Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR1966Btlr/Mmmh
Stock Number:
039979-MU
Citation ID:
RRID:MMRRC_039979-MU
Other Names:
R1966 (G1), C57BL/6J-MtgxR1966Btlr
Major Collection:
Beutler Mutagenetix

Gene Information

Gene Details

Gene Symbol: Tnrc6b [MGI:2443730] (Mus musculus (mouse))
Name: trinucleotide repeat containing 6b
Synonyms: 2700090M07Rik, A730065C02Rik, D230019K20Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Cadps [MGI:1350922] (Mus musculus (mouse))
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Ptx3 [MGI:104641] (Mus musculus (mouse))
Name: pentraxin related gene
Synonyms: TSG-14, pentraxin 3
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Ulk2 [MGI:1352758] (Mus musculus (mouse))
Name: unc-51 like kinase 2
Synonyms: Unc51.2, A830085I22Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Jarid2 [MGI:104813] (Mus musculus (mouse))
Name: jumonji, AT rich interactive domain 2
Synonyms: Jmj, jumonji
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Orc1 [MGI:1328337] (Mus musculus (mouse))
Name: origin recognition complex, subunit 1
Synonyms: Orc1l, MmORC1
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Blm [MGI:1328362] (Mus musculus (mouse))
Name: Bloom syndrome, RecQ like helicase
Chromosome: 7
Alteration at locus: Chemically Induced
See All Alterations
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 51,289,642 bp
  • T to A, chromosome 1 at 88,332,748 bp
  • T to A, chromosome 1 at 134,613,873 bp
  • G to T, chromosome 1 at 174,117,251 bp
  • C to T, chromosome 1 at 186,736,205 bp
  • T to A, chromosome 1 at 187,322,301 bp
  • A to T, chromosome 1 at 194,644,700 bp
  • T to A, chromosome 2 at 28,545,875 bp
  • T to G, chromosome 2 at 31,389,329 bp
  • T to A, chromosome 2 at 34,518,679 bp
  • A to T, chromosome 2 at 35,149,023 bp
  • G to A, chromosome 2 at 36,688,784 bp
  • A to T, chromosome 2 at 37,004,948 bp
  • A to T, chromosome 2 at 55,437,331 bp
  • T to C, chromosome 2 at 82,992,780 bp
  • G to T, chromosome 2 at 84,765,728 bp
  • T to C, chromosome 2 at 90,149,404 bp
  • A to T, chromosome 2 at 127,530,689 bp
  • A to T, chromosome 2 at 150,117,398 bp
  • T to A, chromosome 2 at 150,191,907 bp
  • A to G, chromosome 2 at 153,529,455 bp
  • G to T, chromosome 2 at 164,168,535 bp
  • C to A, chromosome 2 at 180,188,352 bp
  • A to G, chromosome 3 at 28,719,485 bp
  • T to C, chromosome 3 at 33,826,480 bp
  • C to G, chromosome 3 at 52,104,139 bp
  • C to T, chromosome 3 at 66,224,621 bp
  • G to A, chromosome 3 at 79,493,472 bp
  • A to T, chromosome 3 at 79,602,299 bp
  • A to G, chromosome 3 at 86,605,868 bp
  • A to G, chromosome 3 at 89,445,488 bp
  • T to C, chromosome 3 at 107,104,630 bp
  • T to C, chromosome 4 at 43,496,710 bp
  • A to C, chromosome 4 at 53,050,409 bp
  • T to A, chromosome 4 at 108,612,217 bp
  • A to G, chromosome 4 at 115,625,879 bp
  • A to G, chromosome 4 at 139,451,244 bp
  • T to C, chromosome 4 at 150,616,873 bp
  • C to T, chromosome 5 at 16,333,785 bp
  • T to A, chromosome 5 at 115,294,831 bp
  • T to A, chromosome 5 at 121,439,820 bp
  • A to G, chromosome 6 at 29,418,092 bp
  • A to T, chromosome 6 at 132,711,194 bp
