Strain Name:
C57BL/6J-MtgxR1750Btlr/Mmmh
Stock Number:
039782-MU
Citation ID:
RRID:MMRRC_039782-MU
Other Names:
R1750 (G1), C57BL/6J-MtgxR1750Btlr
Major Collection:

Gene Information

Gene Symbol: Chrna3 [MGI:87887] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, alpha polypeptide 3
Synonyms: A730007P14Rik, Acra3, neuronal nicotinic acetylcholine receptor, alpha 3 subunit, alpha 3, (a)3, Acra-3
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Inpp5d [MGI:107357] (Mus musculus (mouse))
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP-1, SHIP, s-SHIP, Src homology 2 domain-containing inositol-5-phosphatase, SHIP1
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Cacna1g [MGI:1201678] (Mus musculus (mouse))
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: Cav3.1d, a1G
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Rgs7 [MGI:1346089] (Mus musculus (mouse))
Name: regulator of G protein signaling 7
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Prok2 [MGI:1354178] (Mus musculus (mouse))
Name: prokineticin 2
Synonyms: PK2, Bombina variegata 8kDa protein, Bv8
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Becn1 [MGI:1891828] (Mus musculus (mouse))
Name: beclin 1, autophagy related
Synonyms: Atg6
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Epb41l4a [MGI:103007] (Mus musculus (mouse))
Name: erythrocyte membrane protein band 4.1 like 4a
Synonyms: Epb4.1l4a, NBL4
Chromosome: 18
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 38,995,865 bp
  • T to C, chromosome 1 at 63,719,522 bp
  • C to T, chromosome 1 at 72,325,087 bp
  • T to C, chromosome 1 at 87,699,081 bp
  • C to A, chromosome 1 at 131,844,064 bp
  • T to A, chromosome 1 at 132,507,583 bp
  • T to C, chromosome 1 at 134,079,031 bp
  • C to A, chromosome 1 at 158,763,150 bp
  • T to C, chromosome 1 at 175,086,106 bp
  • T to A, chromosome 1 at 177,447,511 bp
  • T to A, chromosome 1 at 182,488,982 bp
  • A to T, chromosome 2 at 23,146,081 bp
  • C to T, chromosome 2 at 75,980,255 bp
  • T to C, chromosome 2 at 87,308,852 bp
  • T to A, chromosome 2 at 87,753,186 bp
  • C to A, chromosome 2 at 88,560,058 bp
  • T to C, chromosome 2 at 88,755,773 bp
  • T to C, chromosome 2 at 90,793,869 bp
  • T to A, chromosome 2 at 90,816,376 bp
  • T to C, chromosome 2 at 91,167,891 bp
  • T to A, chromosome 2 at 137,046,749 bp
  • T to C, chromosome 3 at 40,709,232 bp
  • G to T, chromosome 3 at 45,381,881 bp
  • A to T, chromosome 3 at 87,381,791 bp
  • A to G, chromosome 3 at 96,622,226 bp
  • A to G, chromosome 3 at 116,546,294 bp
  • A to G, chromosome 3 at 141,827,517 bp
  • A to G, chromosome 3 at 142,635,989 bp
  • A to T, chromosome 3 at 152,744,464 bp
  • A to T, chromosome 3 at 152,846,321 bp
  • G to C, chromosome 4 at 46,606,519 bp
  • G to A, chromosome 4 at 49,451,042 bp
  • A to G, chromosome 4 at 63,972,735 bp
  • A to G, chromosome 4 at 134,362,688 bp
  • T to A, chromosome 4 at 155,628,680 bp
  • C to T, chromosome 5 at 16,264,288 bp
  • T to C, chromosome 5 at 30,257,773 bp
  • G to A, chromosome 5 at 31,206,216 bp
  • G to T, chromosome 5 at 34,000,786 bp
  • T to C, chromosome 5 at 66,019,310 bp
  • A to G, chromosome 5 at 76,857,675 bp
  • A to G, chromosome 5 at 88,503,227 bp
  • T to A, chromosome 5 at 120,871,777 bp
  • T to C, chromosome 5 at 148,277,633 bp
  • A to G, chromosome 5 at 149,581,886 bp
  • A to T, chromosome 6 at 36,916,434 bp
  • A to T, chromosome 6 at 87,349,123 bp
  • A to G, chromosome 6 at 99,722,648 bp
  • A to G, chromosome 6 at 116,640,267 bp
  • A to G, chromosome 6 at 125,137,638 bp
  • A to C, chromosome 6 at 126,642,808 bp
  • A to G, chromosome 6 at 135,242,045 bp
  • T to G, chromosome 7 at 10,052,988 bp
  • T to C, chromosome 7 at 12,581,705 bp
