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Strain Name:
C57BL/6J-MtgxR1464Btlr/Mmmh
Stock Number:
039518-MU
Citation ID:
RRID:MMRRC_039518-MU
Other Names:
R1464 (G1), C57BL/6J-MtgxR1464Btlr
Major Collection:

Strain Information

Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Mthfr
Name: methylenetetrahydrofolate reductase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17769
HGNC: HGNC:7436
Homologene: 4349
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Macroh2a1
Name: macroH2A.1 histone
Synonyms: mH2a1, MACROH2A1.2, H2AF12M, H2afy
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26914
HGNC: HGNC:4740
Homologene: 3598
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 38,865,691 bp
  • G to A, chromosome 1 at 43,764,916 bp
  • A to G, chromosome 1 at 60,125,906 bp
  • A to T, chromosome 1 at 87,698,105 bp
  • A to G, chromosome 1 at 136,156,153 bp
  • C to T, chromosome 1 at 153,850,531 bp
  • A to G, chromosome 1 at 162,794,355 bp
  • A to G, chromosome 1 at 182,748,698 bp
  • G to T, chromosome 2 at 13,325,288 bp
  • T to G, chromosome 2 at 25,447,834 bp
  • T to A, chromosome 2 at 58,906,362 bp
  • T to C, chromosome 2 at 58,934,443 bp
  • T to A, chromosome 2 at 76,758,994 bp
  • A to G, chromosome 2 at 111,225,403 bp
  • T to A, chromosome 2 at 120,021,261 bp
  • T to C, chromosome 2 at 137,115,648 bp
  • A to T, chromosome 2 at 139,547,234 bp
  • T to A, chromosome 2 at 164,698,648 bp
  • T to C, chromosome 2 at 181,239,654 bp
  • A to G, chromosome 3 at 27,440,185 bp
  • T to A, chromosome 3 at 32,461,841 bp
  • A to G, chromosome 3 at 138,288,747 bp
  • A to T, chromosome 3 at 145,853,274 bp
  • T to A, chromosome 4 at 46,606,491 bp
  • T to C, chromosome 4 at 58,000,274 bp
  • T to C, chromosome 4 at 83,011,879 bp
  • G to T, chromosome 4 at 88,842,000 bp
  • T to A, chromosome 4 at 101,735,681 bp
  • T to A, chromosome 4 at 101,941,306 bp
  • A to T, chromosome 4 at 126,792,031 bp
  • C to A, chromosome 4 at 129,002,359 bp
  • A to G, chromosome 4 at 139,370,937 bp
  • C to A, chromosome 4 at 140,034,525 bp
  • T to C, chromosome 4 at 148,000,847 bp
  • T to A, chromosome 4 at 148,053,572 bp
  • A to T, chromosome 5 at 21,483,991 bp
  • G to A, chromosome 5 at 24,413,821 bp
  • A to G, chromosome 5 at 43,609,139 bp
  • A to G, chromosome 5 at 73,635,553 bp
  • C to A, chromosome 5 at 122,714,539 bp
  • T to G, chromosome 5 at 137,419,929 bp
  • T to A, chromosome 5 at 137,642,360 bp
  • T to C, chromosome 5 at 137,905,565 bp
  • T to A, chromosome 6 at 58,265,232 bp
  • C to T, chromosome 6 at 88,376,200 bp
  • G to A, chromosome 6 at 90,223,932 bp
  • A to G, chromosome 6 at 91,053,569 bp
  • A to G, chromosome 6 at 100,866,566 bp
  • T to C, chromosome 6 at 113,294,737 bp
  • A to T, chromosome 6 at 119,361,843 bp
  • A to G, chromosome 6 at 122,737,310 bp
  • C to T, chromosome 6 at 135,228,492 bp
  • C to T, chromosome 6 at 141,992,517 bp
  • A to T, chromosome 7 at 5,035,801 bp
  • T to C, chromosome 7 at 23,505,256 bp
  • A to G, chromosome 7 at 24,260,548 bp
  • T to C, chromosome 7 at 49,362,204 bp
  • T to A, chromosome 7 at 64,695,549 bp
  • T to C, chromosome 7 at 91,968,198 bp
  • A to G, chromosome 7 at 102,031,575 bp
  • G to A, chromosome 7 at 102,114,247 bp
  • G to T, chromosome 7 at 140,419,373 bp
  • A to G, chromosome 8 at 109,636,427 bp
  • C to T, chromosome 8 at 119,579,310 bp
  • T to C, chromosome 8 at 122,892,724 bp
  • T to A, chromosome 9 at 30,951,377 bp
  • T to A, chromosome 9 at 51,057,586 bp
  • A to G, chromosome 9 at 58,260,426 bp
  • T to A, chromosome 9 at 63,140,111 bp
  • T to C, chromosome 9 at 104,031,879 bp
  • T to C, chromosome 9 at 104,214,167 bp
  • A to T, chromosome 9 at 110,840,951 bp
  • A to G, chromosome 10 at 41,455,079 bp
  • A to G, chromosome 10 at 88,445,754 bp
  • T to A, chromosome 10 at 128,048,288 bp
  • T to A, chromosome 11 at 53,372,524 bp
  • A to G, chromosome 11 at 60,539,047 bp
  • T to A, chromosome 11 at 98,516,905 bp
  • A to C, chromosome 11 at 106,784,885 bp
  • G to A, chromosome 11 at 113,830,080 bp
  • A to G, chromosome 11 at 119,272,984 bp
  • A to G, chromosome 12 at 31,184,915 bp
  • C to A, chromosome 12 at 69,805,350 bp
  • T to A, chromosome 12 at 78,612,964 bp
  • A to T, chromosome 13 at 22,279,178 bp
  • G to T, chromosome 13 at 44,848,381 bp
  • G to A, chromosome 13 at 49,081,975 bp
  • G to A, chromosome 13 at 49,676,788 bp
  • A to T, chromosome 13 at 56,083,136 bp
  • T to C, chromosome 13 at 111,755,871 bp
  • A to G, chromosome 14 at 56,461,911 bp
  • T to G, chromosome 15 at 12,146,372 bp
  • C to T, chromosome 15 at 35,709,484 bp
  • C to T, chromosome 15 at 54,863,812 bp
  • A to G, chromosome 15 at 79,485,404 bp
  • T to A, chromosome 15 at 84,804,959 bp
  • A to G, chromosome 16 at 20,596,269 bp
  • A to G, chromosome 16 at 33,229,085 bp
  • T to A, chromosome 16 at 96,801,253 bp
  • G to A, chromosome 17 at 20,228,742 bp
  • A to T, chromosome 17 at 24,181,223 bp
  • C to A, chromosome 17 at 26,839,279 bp
  • C to A, chromosome 17 at 30,695,173 bp
  • A to T, chromosome 17 at 31,473,162 bp
  • G to T, chromosome 17 at 46,572,591 bp
  • T to G, chromosome 17 at 53,515,987 bp
  • C to T, chromosome 18 at 12,914,558 bp
  • A to G, chromosome 18 at 35,680,847 bp
  • A to G, chromosome 18 at 60,538,406 bp
  • T to C, chromosome 19 at 5,879,900 bp
  • G to T, chromosome 19 at 9,004,896 bp
  • T to C, chromosome 19 at 29,382,592 bp
  • A to G, chromosome 19 at 30,578,870 bp
  • C to A, chromosome 19 at 38,403,217 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1464 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039518-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.