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Strain Name:
C57BL/6J-MtgxR0239Btlr/Mmmh
Stock Number:
038477-MU
Citation ID:
RRID:MMRRC_038477-MU
Other Names:
R0239 (G1), C57BL/6J-MtgxR0239Btlr
Major Collection:

Strain Information

Ptk2
Name: PTK2 protein tyrosine kinase 2
Synonyms: Fadk, FAK, FRNK
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14083
VEGA: 15
HGNC: HGNC:9611
Homologene: 7314
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Slc6a1
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Synonyms: XT-1, Gabt, Xtrp1, Gat1, Gabt1, GAT-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232333
Homologene: 2290
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,828,050 bp
  • A to G, chromosome 1 at 66,416,106 bp
  • T to C, chromosome 1 at 91,322,287 bp
  • T to C, chromosome 1 at 134,329,189 bp
  • A to G, chromosome 1 at 135,404,336 bp
  • A to G, chromosome 1 at 136,527,393 bp
  • A to T, chromosome 1 at 193,321,053 bp
  • T to G, chromosome 2 at 26,062,261 bp
  • T to C, chromosome 2 at 36,177,281 bp
  • T to A, chromosome 2 at 39,075,042 bp
  • T to A, chromosome 2 at 70,105,425 bp
  • T to C, chromosome 2 at 87,458,037 bp
  • C to A, chromosome 2 at 131,546,214 bp
  • T to C, chromosome 2 at 152,008,156 bp
  • C to T, chromosome 2 at 165,280,837 bp
  • A to G, chromosome 3 at 15,296,601 bp
  • A to G, chromosome 3 at 41,753,878 bp
  • A to G, chromosome 3 at 88,333,321 bp
  • A to G, chromosome 3 at 89,067,222 bp
  • A to G, chromosome 3 at 93,774,032 bp
  • TTCCTCCTCCTCCTCCTCCTCC to TTCCTCCTCCTCCTCCTCC, chromosome 3 at 138,065,834 bp
  • A to G, chromosome 4 at 43,453,163 bp
  • C to A, chromosome 4 at 56,784,596 bp
  • T to C, chromosome 4 at 108,595,646 bp
  • A to G, chromosome 4 at 151,143,730 bp
  • T to C, chromosome 5 at 32,943,240 bp
  • A to T, chromosome 5 at 63,800,124 bp
  • G to T, chromosome 5 at 108,444,016 bp
  • C to T, chromosome 5 at 129,658,238 bp
  • G to T, chromosome 5 at 147,303,287 bp
  • T to A, chromosome 6 at 24,876,344 bp
  • C to T, chromosome 6 at 84,064,479 bp
  • G to A, chromosome 6 at 114,302,800 bp
  • G to T, chromosome 6 at 115,968,793 bp
  • C to T, chromosome 6 at 128,489,156 bp
  • G to A, chromosome 6 at 145,171,978 bp
  • T to A, chromosome 7 at 31,291,730 bp
  • A to G, chromosome 7 at 45,532,358 bp
  • T to C, chromosome 7 at 46,079,594 bp
  • A to T, chromosome 7 at 97,383,684 bp
  • G to A, chromosome 7 at 103,212,726 bp
  • A to G, chromosome 7 at 105,721,531 bp
  • A to G, chromosome 7 at 106,689,255 bp
  • T to C, chromosome 7 at 125,575,199 bp
  • G to T, chromosome 7 at 143,082,958 bp
  • C to T, chromosome 8 at 11,218,780 bp
  • T to A, chromosome 8 at 105,844,488 bp
  • A to T, chromosome 8 at 110,635,375 bp
  • G to T, chromosome 9 at 4,330,144 bp
  • C to T, chromosome 9 at 22,249,759 bp
  • T to C, chromosome 9 at 55,163,007 bp
  • C to A, chromosome 10 at 22,180,862 bp
  • C to T, chromosome 10 at 87,567,281 bp
  • T to C, chromosome 10 at 93,503,482 bp
  • C to T, chromosome 10 at 105,826,525 bp
  • T to A, chromosome 10 at 107,806,696 bp
  • A to G, chromosome 11 at 46,150,441 bp
  • T to C, chromosome 11 at 59,801,076 bp
  • A to G, chromosome 11 at 67,301,692 bp
  • A to T, chromosome 11 at 79,418,574 bp
  • G to A, chromosome 11 at 100,260,878 bp
  • C to A, chromosome 12 at 17,294,675 bp
  • G to T, chromosome 12 at 40,737,540 bp
  • T to A, chromosome 12 at 51,769,318 bp
  • T to C, chromosome 12 at 87,075,639 bp
  • C to T, chromosome 12 at 101,884,728 bp
  • A to G, chromosome 12 at 111,785,324 bp
  • A to C, chromosome 14 at 20,448,605 bp
  • A to G, chromosome 14 at 30,123,496 bp
  • A to T, chromosome 14 at 49,304,256 bp
  • G to A, chromosome 14 at 57,917,378 bp
  • A to G, chromosome 14 at 69,720,997 bp
  • T to C, chromosome 15 at 21,586,407 bp
  • C to A, chromosome 15 at 57,940,753 bp
  • A to T, chromosome 15 at 73,343,283 bp
  • T to C, chromosome 17 at 24,884,471 bp
  • C to A, chromosome 17 at 25,045,523 bp
  • C to A, chromosome 17 at 80,225,710 bp
  • C to T, chromosome 18 at 10,458,567 bp
  • A to T, chromosome 18 at 65,682,985 bp
  • G to T, chromosome 19 at 6,071,437 bp
  • A to G, chromosome 19 at 10,669,453 bp
  • G to A, chromosome 19 at 44,525,469 bp
  • T to A, chromosome 19 at 57,682,359 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0239 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038477-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.