Strain Name:
C57BL/6J-MtgxR1183Btlr/Mmmh
Stock Number:
039255-MU
Citation ID:
RRID:MMRRC_039255-MU
Other Names:
R1183 (G1), C57BL/6J-MtgxR1183Btlr
Major Collection:

Gene Information

Gene Symbol: Nfatc2 [MGI:102463] (Mus musculus (mouse))
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1-D, NFAT1, NFATp
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ap4e1 [MGI:1336993] (Mus musculus (mouse))
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Trim32 [MGI:1917057] (Mus musculus (mouse))
Name: tripartite motif-containing 32
Synonyms: 1810045E12Rik, 3f3, BBS11, Zfp117
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Fn1 [MGI:95566] (Mus musculus (mouse))
Name: fibronectin 1
Synonyms: Fn-1, Fn
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: S100a1 [MGI:1338917] (Mus musculus (mouse))
Name: S100 calcium binding protein A1
Synonyms: S100, S100a
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Rbpms [MGI:1334446] (Mus musculus (mouse))
Name: RNA binding protein gene with multiple splicing
Synonyms: hermes, 2010300K22Rik, 2700019M19Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Ubr5 [MGI:1918040] (Mus musculus (mouse))
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: Edd1, 4432411E13Rik, Edd
Chromosome: 15
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 71,586,245 bp
  • A to T, chromosome 1 at 88,348,091 bp
  • T to C, chromosome 2 at 27,084,080 bp
  • T to A, chromosome 2 at 29,180,092 bp
  • T to C, chromosome 2 at 31,011,785 bp
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • A to G, chromosome 2 at 72,940,746 bp
  • A to T, chromosome 2 at 91,477,519 bp
  • T to A, chromosome 2 at 91,586,266 bp
  • A to T, chromosome 2 at 125,321,617 bp
  • T to A, chromosome 2 at 127,014,201 bp
  • C to A, chromosome 2 at 127,465,065 bp
  • T to A, chromosome 2 at 130,247,976 bp
  • C to T, chromosome 2 at 153,261,238 bp
  • A to G, chromosome 2 at 168,231,653 bp
  • T to C, chromosome 2 at 168,590,088 bp
  • T to A, chromosome 3 at 36,895,303 bp
  • T to A, chromosome 3 at 90,159,945 bp
  • C to T, chromosome 3 at 90,511,334 bp
  • A to T, chromosome 4 at 48,421,725 bp
  • A to G, chromosome 4 at 65,614,391 bp
  • T to C, chromosome 4 at 114,907,465 bp
  • A to T, chromosome 4 at 126,925,839 bp
  • T to C, chromosome 4 at 129,357,727 bp
  • A to G, chromosome 4 at 134,529,125 bp
  • A to G, chromosome 5 at 3,643,707 bp
  • T to C, chromosome 5 at 30,371,912 bp
  • T to A, chromosome 5 at 65,034,388 bp
  • T to C, chromosome 5 at 74,595,102 bp
  • C to T, chromosome 5 at 75,946,851 bp
  • T to C, chromosome 5 at 125,023,521 bp
  • T to C, chromosome 6 at 119,320,885 bp
  • T to A, chromosome 6 at 124,200,532 bp
  • A to T, chromosome 7 at 22,843,594 bp
  • T to C, chromosome 7 at 35,794,890 bp
  • T to C, chromosome 7 at 45,985,253 bp
  • G to A, chromosome 7 at 46,289,755 bp
  • G to T, chromosome 7 at 46,889,624 bp
  • A to G, chromosome 7 at 108,893,741 bp
  • A to G, chromosome 7 at 127,406,247 bp
  • T to A, chromosome 8 at 27,114,388 bp
  • A to G, chromosome 8 at 33,574,865 bp
  • C to A, chromosome 8 at 33,804,072 bp
  • A to G, chromosome 8 at 70,631,552 bp
  • A to T, chromosome 8 at 71,684,550 bp
  • A to G, chromosome 8 at 84,580,217 bp
  • T to G, chromosome 8 at 85,780,523 bp
  • A to G, chromosome 8 at 92,889,405 bp
  • A to C, chromosome 8 at 93,268,005 bp
  • T to C, chromosome 8 at 105,382,966 bp
  • T to G, chromosome 8 at 111,041,574 bp
  • C to A, chromosome 9 at 37,408,052 bp
  • T to C, chromosome 9 at 44,139,388 bp
  • T to A, chromosome 9 at 50,789,533 bp
  • T to C, chromosome 9 at 65,121,900 bp
  • A to T, chromosome 9 at 74,179,585 bp
  • A to G, chromosome 10 at 77,923,564 bp
  • A to T, chromosome 10 at 93,870,184 bp
  • A to G, chromosome 10 at 117,292,810 bp
  • A to G, chromosome 11 at 6,674,867 bp
  • T to C, chromosome 11 at 19,009,262 bp
  • T to C, chromosome 11 at 56,033,037 bp
  • T to C, chromosome 11 at 69,446,648 bp
  • T to C, chromosome 11 at 75,490,330 bp
  • T to C, chromosome 11 at 77,857,745 bp
  • T to C, chromosome 11 at 101,421,905 bp
  • T to C, chromosome 12 at 91,325,598 bp
  • T to C, chromosome 12 at 98,792,046 bp
  • G to A, chromosome 13 at 38,191,740 bp
  • T to G, chromosome 13 at 41,828,337 bp
  • C to T, chromosome 13 at 49,205,251 bp
  • T to C, chromosome 13 at 95,701,113 bp
  • A to G, chromosome 13 at 110,353,838 bp
  • C to A, chromosome 14 at 64,782,377 bp
  • T to A, chromosome 14 at 124,676,524 bp
  • T to C, chromosome 15 at 37,997,175 bp
  • A to G, chromosome 15 at 58,971,048 bp
  • G to A, chromosome 15 at 75,918,798 bp
  • A to T, chromosome 15 at 79,728,468 bp
  • T to C, chromosome 16 at 5,064,542 bp
  • T to C, chromosome 16 at 22,841,769 bp
  • T to A, chromosome 17 at 23,900,494 bp
  • T to C, chromosome 17 at 34,418,850 bp
  • T to A, chromosome 17 at 35,929,443 bp
  • A to G, chromosome 18 at 20,283,198 bp
  • G to T, chromosome 18 at 24,271,590 bp
  • A to G, chromosome 18 at 63,086,753 bp
  • A to G, chromosome 18 at 77,960,711 bp
  • T to C, chromosome 19 at 41,954,831 bp
  • T to C, chromosome 19 at 57,650,293 bp
  • T to A, chromosome 19 at 58,512,710 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1183 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039255-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.