Strain Name:
C57BL/6J-MtgxR1789Btlr/Mmmh
Stock Number:
039820-MU
Citation ID:
RRID:MMRRC_039820-MU
Other Names:
R1789 (G1), C57BL/6J-MtgxR1789Btlr
Major Collection:

Gene Information

Gene Symbol: Pitx2 [MGI:109340] (Mus musculus (mouse))
Name: paired-like homeodomain transcription factor 2
Synonyms: Pitx2a, Pitx2c, Brx1, Munc30, Rieg, Brx1a, Brx1b, Otlx2, solurshin, Pitx2b, Ptx2
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Myo10 [MGI:107716] (Mus musculus (mouse))
Name: myosin X
Synonyms: D15Ertd600e, myosin-X
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Notch3 [MGI:99460] (Mus musculus (mouse))
Name: notch 3
Synonyms: N3, hpbk
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Itgb8 [MGI:1338035] (Mus musculus (mouse))
Name: integrin beta 8
Synonyms: 4832412O06Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Chrna5 [MGI:87889] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra5, Acra-5
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Myo1e [MGI:106621] (Mus musculus (mouse))
Name: myosin IE
Synonyms: 2310020N23Rik, 9130023P14Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Arid5b [MGI:2175912] (Mus musculus (mouse))
Name: AT rich interactive domain 5B (MRF1-like)
Synonyms: Mrf2beta, 5430435G07Rik, Mrf2alpha, Desrt, Mrf2
Chromosome: 10
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 30,806,206 bp
  • A to G, chromosome 1 at 45,378,305 bp
  • T to C, chromosome 1 at 45,394,776 bp
  • A to T, chromosome 1 at 107,450,273 bp
  • A to T, chromosome 2 at 22,578,238 bp
  • T to G, chromosome 2 at 25,567,622 bp
  • T to C, chromosome 2 at 31,611,735 bp
  • T to C, chromosome 2 at 70,016,367 bp
  • T to A, chromosome 2 at 80,520,556 bp
  • G to T, chromosome 2 at 82,977,562 bp
  • A to T, chromosome 2 at 87,544,983 bp
  • C to T, chromosome 2 at 130,443,600 bp
  • T to C, chromosome 2 at 145,939,993 bp
  • C to A, chromosome 2 at 162,934,488 bp
  • A to G, chromosome 2 at 162,974,502 bp
  • T to C, chromosome 2 at 169,582,515 bp
  • T to G, chromosome 3 at 81,963,917 bp
  • C to T, chromosome 3 at 99,352,246 bp
  • T to A, chromosome 3 at 99,939,356 bp
  • T to A, chromosome 3 at 103,099,126 bp
  • C to A, chromosome 3 at 113,558,165 bp
  • C to T, chromosome 3 at 123,026,127 bp
  • T to A, chromosome 3 at 129,218,754 bp
  • T to G, chromosome 3 at 146,915,780 bp
  • C to A, chromosome 4 at 45,046,389 bp
  • A to G, chromosome 4 at 83,554,808 bp
  • T to A, chromosome 4 at 115,041,782 bp
  • T to A, chromosome 4 at 117,167,361 bp
  • G to A, chromosome 4 at 126,752,003 bp
  • T to C, chromosome 4 at 139,393,053 bp
  • A to T, chromosome 5 at 5,575,652 bp
  • T to A, chromosome 5 at 112,941,718 bp
  • A to T, chromosome 5 at 121,631,393 bp
  • G to A, chromosome 5 at 125,019,890 bp
  • T to G, chromosome 5 at 130,258,993 bp
  • T to A, chromosome 5 at 131,472,450 bp
  • C to T, chromosome 5 at 136,990,011 bp
  • A to C, chromosome 6 at 116,582,697 bp
  • G to T, chromosome 7 at 8,380,123 bp
  • A to T, chromosome 7 at 24,612,694 bp
  • C to A, chromosome 7 at 26,380,490 bp
  • A to C, chromosome 7 at 31,134,575 bp
  • T to A, chromosome 7 at 55,926,395 bp
  • T to A, chromosome 7 at 59,983,459 bp
  • C to T, chromosome 7 at 68,214,933 bp
