Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR1614Btlr/Mmmh
Stock Number:
039651-MU
Citation ID:
RRID:MMRRC_039651-MU
Other Names:
R1614 (G1), C57BL/6J-MtgxR1614Btlr
Major Collection:

Strain Information

Slc17a6
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: 2900073D12Rik, VGLUT2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 140919
Homologene: 121617
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Ccdc88c
Name: coiled-coil domain containing 88C
Synonyms: Daple, 0610010D24Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68339
VEGA: 12
Homologene: 18903
Pecam1
Name: platelet/endothelial cell adhesion molecule 1
Synonyms: PECAM-1, Cd31
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18613
HGNC: HGNC:8823
Homologene: 47925
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Ppp2r5e
Name: protein phosphatase 2, regulatory subunit B', epsilon
Synonyms: protein phosphatase 2A subunit beta, 4633401M22Rik, B56beta
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26932
VEGA: 12
HGNC: HGNC:9313
Homologene: 55962
Pop1
Name: processing of precursor 1, ribonuclease P/MRP family, (S. cerevisiae)
Synonyms: 4932434G09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67724
Homologene: 41000
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,275,263 bp
  • T to C, chromosome 1 at 51,471,352 bp
  • G to A, chromosome 1 at 60,501,746 bp
  • T to C, chromosome 1 at 74,685,980 bp
  • A to G, chromosome 1 at 139,463,310 bp
  • T to C, chromosome 1 at 177,447,170 bp
  • G to T, chromosome 2 at 24,690,807 bp
  • T to G, chromosome 2 at 36,688,309 bp
  • G to T, chromosome 2 at 62,850,604 bp
  • T to A, chromosome 2 at 89,590,696 bp
  • T to A, chromosome 2 at 111,293,066 bp
  • T to A, chromosome 2 at 112,156,517 bp
  • T to C, chromosome 2 at 120,697,675 bp
  • G to T, chromosome 2 at 146,388,612 bp
  • A to C, chromosome 3 at 151,801,779 bp
  • C to T, chromosome 4 at 99,061,280 bp
  • A to T, chromosome 4 at 103,026,649 bp
  • A to T, chromosome 4 at 115,796,757 bp
  • A to G, chromosome 4 at 117,904,618 bp
  • A to G, chromosome 5 at 24,594,876 bp
  • A to G, chromosome 5 at 77,010,565 bp
  • T to A, chromosome 6 at 58,940,184 bp
  • T to G, chromosome 6 at 124,223,934 bp
  • A to G, chromosome 7 at 15,945,965 bp
  • G to T, chromosome 7 at 24,126,599 bp
  • A to T, chromosome 7 at 49,826,719 bp
  • G to A, chromosome 7 at 51,646,277 bp
  • T to C, chromosome 7 at 79,811,619 bp
  • A to T, chromosome 7 at 85,221,738 bp
  • G to A, chromosome 8 at 13,066,904 bp
  • G to A, chromosome 8 at 128,720,065 bp
  • T to C, chromosome 9 at 87,212,932 bp
  • T to A, chromosome 9 at 105,467,826 bp
  • A to G, chromosome 10 at 88,414,589 bp
  • T to C, chromosome 11 at 69,743,373 bp
  • T to G, chromosome 11 at 85,172,864 bp
  • C to T, chromosome 11 at 87,731,659 bp
  • T to C, chromosome 11 at 90,683,254 bp
  • T to C, chromosome 11 at 105,349,555 bp
  • A to C, chromosome 11 at 106,168,319 bp
  • T to C, chromosome 11 at 106,681,079 bp
  • A to T, chromosome 11 at 115,616,623 bp
  • A to G, chromosome 12 at 16,701,171 bp
  • C to G, chromosome 12 at 75,469,567 bp
  • A to T, chromosome 12 at 100,912,984 bp
  • C to T, chromosome 13 at 55,069,817 bp
  • A to G, chromosome 13 at 86,045,785 bp
  • C to A, chromosome 15 at 34,530,210 bp
  • G to A, chromosome 15 at 45,114,444 bp
  • A to G, chromosome 17 at 25,727,090 bp
  • A to T, chromosome 17 at 33,242,332 bp
  • T to A, chromosome 17 at 91,236,996 bp
  • G to A, chromosome 19 at 17,515,256 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1614 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039651-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.