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Strain Name:
C57BL/6J-MtgxR1945Btlr/Mmmh
Stock Number:
039963-MU
Citation ID:
RRID:MMRRC_039963-MU
Other Names:
R1945 (G1), C57BL/6J-MtgxR1945Btlr
Major Collection:

Strain Information

Pitx2
Name: paired-like homeodomain transcription factor 2
Synonyms: Otlx2, Brx1b, Brx1a, Brx1, solurshin, Ptx2, Munc30, Pitx2c, Pitx2b, Pitx2a, Rieg
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18741
HGNC: HGNC:9005
Homologene: 55454
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Atf6
Name: activating transcription factor 6
Synonyms: ESTM49, 9130025P16Rik, Atf6alpha
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226641
HGNC: HGNC:791
Homologene: 32015
Etaa1
Name: Ewing tumor-associated antigen 1
Synonyms: 5730466H23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68145
Homologene: 10369
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 26,682,314 bp
  • C to T, chromosome 1 at 75,430,957 bp
  • A to G, chromosome 1 at 93,271,238 bp
  • T to C, chromosome 1 at 151,696,228 bp
  • A to T, chromosome 1 at 170,855,141 bp
  • C to A, chromosome 1 at 176,793,534 bp
  • T to A, chromosome 2 at 13,466,061 bp
  • T to A, chromosome 2 at 25,609,549 bp
  • A to C, chromosome 2 at 26,375,702 bp
  • T to C, chromosome 2 at 36,453,031 bp
  • A to T, chromosome 2 at 66,675,980 bp
  • T to C, chromosome 2 at 76,730,022 bp
  • T to C, chromosome 2 at 79,662,652 bp
  • T to C, chromosome 2 at 89,296,784 bp
  • T to C, chromosome 2 at 167,617,822 bp
  • T to A, chromosome 3 at 53,505,445 bp
  • A to G, chromosome 3 at 88,383,664 bp
  • A to C, chromosome 3 at 99,939,982 bp
  • G to T, chromosome 3 at 102,777,515 bp
  • A to T, chromosome 3 at 109,107,400 bp
  • A to G, chromosome 3 at 126,834,660 bp
  • A to G, chromosome 3 at 129,218,536 bp
  • T to A, chromosome 3 at 152,379,586 bp
  • A to T, chromosome 4 at 45,965,474 bp
  • A to G, chromosome 4 at 48,614,960 bp
  • ACGTCTTCACCAGGTAATC to AC, chromosome 4 at 53,061,509 bp
  • T to C, chromosome 4 at 116,622,768 bp
  • A to T, chromosome 4 at 123,490,660 bp
  • A to G, chromosome 4 at 135,447,420 bp
  • G to A, chromosome 4 at 138,456,783 bp
  • T to A, chromosome 5 at 135,157,600 bp
  • A to G, chromosome 6 at 15,379,830 bp
  • A to T, chromosome 6 at 48,489,773 bp
  • T to C, chromosome 6 at 58,887,439 bp
  • C to T, chromosome 6 at 102,245,178 bp
  • A to G, chromosome 7 at 7,203,630 bp
  • A to G, chromosome 7 at 18,841,494 bp
  • CGCA to CGCAGCA, chromosome 7 at 27,233,980 bp
  • GCA to GCAACA, chromosome 7 at 27,233,981 bp
  • A to G, chromosome 7 at 49,464,872 bp
  • A to G, chromosome 7 at 139,679,903 bp
  • T to C, chromosome 8 at 13,172,545 bp
  • T to C, chromosome 9 at 42,521,004 bp
  • A to T, chromosome 9 at 67,886,276 bp
  • T to C, chromosome 9 at 102,720,554 bp
  • T to C, chromosome 9 at 107,585,472 bp
  • G to A, chromosome 9 at 108,960,010 bp
  • T to A, chromosome 9 at 109,071,867 bp
  • A to T, chromosome 9 at 119,691,454 bp
  • G to A, chromosome 9 at 121,668,474 bp
  • G to T, chromosome 9 at 121,668,475 bp
  • C to T, chromosome 10 at 59,181,019 bp
  • A to G, chromosome 10 at 67,035,899 bp
  • A to T, chromosome 10 at 107,582,388 bp
  • T to A, chromosome 11 at 6,214,588 bp
  • C to T, chromosome 11 at 6,225,199 bp
  • T to C, chromosome 11 at 9,038,296 bp
  • A to G, chromosome 11 at 17,947,233 bp
  • T to C, chromosome 11 at 32,055,068 bp
  • T to C, chromosome 11 at 46,455,050 bp
  • A to G, chromosome 11 at 54,323,370 bp
  • T to C, chromosome 11 at 59,090,791 bp
  • T to C, chromosome 11 at 60,502,083 bp
  • T to C, chromosome 11 at 61,154,517 bp
  • T to C, chromosome 11 at 68,922,157 bp
  • A to G, chromosome 11 at 74,630,009 bp
  • T to A, chromosome 11 at 87,484,687 bp
  • T to C, chromosome 11 at 97,711,155 bp
  • T to C, chromosome 11 at 100,012,709 bp
  • T to A, chromosome 11 at 100,084,844 bp
  • C to T, chromosome 11 at 100,769,205 bp
  • A to G, chromosome 11 at 106,421,950 bp
  • A to T, chromosome 11 at 115,878,398 bp
  • G to A, chromosome 11 at 115,928,297 bp
  • C to T, chromosome 11 at 115,993,453 bp
  • T to C, chromosome 12 at 80,338,694 bp
  • T to A, chromosome 12 at 85,015,418 bp
  • C to T, chromosome 12 at 111,299,133 bp
  • T to C, chromosome 13 at 33,667,680 bp
  • C to T, chromosome 13 at 50,245,491 bp
  • T to C, chromosome 13 at 64,219,890 bp
  • T to C, chromosome 13 at 73,927,924 bp
  • T to A, chromosome 14 at 49,768,483 bp
  • T to A, chromosome 14 at 51,057,006 bp
  • G to A, chromosome 15 at 76,295,383 bp
  • A to G, chromosome 16 at 4,835,685 bp
  • C to A, chromosome 16 at 35,101,984 bp
  • A to G, chromosome 16 at 56,123,946 bp
  • A to T, chromosome 17 at 34,059,168 bp
  • T to A, chromosome 17 at 55,897,231 bp
  • T to A, chromosome 17 at 67,745,853 bp
  • T to C, chromosome 18 at 5,117,059 bp
  • A to T, chromosome 18 at 37,308,868 bp
  • A to G, chromosome 18 at 77,404,808 bp
  • C to T, chromosome 19 at 11,649,595 bp
  • T to C, chromosome 19 at 28,901,431 bp
  • T to A, chromosome 19 at 38,157,519 bp
  • A to T, chromosome 19 at 41,274,337 bp
  • T to C, chromosome 19 at 44,235,780 bp
  • T to C, chromosome 19 at 48,029,224 bp
  • T to C, chromosome X at 101,441,823 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1945 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039963-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.