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Strain Name:
C57BL/6J-MtgxR0240Btlr/Mmmh
Stock Number:
038478-MU
Citation ID:
RRID:MMRRC_038478-MU
Other Names:
R0240 (G1), C57BL/6J-MtgxR0240Btlr
Major Collection:

Strain Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Gabra6
Name: gamma-aminobutyric acid type A receptor subunit alpha 6
Synonyms: Gabra-6, alpha6, GABA-ARalpha6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14399
HGNC: HGNC:4080
Homologene: 20220
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Nr2f2
Name: nuclear receptor subfamily 2, group F, member 2
Synonyms: COUP-TFII, ARP-1, Tcfcoup2, Aporp1, COUP-TF2, 9430015G03Rik, EAR3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11819
HGNC: HGNC:7976
Homologene: 7628
Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 93,627,060 bp
  • A to G, chromosome 1 at 132,601,983 bp
  • T to C, chromosome 1 at 136,073,496 bp
  • G to A, chromosome 1 at 151,457,454 bp
  • T to C, chromosome 1 at 152,554,424 bp
  • G to T, chromosome 1 at 162,353,625 bp
  • G to A, chromosome 1 at 168,203,482 bp
  • T to A, chromosome 1 at 192,505,340 bp
  • T to C, chromosome 1 at 193,335,027 bp
  • G to A, chromosome 2 at 24,638,657 bp
  • T to C, chromosome 2 at 25,236,207 bp
  • A to G, chromosome 2 at 32,955,185 bp
  • G to T, chromosome 2 at 65,735,774 bp
  • A to T, chromosome 2 at 84,675,842 bp
  • A to G, chromosome 2 at 86,483,386 bp
  • A to T, chromosome 2 at 88,973,396 bp
  • C to A, chromosome 2 at 118,114,393 bp
  • T to A, chromosome 2 at 122,454,527 bp
  • A to T, chromosome 2 at 126,920,336 bp
  • A to G, chromosome 2 at 166,054,400 bp
  • A to G, chromosome 3 at 108,858,919 bp
  • T to C, chromosome 4 at 8,852,670 bp
  • C to A, chromosome 4 at 14,532,651 bp
  • C to T, chromosome 4 at 19,641,734 bp
  • T to A, chromosome 4 at 43,854,512 bp
  • A to T, chromosome 4 at 57,232,374 bp
  • C to A, chromosome 4 at 106,414,404 bp
  • T to A, chromosome 4 at 108,098,078 bp
  • T to G, chromosome 4 at 117,002,613 bp
  • C to A, chromosome 4 at 132,332,915 bp
  • C to A, chromosome 4 at 133,848,531 bp
  • T to A, chromosome 4 at 137,949,435 bp
  • A to G, chromosome 4 at 138,170,562 bp
  • T to C, chromosome 5 at 22,106,045 bp
  • C to T, chromosome 5 at 117,867,883 bp
  • G to A, chromosome 5 at 137,398,362 bp
  • T to C, chromosome 5 at 141,998,747 bp
  • A to G, chromosome 6 at 39,337,238 bp
  • T to A, chromosome 6 at 39,583,818 bp
  • G to C, chromosome 6 at 90,223,943 bp
  • A to G, chromosome 7 at 10,159,400 bp
  • C to A, chromosome 7 at 19,184,872 bp
  • T to C, chromosome 7 at 26,462,516 bp
  • T to A, chromosome 7 at 35,619,439 bp
  • C to T, chromosome 7 at 45,200,251 bp
  • C to A, chromosome 7 at 46,264,032 bp
  • G to C, chromosome 7 at 70,360,175 bp
  • T to C, chromosome 7 at 143,741,669 bp
  • T to A, chromosome 8 at 22,065,452 bp
  • A to T, chromosome 8 at 25,739,590 bp
  • A to G, chromosome 8 at 56,590,261 bp
  • T to C, chromosome 8 at 105,259,292 bp
  • T to C, chromosome 8 at 106,265,156 bp
  • C to T, chromosome 8 at 119,840,324 bp
  • T to C, chromosome 8 at 122,780,181 bp
  • A to G, chromosome 9 at 7,506,543 bp
  • T to C, chromosome 9 at 21,737,999 bp
  • G to T, chromosome 9 at 24,658,580 bp
  • C to A, chromosome 9 at 52,144,083 bp
  • A to T, chromosome 9 at 79,652,033 bp
  • A to G, chromosome 9 at 108,534,134 bp
  • T to C, chromosome 9 at 111,635,021 bp
  • A to T, chromosome 10 at 34,098,932 bp
  • T to A, chromosome 10 at 88,555,738 bp
  • A to T, chromosome 11 at 23,433,206 bp
  • A to G, chromosome 11 at 29,792,367 bp
  • C to T, chromosome 11 at 42,314,947 bp
  • G to T, chromosome 11 at 44,458,277 bp
  • A to T, chromosome 11 at 50,775,374 bp
  • G to A, chromosome 11 at 55,492,028 bp
  • A to G, chromosome 11 at 69,879,814 bp
  • A to G, chromosome 11 at 75,328,687 bp
  • A to G, chromosome 11 at 75,510,921 bp
  • A to G, chromosome 11 at 78,076,058 bp
  • G to A, chromosome 11 at 106,754,896 bp
  • A to T, chromosome 12 at 20,900,561 bp
  • T to C, chromosome 12 at 81,947,018 bp
  • A to T, chromosome 12 at 98,252,034 bp
  • G to A, chromosome 14 at 23,494,579 bp
  • T to A, chromosome 14 at 30,096,969 bp
  • T to A, chromosome 14 at 30,484,594 bp
  • G to A, chromosome 14 at 30,602,088 bp
  • A to G, chromosome 14 at 49,768,402 bp
  • C to T, chromosome 14 at 50,863,029 bp
  • T to A, chromosome 14 at 68,121,134 bp
  • T to C, chromosome 15 at 47,629,239 bp
  • T to A, chromosome 15 at 73,581,906 bp
  • C to T, chromosome 15 at 75,714,122 bp
  • T to A, chromosome 15 at 76,723,209 bp
  • T to C, chromosome 15 at 89,125,882 bp
  • T to C, chromosome 15 at 102,312,541 bp
  • C to T, chromosome 16 at 11,660,553 bp
  • A to T, chromosome 16 at 13,879,445 bp
  • A to G, chromosome 17 at 18,304,799 bp
  • T to A, chromosome 17 at 27,895,870 bp
  • T to C, chromosome 17 at 30,765,679 bp
  • C to T, chromosome 17 at 43,038,213 bp
  • C to T, chromosome 17 at 46,396,813 bp
  • T to C, chromosome 17 at 56,436,087 bp
  • C to T, chromosome 18 at 12,155,227 bp
  • A to T, chromosome 18 at 12,539,823 bp
  • A to T, chromosome 19 at 5,156,374 bp
  • A to T, chromosome 19 at 6,962,995 bp
  • A to T, chromosome 19 at 7,097,110 bp
  • A to G, chromosome 19 at 8,912,813 bp
  • A to T, chromosome 19 at 12,394,963 bp
  • G to A, chromosome 19 at 34,046,810 bp
  • A to C, chromosome 19 at 38,728,886 bp
  • T to G, chromosome X at 8,128,045 bp
  • T to A, chromosome X at 106,109,841 bp
  • C to A, chromosome X at 157,186,218 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0240 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038478-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.