Strain Name:
C57BL/6J-MtgxR0448Btlr/Mmmh
Stock Number:
038648-MU
Citation ID:
RRID:MMRRC_038648-MU
Other Names:
R0448 (G1), C57BL/6J-MtgxR0448Btlr
Major Collection:

Gene Information

Msx2
Name: msh homeobox 2
Synonyms: Hox8.1, Hox8, Hox-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17702
HGNC: HGNC:7392
Homologene: 1837
Arhgef7
Name: Rho guanine nucleotide exchange factor (GEF7)
Synonyms: Cool, PIX, Pak interacting exchange factor, p85SPR, betaPix-c, betaPix-b, cool-1, betaPix
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 54126
Homologene: 2895
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna-2, 2610027H02Rik, Spna2, alphaII-spectrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20740
Homologene: 2353
Zmym6
Name: zinc finger, MYM-type 6
Synonyms: D4Wsu24e, 9330177P20Rik, Zfp258
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100177
Homologene: 5186
Sephs1
Name: selenophosphate synthetase 1
Synonyms: 1110046B24Rik, SPS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 109079
Homologene: 56558
Zfp101
Name: zinc finger protein 101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22643
Homologene: 137226
Csnk1g1
Name: casein kinase 1, gamma 1
Synonyms: 9130020E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214897
HGNC: HGNC:2454
Homologene: 48692
Brpf3
Name: bromodomain and PHD finger containing, 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268936
Homologene: 16092
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100683
Homologene: 39246
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21819
Homologene: 2430
Lrpprc
Name: leucine-rich PPR-motif containing
Synonyms: Lrp130, 3110001K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72416
Homologene: 32695
Abcc5
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
Synonyms: Mrp5, Abcc5b, Abcc5a, 2900011L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 27416
HGNC: HGNC:56
Homologene: 21164
Ahi1
Name: Abelson helper integration site 1
Synonyms: Ahi-1, 1700015F03Rik, D10Bwg0629e, Jouberin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52906
VEGA: 10
Homologene: 9762
Pggt1b
Name: protein geranylgeranyltransferase type I, beta subunit
Synonyms: GGT1, BGG1, 2010207C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225467
VEGA: 18
HGNC: HGNC:8895
Homologene: 3685
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: cI-46, LOC381026, 9330188B09Rik, D630001M17Rik, 4921513O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66725
Homologene: 18982
Crebrf
Name: CREB3 regulatory factor
Synonyms: A930001N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77128
Homologene: 12672
Epg5
Name: ectopic P-granules autophagy protein 5 homolog (C. elegans)
Synonyms: 5430411K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Sorl1
Name: sortilin-related receptor, LDLR class A repeats-containing
Synonyms: LR11, mSorLA, 2900010L19Rik, Sorla
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20660
Homologene: 2336
Copg1
Name: coatomer protein complex, subunit gamma 1
Synonyms: D6Ertd71e, D6Wsu16e, Copg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54161
HGNC: HGNC:2236
Homologene: 56745
Nup153
Name: nucleoporin 153
Synonyms: B130015D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218210
HGNC: HGNC:8062
Homologene: 68442
Thoc6
Name: THO complex 6
Synonyms: F830014G06Rik, Wdr58
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 386612
Homologene: 11533
Wdtc1
Name: WD and tetratricopeptide repeats 1
Synonyms: LOC230796, adp, adipose
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230796
Homologene: 8997
H2-T22
Name: histocompatibility 2, T region locus 22
Synonyms: H-2T22, H-2T17, H2-T17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15039
Homologene: 79525
Ercc5
Name: excision repair cross-complementing rodent repair deficiency, complementation group 5
Synonyms: Xpg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22592
HGNC: HGNC:3437
Homologene: 133551
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dchs1
Name: dachsous cadherin related 1
Synonyms: C130033F22Rik, 3110041P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233651
Homologene: 2771
Txndc11
Name: thioredoxin domain containing 11
Synonyms: EF-hand binding protein 1, EFP1, Txdc11, 2810408E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106200
Homologene: 9301
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 545260
Homologene: 44428
Hephl1
Name: hephaestin-like 1
Synonyms: LOC244698, Zp, zyklopen, thd, cw
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244698
Homologene: 9112
Add2
Name: adducin 2 (beta)
Synonyms: 2900072M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11519
HGNC: HGNC:244
Homologene: 1221
Flt1
Name: FMS-like tyrosine kinase 1
