Strain Name:
C57BL/6J-MtgxR1623Btlr/Mmmh
Stock Number:
039660-MU
Citation ID:
RRID:MMRRC_039660-MU
Other Names:
R1623 (G1), C57BL/6J-MtgxR1623Btlr
Major Collection:

Gene Information

Gene Symbol: Pkd1 [MGI:97603] (Mus musculus (mouse))
Name: polycystin 1, transient receptor poteintial channel interacting
Synonyms: polycystin-1, PC1, PC-1
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Ppp2r2d [MGI:1289252] (Mus musculus (mouse))
Name: protein phosphatase 2, regulatory subunit B, delta
Synonyms: MDS026, 1300017E19Rik, D7Ertd753e
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Derl2 [MGI:2151483] (Mus musculus (mouse))
Name: Der1-like domain family, member 2
Synonyms: Derlin-2, Flana, F-lana, CGI-101
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Notch3 [MGI:99460] (Mus musculus (mouse))
Name: notch 3
Synonyms: N3, hpbk
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Kmt2e [MGI:1924825] (Mus musculus (mouse))
Name: lysine (K)-specific methyltransferase 2E
Synonyms: Mll5, 1810033J14Rik, D230038D11Rik, 9530077A04Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Sptan1 [MGI:98386] (Mus musculus (mouse))
Name: spectrin alpha, non-erythrocytic 1
Synonyms: 2610027H02Rik, alpha-fodrin, Spna2, alphaII-spectrin, Spna-2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Zfp113 [MGI:1929116] (Mus musculus (mouse))
Name: zinc finger protein 113
Synonyms: 4732456B05Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,081,296 bp
  • A to G, chromosome 1 at 36,644,324 bp
  • T to C, chromosome 1 at 74,571,775 bp
  • A to T, chromosome 1 at 93,423,869 bp
  • T to A, chromosome 1 at 128,272,851 bp
  • A to G, chromosome 1 at 164,195,622 bp
  • A to T, chromosome 1 at 174,137,317 bp
  • A to C, chromosome 1 at 174,368,949 bp
  • A to T, chromosome 2 at 23,094,344 bp
  • T to C, chromosome 2 at 25,052,599 bp
  • G to A, chromosome 2 at 26,478,612 bp
  • A to T, chromosome 2 at 28,504,976 bp
  • A to G, chromosome 2 at 29,986,420 bp
  • A to T, chromosome 2 at 31,458,039 bp
  • C to T, chromosome 2 at 69,977,723 bp
  • T to C, chromosome 2 at 91,487,033 bp
  • T to A, chromosome 2 at 104,152,963 bp
  • T to A, chromosome 2 at 121,562,986 bp
  • T to C, chromosome 2 at 127,141,635 bp
  • A to G, chromosome 2 at 173,154,718 bp
  • T to A, chromosome 2 at 176,807,364 bp
  • T to A, chromosome 3 at 54,299,179 bp
  • T to A, chromosome 3 at 54,734,331 bp
  • G to A, chromosome 3 at 105,716,820 bp
  • T to A, chromosome 3 at 107,967,835 bp
  • A to G, chromosome 3 at 130,550,050 bp
  • C to T, chromosome 3 at 132,724,766 bp
  • A to T, chromosome 3 at 144,814,060 bp
  • T to A, chromosome 4 at 3,585,964 bp
  • A to T, chromosome 4 at 19,824,031 bp
  • A to T, chromosome 4 at 111,980,099 bp
  • T to C, chromosome 4 at 120,095,704 bp
  • G to T, chromosome 5 at 13,796,321 bp
  • A to G, chromosome 5 at 23,482,502 bp
  • T to A, chromosome 5 at 86,926,408 bp
  • T to A, chromosome 5 at 123,029,202 bp
  • T to C, chromosome 5 at 137,871,248 bp
  • T to C, chromosome 5 at 138,145,668 bp
