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Strain Name:
C57BL/6J-MtgxR0398Btlr/Mmmh
Stock Number:
038603-MU
Citation ID:
RRID:MMRRC_038603-MU
Other Names:
R0398 (G1), C57BL/6J-MtgxR0398Btlr
Major Collection:

Strain Information

Sp4
Name: trans-acting transcription factor 4
Synonyms: HF1-b, HF-1b, 5730497N03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20688
VEGA: 12
Homologene: 2341
Cdk17
Name: cyclin dependent kinase 17
Synonyms: 6430598J10Rik, Pctk2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237459
VEGA: 10
HGNC: HGNC:8750
Homologene: 55666
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
E2f2
Name: E2F transcription factor 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242705
HGNC: HGNC:3114
Homologene: 48264
Jak2
Name: Janus kinase 2
Synonyms: C81284
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16452
VEGA: 19
HGNC: HGNC:6192
Homologene: 21033
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Gart
Name: phosphoribosylglycinamide formyltransferase
Synonyms: Gaps, Prgs
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 14450
HGNC: HGNC:4163
Homologene: 637
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 33,484,676 bp
  • A to G, chromosome 1 at 36,632,406 bp
  • AT to A, chromosome 1 at 88,212,371 bp
  • C to A, chromosome 1 at 150,798,814 bp
  • C to A, chromosome 1 at 172,317,499 bp
  • T to C, chromosome 1 at 185,338,158 bp
  • T to A, chromosome 1 at 191,827,361 bp
  • C to A, chromosome 2 at 33,411,048 bp
  • T to C, chromosome 2 at 69,286,666 bp
  • A to G, chromosome 2 at 72,031,041 bp
  • A to T, chromosome 2 at 87,858,057 bp
  • T to G, chromosome 2 at 120,696,307 bp
  • C to G, chromosome 2 at 180,593,699 bp
  • A to G, chromosome 3 at 27,461,779 bp
  • C to T, chromosome 3 at 36,181,052 bp
  • A to G, chromosome 3 at 36,590,717 bp
  • G to A, chromosome 3 at 40,756,997 bp
  • C to A, chromosome 3 at 129,890,396 bp
  • T to C, chromosome 3 at 142,545,513 bp
  • A to G, chromosome 4 at 63,357,648 bp
  • T to C, chromosome 4 at 106,667,573 bp
  • T to A, chromosome 4 at 123,351,017 bp
  • T to A, chromosome 4 at 136,180,544 bp
  • C to A, chromosome 4 at 149,153,882 bp
  • T to C, chromosome 4 at 149,204,231 bp
  • A to G, chromosome 5 at 9,445,367 bp
  • A to G, chromosome 5 at 14,681,702 bp
  • T to C, chromosome 5 at 141,962,721 bp
  • T to A, chromosome 6 at 3,374,502 bp
  • A to T, chromosome 6 at 24,756,671 bp
  • A to G, chromosome 6 at 30,645,251 bp
  • C to T, chromosome 7 at 28,287,901 bp
  • C to T, chromosome 7 at 62,380,551 bp
  • T to A, chromosome 7 at 102,542,692 bp
  • T to A, chromosome 7 at 108,612,955 bp
  • T to C, chromosome 7 at 126,450,418 bp
  • A to G, chromosome 8 at 24,813,432 bp
  • G to A, chromosome 8 at 70,499,089 bp
  • A to G, chromosome 8 at 108,951,246 bp
  • T to G, chromosome 8 at 119,314,047 bp
  • T to C, chromosome 9 at 15,354,736 bp
  • T to C, chromosome 9 at 53,884,169 bp
  • A to G, chromosome 9 at 105,064,103 bp
  • T to A, chromosome 9 at 119,954,562 bp
  • T to C, chromosome 10 at 44,439,809 bp
  • T to C, chromosome 10 at 76,182,722 bp
  • T to C, chromosome 10 at 76,710,118 bp
  • C to T, chromosome 10 at 78,579,539 bp
  • A to T, chromosome 10 at 81,033,787 bp
  • T to A, chromosome 10 at 93,237,840 bp
  • G to T, chromosome 11 at 4,921,978 bp
  • T to C, chromosome 11 at 22,095,886 bp
  • C to T, chromosome 11 at 53,272,879 bp
  • C to A, chromosome 11 at 78,205,268 bp
  • A to C, chromosome 11 at 96,283,111 bp
  • A to T, chromosome 11 at 96,942,427 bp
  • A to G, chromosome 11 at 101,325,553 bp
  • A to G, chromosome 11 at 106,261,544 bp
  • A to G, chromosome 11 at 115,617,575 bp
  • A to T, chromosome 12 at 31,492,092 bp
  • A to T, chromosome 12 at 118,298,673 bp
  • A to T, chromosome 13 at 65,194,918 bp
  • A to C, chromosome 14 at 67,697,962 bp
  • T to C, chromosome 14 at 75,126,470 bp
  • G to A, chromosome 15 at 53,719,720 bp
  • G to C, chromosome 15 at 76,905,046 bp
  • T to G, chromosome 15 at 89,573,941 bp
  • T to C, chromosome 15 at 99,628,627 bp
  • T to A, chromosome 16 at 35,269,068 bp
  • G to T, chromosome 16 at 91,639,449 bp
  • A to G, chromosome 16 at 95,716,223 bp
  • G to A, chromosome 17 at 29,145,370 bp
  • G to T, chromosome 17 at 31,617,242 bp
  • A to G, chromosome 17 at 42,377,259 bp
  • A to G, chromosome 17 at 46,251,485 bp
  • A to C, chromosome 17 at 74,445,920 bp
  • A to G, chromosome 18 at 46,880,693 bp
  • C to T, chromosome 19 at 4,263,699 bp
  • T to G, chromosome 19 at 4,601,610 bp
  • T to G, chromosome 19 at 6,246,578 bp
  • T to A, chromosome 19 at 29,282,388 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0398 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038603-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.