Strain Name:
C57BL/6J-MtgxR0653Btlr/Mmmh
Stock Number:
038838-MU
Citation ID:
RRID:MMRRC_038838-MU
Other Names:
R0653 (G1), C57BL/6J-MtgxR0653Btlr
Major Collection:

Gene Information

Casq2
Name: calsequestrin 2
Synonyms: ESTM52, cardiac calsequestrin, Csq2, cCSQ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12373
HGNC: HGNC:1513
Homologene: 20330
Hgs
Name: HGF-regulated tyrosine kinase substrate
Synonyms: tn, Hrs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 15239
HGNC: HGNC:4897
Homologene: 37954
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Taok1
Name: TAO kinase 1
Synonyms: D130018F14Rik, 2810468K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216965
Homologene: 27041
Fos
Name: FBJ osteosarcoma oncogene
Synonyms: D12Rfj1, cFos, c-fos
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 14281
HGNC: HGNC:3796
Homologene: 3844
Nr5a2
Name: nuclear receptor subfamily 5, group A, member 2
Synonyms: UF2-H3B, LRH-1, D1Ertd308e, Ftf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 26424
HGNC: HGNC:7984
Homologene: 20827
Zfhx3
Name: zinc finger homeobox 3
Synonyms: Sci, A230102L03Rik, WBP9, Atbf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Rtn4
Name: reticulon 4
Synonyms: Nogo-A, C130026I10Rik, NOGO, NgA, 1110020G17Rik, Nogo-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 68585
Homologene: 10743
Ranbp9
Name: RAN binding protein 9
Synonyms: RanBPM, IBAP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 56705
VEGA: 13
Homologene: 38057
Sbno1
Name: strawberry notch 1
Synonyms: 9330180L10Rik, sno
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 243272
Homologene: 10055
Atxn2
Name: ataxin 2
Synonyms: 9630045M23Rik, Sca2, ATX2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20239
Homologene: 2234
Cenpf
Name: centromere protein F
Synonyms: 6530404A22Rik, mitosin, Lek1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 108000
HGNC: HGNC:1857
Homologene: 22969
Nckap5l
Name: NCK-associated protein 5-like
Synonyms: C230021P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 380969
Homologene: 18924
Myo9a
Name: myosin IXa
Synonyms: 4732465J09Rik, C130068I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 270163
HGNC: HGNC:7608
Homologene: 21371
Zfp30
Name: zinc finger protein 30
Synonyms: 2610306P15Rik, Zfp-30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22693
Homologene: 84799
Tjp1
Name: tight junction protein 1
Synonyms: ZO-1, ZO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 21872
Homologene: 2445
Supt6
Name: SPT6, histone chaperone and transcription elongation factor
Synonyms: Supt6h, 5131400N11Rik, SPT6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20926
Homologene: 40661
Rsu1
Name: Ras suppressor protein 1
Synonyms: RsuI, rsp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20163
Homologene: 7521
Dgke
Name: diacylglycerol kinase, epsilon
Synonyms: DAGK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 56077
HGNC: HGNC:2852
Homologene: 2705
Ube2cbp
Name: ubiquitin-conjugating enzyme E2C binding protein
Synonyms: 2610018I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 70348
Homologene: 18898
Zfyve19
Name: zinc finger, FYVE domain containing 19
Synonyms: 1500041L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 72008
Homologene: 12435
Brip1
Name: BRCA1 interacting protein C-terminal helicase 1
Synonyms: BACH1, 8030460J03Rik, 3110009N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237911
Homologene: 32766
Robo4
Name: roundabout guidance receptor 4
Synonyms: Magic roundabout, 1200012D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 74144
Homologene: 10397
Slc35e3
Name: solute carrier family 35, member E3
Synonyms: 9330166G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 215436
VEGA: 10
Homologene: 41279
Wif1
Name: Wnt inhibitory factor 1
Synonyms: WIF-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 24117
Homologene: 31430
Akr1c18
Name: aldo-keto reductase family 1, member C18
Synonyms: 20alpha-hydroxysteroid dehydrogenase, 20alpha-HSD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 105349
HGNC: HGNC:386
Homologene: 128661
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Ttn
Name: titin
Synonyms: D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, mdm, 1100001C23Rik, shru, connectin, L56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Pico, Acz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 26875