  • T to A, chromosome 7 at 28,093,443 bp
  • A to T, chromosome 7 at 38,265,895 bp
  • T to A, chromosome 7 at 42,045,827 bp
  • T to C, chromosome 7 at 64,373,013 bp
  • T to A, chromosome 7 at 90,191,182 bp
  • G to A, chromosome 7 at 106,082,281 bp
  • T to G, chromosome 7 at 107,753,358 bp
  • A to T, chromosome 7 at 108,565,357 bp
  • G to T, chromosome 7 at 131,146,130 bp
  • A to T, chromosome 7 at 142,258,025 bp
  • A to T, chromosome 8 at 15,917,303 bp
  • A to T, chromosome 8 at 36,858,090 bp
  • T to G, chromosome 8 at 43,570,189 bp
  • A to G, chromosome 8 at 69,743,450 bp
  • C to A, chromosome 8 at 105,698,892 bp
  • T to A, chromosome 8 at 107,057,386 bp
  • C to A, chromosome 8 at 123,134,524 bp
  • T to A, chromosome 9 at 21,203,232 bp
  • T to A, chromosome 9 at 38,430,690 bp
  • C to T, chromosome 9 at 44,940,152 bp
  • T to C, chromosome 9 at 55,016,057 bp
  • A to T, chromosome 9 at 104,221,477 bp
  • A to T, chromosome 9 at 107,524,644 bp
  • C to A, chromosome 9 at 109,111,768 bp
  • G to A, chromosome 9 at 109,558,246 bp
  • A to T, chromosome 9 at 109,650,073 bp
  • G to T, chromosome 10 at 39,947,933 bp
  • A to C, chromosome 10 at 63,136,197 bp
  • A to G, chromosome 10 at 69,279,406 bp
  • C to A, chromosome 10 at 80,037,173 bp
  • AGCGCCTTGG to AG, chromosome 10 at 80,805,585 bp
  • A to G, chromosome 10 at 94,799,497 bp
  • T to C, chromosome 10 at 111,977,103 bp
  • A to T, chromosome 10 at 121,603,027 bp
  • A to G, chromosome 10 at 128,201,325 bp
  • G to T, chromosome 11 at 16,263,166 bp
  • A to T, chromosome 11 at 29,453,111 bp
  • T to C, chromosome 11 at 48,894,831 bp
  • A to C, chromosome 11 at 63,070,041 bp
  • A to T, chromosome 11 at 67,879,953 bp
  • A to T, chromosome 11 at 70,462,567 bp
  • A to T, chromosome 11 at 94,443,292 bp
  • C to G, chromosome 11 at 100,104,058 bp
  • C to T, chromosome 11 at 101,299,051 bp
  • G to T, chromosome 11 at 106,991,445 bp
  • A to G, chromosome 11 at 120,562,720 bp
  • T to C, chromosome 12 at 21,203,998 bp
  • A to T, chromosome 12 at 73,928,305 bp
  • T to A, chromosome 12 at 76,052,805 bp
  • T to A, chromosome 13 at 18,129,201 bp
  • T to G, chromosome 13 at 51,449,292 bp
  • C to A, chromosome 13 at 58,237,043 bp
  • T to A, chromosome 13 at 59,728,695 bp
  • C to A, chromosome 13 at 67,491,479 bp
  • T to A, chromosome 13 at 76,140,601 bp
  • A to T, chromosome 13 at 93,095,663 bp
  • T to C, chromosome 14 at 15,358,601 bp
  • C to T, chromosome 14 at 31,174,882 bp
  • T to A, chromosome 14 at 55,542,391 bp
  • C to T, chromosome 15 at 5,212,098 bp
  • A to G, chromosome 15 at 12,385,864 bp
  • G to A, chromosome 15 at 74,541,827 bp
  • A to T, chromosome 15 at 76,067,069 bp
  • T to A, chromosome 15 at 100,178,466 bp
  • T to C, chromosome 15 at 100,401,287 bp
  • A to G, chromosome 15 at 101,553,365 bp
  • G to A, chromosome 15 at 101,946,377 bp
  • T to A, chromosome 16 at 14,200,758 bp
  • T to C, chromosome 16 at 14,215,790 bp
  • T to G, chromosome 16 at 32,252,117 bp
  • T to A, chromosome 17 at 7,929,910 bp
  • C to T, chromosome 17 at 24,914,459 bp
  • G to A, chromosome 17 at 35,172,151 bp
  • T to A, chromosome 17 at 35,621,048 bp
  • A to C, chromosome 17 at 37,659,673 bp
  • G to C, chromosome 18 at 33,828,208 bp
  • C to T, chromosome 18 at 37,485,711 bp
  • A to G, chromosome 18 at 37,487,017 bp
  • T to A, chromosome 18 at 62,607,749 bp
  • C to A, chromosome 18 at 68,106,687 bp
  • A to G, chromosome 19 at 4,173,157 bp
  • G to A, chromosome 19 at 7,235,161 bp
  • G to A, chromosome 19 at 12,646,315 bp
  • A to T, chromosome X at 20,162,478 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1750 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039782-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.