Synonyms: vascular endothelial growth factor receptor-1, VEGFR-1, VEGFR1, Flt-1, sFlt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14254
HGNC: HGNC:3763
Homologene: 134179
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Cryga
Name: crystallin, gamma A
Synonyms: DGcry-4, Cryg-4, Secc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12964
HGNC: HGNC:2408
Homologene: 129704
Pik3r2
Name: phosphoinositide-3-kinase regulatory subunit 2
Synonyms: p85beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18709
HGNC: HGNC:8980
Homologene: 3687
9530053A07Rik
Name: RIKEN cDNA 9530053A07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 319482
Homologene: 130055
Tril
Name: TLR4 interactor with leucine-rich repeats
Synonyms: 1200009O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66873
Homologene: 69404
Serpina12
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Synonyms: vaspin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68054
Homologene: 23588
Pard3b
Name: par-3 family cell polarity regulator beta
Synonyms: 2810455B10Rik, PAR3beta, PAR3L, PAR3B, 2010002N16Rik, Als2cr19, 1810008K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72823
Homologene: 35389
Rufy2
Name: RUN and FYVE domain-containing 2
Synonyms: LZ-FYVE, 2610111M19Rik, Denn, ZFYVE13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70432
Homologene: 23064
Serpinb1b
Name: serine (or cysteine) peptidase inhibitor, clade B, member 1b
Synonyms: 6330533H24Rik, ovalbumin, EIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 282663
HGNC: HGNC:3311
Homologene: 138408
Syne4
Name: spectrin repeat containing, nuclear envelope family member 4
Synonyms: 0610012K07Rik, AI428936, nesprin-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233066
Homologene: 17716
Doxl1
Name: diamine oxidase-like protein 1
Synonyms: 1600015I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69761
Homologene: 79726
Krt76
Name: keratin 76
Synonyms: 2310001L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77055
Homologene: 22931
Cyp2j6
Name: cytochrome P450, family 2, subfamily j, polypeptide 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13110
HGNC: HGNC:2634
Homologene: 68091
Adam9
Name: a disintegrin and metallopeptidase domain 9 (meltrin gamma)
Synonyms: MDC9, Mltng, MDC9, Mltng
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11502
HGNC: HGNC:216
Homologene: 20824
Crocc
Name: ciliary rootlet coiled-coil, rootletin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230872
Homologene: 16811
Sftpc
Name: surfactant associated protein C
Synonyms: SP-C, Sftp-2, Sftp2, pro-SpC, Bricd6, SPC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 20389
VEGA: 14
Homologene: 2271
Mboat1
Name: membrane bound O-acyltransferase domain containing 1
Synonyms: 9130215M02Rik, Oact1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218121
Homologene: 57467
Cdc20b
Name: cell division cycle 20B
Synonyms: EG238896, EG622422
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238896
Homologene: 65056
Hsdl2
Name: hydroxysteroid dehydrogenase like 2
Synonyms: 2610207I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72479
Tcam1
Name: testicular cell adhesion molecule 1
Synonyms: 4930570F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75870
Homologene: 123944
Grip2
Name: glutamate receptor interacting protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243547
Homologene: 16327
Kcnh8
Name: potassium voltage-gated channel, subfamily H (eag-related), member 8
Synonyms: Kv12.1, ELK1, C130090D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 211468
Homologene: 14332
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 639774
Homologene: 106613
Mcmdc2
Name: minichromosome maintenance domain containing 2
Synonyms: 6030422M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240697
Homologene: 18309
Slc25a24
Name: solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 24
Synonyms: 2610016M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229731
Homologene: 92693
Rcbtb2
Name: regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2
Synonyms: 2810420M18Rik, 2610028E02Rik, Chc1l, Rc/btb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105670
HGNC: HGNC:1914
Homologene: 970
Cyp3a11
Name: cytochrome P450, family 3, subfamily a, polypeptide 11
Synonyms: IIIAm1, Cyp3a, Pcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13112
HGNC: HGNC:2638
Homologene: 133568
Prr14
Name: proline rich 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233895
Homologene: 11424
Cnot6l
Name: CCR4-NOT transcription complex, subunit 6-like
Synonyms: 4932442K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231464
Homologene: 100830
Olfr130
Name: olfactory receptor 130
Synonyms: GA_x6K02T2PSCP-2515350-2516303, MOR256-19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258480
Homologene: 105876
S1pr5