Homologene: 69111
Slc16a12
Name: solute carrier family 16 (monocarboxylic acid transporters), member 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 240638
Homologene: 130007
Lysmd4
Name: LysM, putative peptidoglycan-binding, domain containing 4
Synonyms: 4930506D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 75099
Homologene: 45123
Ciz1
Name: CDKN1A interacting zinc finger protein 1
Synonyms: 2900056O04Rik, 0610038H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 68379
Homologene: 8112
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380698
Homologene: 70869
Wnk2
Name: WNK lysine deficient protein kinase 2
Synonyms: ESTM15, 1810073P09Rik, X83337
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 75607
Homologene: 19155
Farp1
Name: FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived)
Synonyms: Cdep
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 223254
HGNC: HGNC:3591
Homologene: 38098
Scn9a
Name: sodium channel, voltage-gated, type IX, alpha
Synonyms: PN1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20274
Homologene: 2237
Atp11b
Name: ATPase, class VI, type 11B
Synonyms: 1110019I14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 76295
Homologene: 32919
Egflam
Name: EGF-like, fibronectin type III and laminin G domains
Synonyms: pikachurin, nectican
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 268780
Homologene: 65044
Umodl1
Name: uromodulin-like 1
Synonyms: D17Ertd488e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 52020
VEGA: 17
Homologene: 45466
Atg9a
Name: autophagy related 9A
Synonyms: Apg9l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 245860
Homologene: 34495
Gtf3c5
Name: general transcription factor IIIC, polypeptide 5
Synonyms: TFIIICepsilon, 2700084A09Rik, TFIIIC63, TFiiiC2-63
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 70239
HGNC: HGNC:4668
Homologene: 40806
Tmed6
Name: transmembrane p24 trafficking protein 6
Synonyms: 1810018I24Rik, 1810015P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 66269
Homologene: 11938
Spag16
Name: sperm associated antigen 16
Synonyms: Wdr29, 4930585K05Rik, 4930524F24Rik, Pf20, 4921511D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 66722
Homologene: 11572
Olfr967
Name: olfactory receptor 967
Synonyms: GA_x6K02T2PVTD-33447884-33448816, MOR171-30P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258086
VEGA: 9
HGNC: HGNC:8484
Homologene: 115535
Zfp217
Name: zinc finger protein 217
Synonyms: 4933431C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228913
Homologene: 4757
4933427D14Rik
Name: RIKEN cDNA 4933427D14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74477
Homologene: 8874
Bmp3
Name: bone morphogenetic protein 3
Synonyms: 9530029I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 110075
HGNC: HGNC:1070
Homologene: 927
Ercc6l2
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6 like 2
Synonyms: 0610007P08Rik, 1700019D06Rik, 9330134C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 76251
Homologene: 32564
Scaf11
Name: SR-related CTD-associated factor 11
Synonyms: 2610510E10Rik, Sfrs2ip, CASP11, SIP1, SRRP129, 1110061H03Rik, Srsf2ip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 72193
VEGA: 15
Homologene: 37957
Tsen54
Name: tRNA splicing endonuclease subunit 54
Synonyms: 0610034P02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76265
Homologene: 35476
Mex3b
Name: mex3 RNA binding family member B
Synonyms: Rkhd3, 4931439A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 108797
Homologene: 13570
Olfr1389
Name: olfactory receptor 1389
Synonyms: MOR256-1, GA_x6K02T2QP88-6004387-6003452
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 259069
Homologene: 86690
Zfp874b
Name: zinc finger protein 874b
Synonyms: 9630025I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 408067
Homologene: 135597
Olfr1219
Name: olfactory receptor 1219
Synonyms: GA_x6K02T2Q125-50555603-50554668, MOR233-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258901
Homologene: 27292
Ccdc185
Name: coiled-coil domain containing 185
Synonyms: 4922505E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 433386
Homologene: 17631
Mapk8ip2
Name: mitogen-activated protein kinase 8 interacting protein 2