  • A to G, chromosome 7 at 98,107,095 bp
  • A to G, chromosome 7 at 100,929,870 bp
  • A to G, chromosome 7 at 108,124,915 bp
  • A to G, chromosome 7 at 118,145,798 bp
  • T to C, chromosome 7 at 118,436,838 bp
  • A to G, chromosome 7 at 126,438,799 bp
  • A to G, chromosome 7 at 142,512,364 bp
  • T to C, chromosome 8 at 67,960,565 bp
  • A to G, chromosome 8 at 70,377,146 bp
  • A to G, chromosome 8 at 85,725,512 bp
  • G to A, chromosome 8 at 105,138,097 bp
  • A to G, chromosome 8 at 126,025,384 bp
  • C to G, chromosome 9 at 13,697,345 bp
  • T to C, chromosome 9 at 16,376,985 bp
  • T to C, chromosome 9 at 55,004,651 bp
  • T to A, chromosome 9 at 70,338,784 bp
  • T to C, chromosome 9 at 73,756,339 bp
  • C to A, chromosome 9 at 80,300,572 bp
  • G to A, chromosome 10 at 20,963,115 bp
  • T to C, chromosome 10 at 68,186,067 bp
  • T to C, chromosome 10 at 79,973,408 bp
  • T to C, chromosome 10 at 83,579,525 bp
  • T to C, chromosome 10 at 129,705,607 bp
  • A to T, chromosome 10 at 129,945,582 bp
  • A to G, chromosome 11 at 43,445,242 bp
  • T to A, chromosome 11 at 96,286,781 bp
  • T to G, chromosome 11 at 103,182,079 bp
  • A to T, chromosome 11 at 103,333,005 bp
  • T to A, chromosome 11 at 119,440,221 bp
  • C to A, chromosome 11 at 120,688,560 bp
  • G to T, chromosome 12 at 8,977,435 bp
  • A to G, chromosome 12 at 40,364,085 bp
  • A to T, chromosome 12 at 65,002,635 bp
  • C to A, chromosome 12 at 80,172,954 bp
  • A to T, chromosome 12 at 118,038,780 bp
  • T to C, chromosome 12 at 119,202,455 bp
  • A to G, chromosome 13 at 38,221,360 bp
  • A to G, chromosome 13 at 58,344,008 bp
  • A to G, chromosome 13 at 81,561,583 bp
  • T to C, chromosome 13 at 96,496,632 bp
  • T to A, chromosome 14 at 19,752,431 bp
  • A to G, chromosome 14 at 64,075,730 bp
  • A to G, chromosome 14 at 65,547,919 bp
  • T to A, chromosome 14 at 105,117,073 bp
  • T to G, chromosome 14 at 113,314,947 bp
  • A to T, chromosome 14 at 119,034,942 bp
  • A to G, chromosome 15 at 10,322,536 bp
  • T to G, chromosome 15 at 16,829,120 bp
  • T to G, chromosome 15 at 25,726,525 bp
  • A to G, chromosome 15 at 28,270,426 bp
  • A to T, chromosome 15 at 66,737,548 bp
  • G to A, chromosome 15 at 73,025,967 bp
  • A to T, chromosome 15 at 76,722,578 bp
  • T to A, chromosome 15 at 80,634,331 bp
  • A to T, chromosome 15 at 98,852,074 bp
  • T to G, chromosome 15 at 99,723,202 bp
  • C to A, chromosome 16 at 15,739,524 bp
  • G to A, chromosome 16 at 20,365,951 bp
  • A to T, chromosome 17 at 13,949,584 bp
  • C to A, chromosome 17 at 27,845,219 bp
  • T to A, chromosome 17 at 32,158,725 bp
  • T to C, chromosome 17 at 38,067,948 bp
  • G to A, chromosome 17 at 58,013,921 bp
  • A to G, chromosome 17 at 84,448,033 bp
  • G to T, chromosome 17 at 84,448,034 bp
  • T to A, chromosome 18 at 12,581,680 bp
  • G to A, chromosome 18 at 61,110,510 bp
  • C to G, chromosome 18 at 68,338,670 bp
  • C to T, chromosome 19 at 6,942,057 bp
  • T to C, chromosome 19 at 8,887,029 bp
  • T to C, chromosome 19 at 37,901,818 bp
  • A to G, chromosome 19 at 45,039,228 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1789 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039820-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.