Name: sphingosine-1-phosphate receptor 5
Synonyms: lpB4, S1P5, Edg8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 94226
Homologene: 11031
Dqx1
Name: DEAQ RNA-dependent ATPase
Synonyms: 2310066E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93838
Homologene: 14143
Vmn2r95
Name: vomeronasal 2, receptor 95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328759
Homologene: 115024
Olfr1295
Name: olfactory receptor 1295
Synonyms: GA_x6K02T2Q125-72616944-72616006, MOR248-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258398
Homologene: 74058
Vmn1r40
Name: vomeronasal 1 receptor 40
Synonyms: V1rb7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113855
Homologene: 113975
Tpi1
Name: triosephosphate isomerase 1
Synonyms: Tpi-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21991
Homologene: 128432
Pate11
Name: prostate and testis expressed 11
Synonyms: Gm9513
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 671003
Homologene: 86450
Slc25a11
Name: solute carrier family 25 (mitochondrial carrier oxoglutarate carrier), member 11
Synonyms: 2310022P18Rik, 2oxoc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67863
Homologene: 2637
Nfatc4
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4
Synonyms: 3110041H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 73181
VEGA: 14
HGNC: HGNC:7778
Homologene: 3349
Brcc3
Name: BRCA1/BRCA2-containing complex, subunit 3
Synonyms: C6.1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 210766
Homologene: 11530
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 9,940,542 bp
  • T to C, chromosome 1 at 44,173,940 bp
  • T to C, chromosome 1 at 62,166,469 bp
  • T to C, chromosome 1 at 65,103,159 bp
  • A to G, chromosome 2 at 4,899,560 bp
  • T to A, chromosome 2 at 30,026,810 bp
  • T to C, chromosome 2 at 76,720,939 bp
  • A to T, chromosome 2 at 76,761,280 bp
  • T to A, chromosome 2 at 111,565,214 bp
  • T to C, chromosome 3 at 109,157,016 bp
  • T to A, chromosome 4 at 59,606,523 bp
  • A to G, chromosome 4 at 96,545,728 bp
  • T to A, chromosome 4 at 111,936,890 bp
  • A to G, chromosome 4 at 127,108,694 bp
  • A to G, chromosome 4 at 133,297,500 bp
  • A to T, chromosome 4 at 141,042,191 bp
  • T to A, chromosome 5 at 96,080,046 bp
  • T to A, chromosome 5 at 144,839,567 bp
  • T to C, chromosome 5 at 145,862,394 bp
  • C to T, chromosome 5 at 147,566,394 bp
  • A to T, chromosome 6 at 48,933,057 bp
  • A to G, chromosome 6 at 53,817,808 bp
  • T to C, chromosome 6 at 83,060,345 bp
  • G to A, chromosome 6 at 86,092,919 bp
  • G to A, chromosome 6 at 87,904,926 bp
  • C to T, chromosome 6 at 89,714,660 bp
  • A to G, chromosome 6 at 91,779,213 bp
  • A to G, chromosome 6 at 124,814,103 bp
  • T to C, chromosome 7 at 28,140,235 bp
  • A to G, chromosome 7 at 30,314,920 bp
  • C to A, chromosome 7 at 105,765,927 bp
  • A to G, chromosome 7 at 127,474,726 bp
  • A to G, chromosome 8 at 11,819,659 bp
  • A to G, chromosome 8 at 24,964,910 bp
  • A to G, chromosome 8 at 70,772,044 bp
  • C to T, chromosome 9 at 15,076,926 bp
  • T to A, chromosome 9 at 21,244,207 bp
  • T to A, chromosome 9 at 36,477,116 bp
  • C to G, chromosome 9 at 42,004,088 bp
  • T to C, chromosome 9 at 65,980,948 bp
  • G to A, chromosome 10 at 20,972,075 bp
  • G to A, chromosome 10 at 63,004,736 bp
  • T to C, chromosome 11 at 65,918,713 bp
  • T to C, chromosome 11 at 70,645,579 bp
  • G to A, chromosome 11 at 101,508,221 bp
  • G to A, chromosome 11 at 106,284,078 bp
  • A to G, chromosome 12 at 104,038,095 bp
  • G to T, chromosome 13 at 30,202,410 bp
  • T to G, chromosome 13 at 33,089,692 bp
  • T to C, chromosome 13 at 46,717,181 bp
  • C to A, chromosome 13 at 53,468,395 bp
  • T to A, chromosome 13 at 113,078,657 bp
  • T to G, chromosome 14 at 55,831,654 bp
  • C to T, chromosome 14 at 70,522,680 bp
  • T to C, chromosome 14 at 73,178,429 bp
  • C to A, chromosome 15 at 66,764,442 bp
  • T to G, chromosome 15 at 91,709,305 bp
  • T to C, chromosome 15 at 101,890,647 bp
  • A to G, chromosome 16 at 11,091,761 bp
  • G to A, chromosome 16 at 20,399,937 bp
  • A to G, chromosome 17 at 18,451,743 bp
  • T to A, chromosome 17 at 23,669,576 bp
  • A to G, chromosome 17 at 26,743,102 bp
  • A to T, chromosome 17 at 28,806,036 bp
  • A to G, chromosome 17 at 33,382,321 bp
  • A to G, chromosome 17 at 36,042,386 bp
  • G to T, chromosome 17 at 38,067,672 bp
  • C to A, chromosome 17 at 52,977,620 bp
  • A to T, chromosome 17 at 84,770,894 bp
  • A to T, chromosome 18 at 46,262,972 bp
  • T to C, chromosome 18 at 60,917,302 bp
  • A to G, chromosome 18 at 78,023,365 bp
  • T to A, chromosome X at 75,450,041 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0448 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038648-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.