  • T to A, chromosome 5 at 143,701,372 bp
  • C to T, chromosome 5 at 145,132,929 bp
  • T to C, chromosome 5 at 149,215,199 bp
  • T to A, chromosome 5 at 149,574,116 bp
  • C to A, chromosome 6 at 87,378,170 bp
  • C to T, chromosome 6 at 88,485,770 bp
  • C to T, chromosome 6 at 112,628,519 bp
  • C to T, chromosome 6 at 121,024,807 bp
  • C to T, chromosome 7 at 29,095,490 bp
  • A to T, chromosome 7 at 42,135,855 bp
  • C to T, chromosome 7 at 89,886,182 bp
  • A to G, chromosome 7 at 101,289,906 bp
  • A to G, chromosome 7 at 110,264,048 bp
  • A to T, chromosome 7 at 135,708,818 bp
  • T to C, chromosome 7 at 138,872,990 bp
  • A to G, chromosome 7 at 141,273,937 bp
  • A to G, chromosome 8 at 84,133,271 bp
  • A to G, chromosome 8 at 125,877,439 bp
  • A to G, chromosome 8 at 126,953,236 bp
  • A to G, chromosome 9 at 40,782,560 bp
  • A to G, chromosome 10 at 33,258,102 bp
  • T to G, chromosome 10 at 41,481,393 bp
  • A to G, chromosome 10 at 59,428,239 bp
  • C to G, chromosome 10 at 93,516,297 bp
  • A to T, chromosome 10 at 100,538,917 bp
  • C to A, chromosome 10 at 127,790,853 bp
  • T to A, chromosome 11 at 43,201,957 bp
  • C to A, chromosome 11 at 50,668,115 bp
  • T to C, chromosome 11 at 66,037,637 bp
  • C to T, chromosome 11 at 69,021,142 bp
  • T to C, chromosome 11 at 69,035,762 bp
  • T to C, chromosome 11 at 70,618,428 bp
  • T to C, chromosome 11 at 71,019,105 bp
  • T to C, chromosome 11 at 78,262,834 bp
  • C to T, chromosome 11 at 98,490,331 bp
  • C to A, chromosome 11 at 115,991,316 bp
  • T to C, chromosome 11 at 120,009,269 bp
  • A to G, chromosome 12 at 16,674,770 bp
  • T to A, chromosome 12 at 103,316,332 bp
  • A to T, chromosome 13 at 12,340,439 bp
  • A to T, chromosome 13 at 19,751,912 bp
  • T to C, chromosome 13 at 33,029,565 bp
  • G to A, chromosome 13 at 114,824,622 bp
  • T to C, chromosome 14 at 59,287,551 bp
  • G to T, chromosome 15 at 66,891,599 bp
  • A to G, chromosome 15 at 76,638,509 bp
  • A to G, chromosome 15 at 85,867,578 bp
  • G to A, chromosome 15 at 97,808,404 bp
  • G to A, chromosome 16 at 44,951,448 bp
  • A to G, chromosome 16 at 59,483,042 bp
  • G to A, chromosome 17 at 24,578,269 bp
  • G to A, chromosome 17 at 25,947,534 bp
  • G to T, chromosome 17 at 29,687,047 bp
  • T to C, chromosome 17 at 32,139,191 bp
  • C to T, chromosome 17 at 80,556,302 bp
  • T to A, chromosome 18 at 6,620,091 bp
  • T to C, chromosome 18 at 20,275,177 bp
  • T to C, chromosome 18 at 32,000,051 bp
  • A to G, chromosome 18 at 50,179,037 bp
  • C to A, chromosome 18 at 56,432,351 bp
  • G to T, chromosome 18 at 58,048,548 bp
  • A to G, chromosome 18 at 66,859,997 bp
  • T to A, chromosome 19 at 10,646,744 bp
  • A to G, chromosome 19 at 12,804,094 bp
  • T to A, chromosome 19 at 28,904,722 bp
  • A to G, chromosome 19 at 44,569,423 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1623 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039660-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.