  • C to A, chromosome 6 at 138,658,445 bp
  • A to T, chromosome 7 at 3,717,638 bp
  • T to A, chromosome 7 at 7,284,185 bp
  • T to A, chromosome 7 at 11,930,514 bp
  • T to C, chromosome 7 at 16,079,071 bp
  • T to C, chromosome 7 at 26,611,531 bp
  • C to A, chromosome 7 at 29,792,452 bp
  • T to A, chromosome 7 at 44,251,226 bp
  • A to G, chromosome 7 at 47,310,219 bp
  • A to G, chromosome 7 at 56,414,467 bp
  • T to C, chromosome 7 at 78,843,218 bp
  • C to A, chromosome 7 at 79,694,735 bp
  • A to T, chromosome 7 at 80,513,186 bp
  • G to T, chromosome 7 at 103,755,168 bp
  • A to G, chromosome 7 at 104,596,801 bp
  • A to G, chromosome 7 at 128,755,353 bp
  • A to G, chromosome 7 at 130,753,671 bp
  • A to G, chromosome 7 at 141,803,376 bp
  • C to A, chromosome 8 at 22,545,537 bp
  • T to G, chromosome 8 at 61,016,296 bp
  • A to T, chromosome 8 at 84,676,381 bp
  • C to A, chromosome 8 at 93,944,199 bp
  • T to G, chromosome 8 at 111,450,903 bp
  • G to A, chromosome 9 at 13,828,066 bp
  • T to C, chromosome 9 at 21,833,361 bp
  • C to A, chromosome 9 at 49,068,833 bp
  • A to T, chromosome 9 at 110,476,590 bp
  • A to G, chromosome 9 at 114,914,394 bp
  • T to A, chromosome 9 at 121,257,116 bp
  • A to T, chromosome 10 at 24,807,491 bp
  • G to A, chromosome 10 at 25,441,768 bp
  • A to T, chromosome 10 at 49,355,909 bp
  • T to C, chromosome 10 at 60,323,582 bp
  • T to C, chromosome 11 at 59,373,484 bp
  • A to T, chromosome 11 at 61,819,471 bp
  • G to T, chromosome 11 at 68,954,675 bp
  • T to A, chromosome 11 at 79,411,564 bp
  • C to T, chromosome 11 at 82,901,788 bp
  • T to C, chromosome 11 at 83,346,895 bp
  • A to G, chromosome 11 at 88,952,414 bp
  • T to A, chromosome 11 at 98,204,090 bp
  • T to C, chromosome 11 at 101,180,386 bp
  • A to T, chromosome 13 at 16,026,636 bp
  • T to A, chromosome 13 at 44,906,276 bp
  • A to G, chromosome 13 at 49,039,011 bp
  • A to T, chromosome 13 at 55,014,455 bp
  • C to A, chromosome 14 at 12,822,450 bp
  • T to A, chromosome 14 at 51,071,157 bp
  • T to A, chromosome 14 at 122,927,599 bp
  • C to T, chromosome 15 at 27,889,531 bp
  • A to T, chromosome 15 at 63,892,616 bp
  • A to G, chromosome 15 at 80,880,439 bp
  • T to G, chromosome 15 at 89,164,198 bp
  • T to C, chromosome 15 at 101,848,992 bp
  • A to G, chromosome 16 at 8,830,581 bp
  • T to C, chromosome 16 at 19,982,822 bp
  • A to T, chromosome 16 at 32,233,230 bp
  • A to G, chromosome 16 at 32,914,385 bp
  • A to T, chromosome 16 at 33,814,539 bp
  • T to C, chromosome 17 at 6,303,226 bp
  • T to C, chromosome 17 at 14,934,599 bp
  • T to C, chromosome 17 at 40,308,846 bp
  • T to C, chromosome 17 at 46,954,919 bp
  • T to A, chromosome 17 at 56,112,186 bp
  • A to G, chromosome 17 at 87,678,369 bp
  • C to T, chromosome 18 at 21,554,673 bp
  • C to A, chromosome 19 at 8,218,408 bp
  • C to T, chromosome 19 at 36,089,149 bp
  • T to C, chromosome 19 at 46,271,564 bp
  • T to G, chromosome 19 at 58,975,038 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1966 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039979-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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