Synonyms: IB2, 3230402N03Rik, Jip2, JNK-interacting protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 60597
HGNC: HGNC:6883
Homologene: 8201
Dagla
Name: diacylglycerol lipase, alpha
Synonyms: Nsddr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 269060
HGNC: HGNC:1165
Homologene: 4468
Adgra1
Name: adhesion G protein-coupled receptor A1
Synonyms: D7Ertd680e, 2900059M17Rik, Gpr123
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 52389
Homologene: 18582
Casd1
Name: CAS1 domain containing 1
Synonyms: Cast1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 213819
Homologene: 11287
Klk1b7-ps
Name: kallikrein 1-related peptidase b7, pseudogene
Synonyms: Klk-ps2, mGK-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16604
Clstn2
Name: calsyntenin 2
Synonyms: CS2, Cst-2, CSTN2, 2900042C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 64085
Homologene: 49698
Slc6a20b
Name: solute carrier family 6 (neurotransmitter transporter), member 20B
Synonyms: XT3, Xtrp3, Slc6a20, Sit1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 22599
Homologene: 130652
Zfp72
Name: zinc finger protein 72
Synonyms: Zfp74
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 238722
VEGA: 13
Homologene: 108290
Lats2
Name: large tumor suppressor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 50523
HGNC: HGNC:6515
Homologene: 56678
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 69,870,345 bp
  • C to A, chromosome 1 at 75,190,328 bp
  • A to G, chromosome 1 at 136,948,805 bp
  • T to A, chromosome 1 at 182,747,564 bp
  • T to C, chromosome 1 at 189,659,986 bp
  • T to C, chromosome 1 at 193,340,074 bp
  • A to G, chromosome 2 at 13,224,440 bp
  • A to T, chromosome 2 at 28,577,996 bp
  • T to C, chromosome 2 at 32,372,406 bp
  • A to T, chromosome 2 at 66,533,377 bp
  • A to G, chromosome 2 at 76,729,534 bp
  • A to G, chromosome 2 at 89,074,464 bp
  • A to G, chromosome 2 at 119,211,215 bp
  • C to T, chromosome 2 at 170,115,462 bp
  • A to G, chromosome 3 at 35,839,194 bp
  • G to A, chromosome 3 at 102,113,166 bp
  • T to A, chromosome 5 at 14,682,255 bp
  • T to C, chromosome 5 at 21,913,230 bp
  • A to G, chromosome 5 at 98,872,111 bp
  • A to G, chromosome 5 at 121,772,778 bp
  • A to G, chromosome 5 at 124,386,892 bp
  • A to T, chromosome 6 at 4,608,075 bp
  • G to A, chromosome 7 at 29,792,753 bp
  • A to G, chromosome 7 at 43,945,828 bp
  • A to T, chromosome 7 at 65,314,755 bp
  • T to A, chromosome 7 at 67,226,040 bp
  • A to G, chromosome 7 at 82,869,034 bp
  • A to G, chromosome 7 at 139,876,147 bp
  • A to T, chromosome 8 at 107,065,651 bp
  • A to T, chromosome 8 at 108,946,808 bp
  • A to T, chromosome 9 at 37,408,081 bp
  • A to G, chromosome 9 at 39,750,638 bp
  • A to G, chromosome 9 at 59,861,519 bp
  • A to C, chromosome 9 at 59,924,991 bp
  • A to T, chromosome 9 at 86,451,990 bp
  • A to T, chromosome 9 at 97,458,204 bp
  • A to G, chromosome 9 at 123,597,312 bp
  • C to A, chromosome 10 at 117,740,806 bp
  • G to T, chromosome 10 at 121,099,799 bp
  • A to T, chromosome 11 at 29,707,256 bp
  • C to A, chromosome 11 at 49,431,251 bp
  • C to A, chromosome 11 at 59,007,708 bp
  • T to A, chromosome 11 at 72,175,545 bp
  • A to G, chromosome 11 at 77,578,724 bp
  • G to T, chromosome 11 at 78,226,015 bp
  • T to C, chromosome 11 at 86,152,658 bp
  • A to T, chromosome 11 at 89,060,169 bp
  • A to T, chromosome 11 at 115,815,061 bp
  • G to A, chromosome 11 at 120,469,078 bp
  • A to G, chromosome 12 at 85,476,016 bp
  • A to T, chromosome 13 at 4,145,308 bp
  • T to C, chromosome 13 at 43,425,027 bp
  • A to G, chromosome 13 at 49,057,016 bp
  • T to A, chromosome 13 at 63,869,016 bp
  • A to G, chromosome 13 at 67,474,933 bp
  • T to C, chromosome 13 at 74,372,071 bp
  • G to A, chromosome 14 at 57,700,196 bp
  • T to C, chromosome 14 at 121,233,846 bp
  • A to G, chromosome 15 at 7,250,028 bp
  • T to C, chromosome 15 at 89,458,732 bp
  • G to T, chromosome 15 at 96,418,641 bp
  • C to A, chromosome 15 at 99,423,246 bp
  • C to A, chromosome 17 at 30,984,028 bp
  • A to G, chromosome 19 at 10,248,425 bp
  • C to A, chromosome 19 at 34,680,402 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0653 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038838-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.