  • A to T, chromosome 19 at 11,116,437 bp
  • T to C, chromosome 19 at 13,814,978 bp
  • A to G, chromosome 19 at 40,912,628 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R0218 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    038467-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.