Strain Name:
C57BL/6J-MtgxR1970Btlr/Mmmh
Stock Number:
039983-MU
Citation ID:
RRID:MMRRC_039983-MU
Other Names:
R1970 (G1), C57BL/6J-MtgxR1970Btlr
Major Collection:

Gene Information

Gene Symbol: Gnb5 [MGI:101848] (Mus musculus (mouse))
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: G beta 5, Gbeta5
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Foxp4 [MGI:1921373] (Mus musculus (mouse))
Name: forkhead box P4
Synonyms: 1200010K03Rik, 2310007G05Rik
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Vcan [MGI:102889] (Mus musculus (mouse))
Name: versican
Synonyms: 5430420N07Rik, hdf, PG-M, DPEAAE, Cspg2, heart defect
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Syt6 [MGI:1859544] (Mus musculus (mouse))
Name: synaptotagmin VI
Synonyms: 3110037A08Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Sez6 [MGI:104745] (Mus musculus (mouse))
Name: seizure related gene 6
Synonyms: sez-6, D11Bhm177e
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Snx2 [MGI:1915054] (Mus musculus (mouse))
Name: sorting nexin 2
Synonyms: 0610030A03Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Ddc [MGI:94876] (Mus musculus (mouse))
Name: dopa decarboxylase
Synonyms: aromatic L-amino acid decarboxylase, Aadc
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,381,523 bp
  • T to C, chromosome 1 at 36,151,781 bp
  • T to C, chromosome 1 at 42,230,721 bp
  • A to T, chromosome 1 at 59,215,169 bp
  • T to A, chromosome 1 at 74,285,910 bp
  • C to T, chromosome 1 at 135,372,274 bp
  • T to C, chromosome 1 at 136,171,156 bp
  • T to G, chromosome 1 at 156,366,447 bp
  • T to G, chromosome 1 at 165,306,358 bp
  • T to C, chromosome 1 at 174,240,367 bp
  • A to T, chromosome 2 at 27,986,754 bp
  • T to C, chromosome 2 at 29,733,711 bp
  • T to C, chromosome 2 at 40,875,069 bp
  • A to T, chromosome 2 at 52,263,905 bp
  • T to A, chromosome 2 at 53,105,576 bp
  • G to T, chromosome 2 at 66,515,380 bp
  • A to G, chromosome 2 at 66,684,289 bp
  • C to T, chromosome 2 at 69,482,874 bp
  • T to C, chromosome 2 at 69,833,686 bp
  • C to T, chromosome 2 at 80,517,942 bp
  • T to A, chromosome 2 at 86,228,252 bp
  • T to C, chromosome 2 at 90,889,659 bp
  • C to T, chromosome 2 at 111,369,076 bp
  • T to C, chromosome 2 at 125,747,510 bp
  • T to C, chromosome 2 at 127,246,996 bp
  • G to A, chromosome 2 at 162,959,572 bp
  • A to T, chromosome 3 at 32,598,141 bp
  • A to T, chromosome 3 at 84,454,851 bp
  • T to C, chromosome 3 at 88,024,120 bp
  • T to C, chromosome 3 at 88,327,870 bp
  • C to T, chromosome 3 at 88,376,993 bp
  • C to T, chromosome 3 at 95,448,393 bp
  • A to G, chromosome 3 at 103,587,420 bp
  • C to A, chromosome 3 at 159,615,670 bp
  • C to T, chromosome 4 at 63,273,117 bp
  • G to T, chromosome 4 at 107,968,585 bp
  • T to A, chromosome 4 at 116,163,629 bp
  • A to G, chromosome 4 at 129,226,017 bp
  • T to C, chromosome 5 at 9,148,989 bp
  • A to G, chromosome 5 at 14,713,473 bp
  • G to T, chromosome 5 at 30,117,174 bp
  • A to G, chromosome 5 at 106,973,074 bp
  • A to C, chromosome 5 at 111,235,635 bp
  • T to C, chromosome 5 at 125,038,918 bp
  • T to A, chromosome 5 at 128,797,241 bp
  • T to C, chromosome 6 at 115,962,517 bp
  • T to C, chromosome 6 at 124,414,405 bp
  • T to A, chromosome 6 at 139,900,386 bp
  • A to T, chromosome 7 at 5,454,039 bp
  • T to C, chromosome 7 at 23,834,948 bp
  • T to A, chromosome 7 at 25,229,721 bp
  • A to G, chromosome 7 at 46,869,751 bp
  • A to T, chromosome 7 at 85,151,805 bp
  • A to T, chromosome 7 at 86,148,262 bp
  • T to A, chromosome 7 at 90,244,154 bp
  • C to T, chromosome 7 at 102,009,322 bp
  • T to A, chromosome 7 at 106,677,670 bp
  • G to A, chromosome 7 at 106,914,246 bp
  • A to T, chromosome 7 at 120,780,171 bp
  • G to T, chromosome 7 at 127,444,787 bp
  • G to A, chromosome 7 at 141,212,059 bp
  • A to G, chromosome 8 at 36,129,160 bp
  • A to T, chromosome 8 at 61,972,206 bp
  • A to T, chromosome 8 at 68,896,802 bp
  • C to T, chromosome 8 at 70,176,909 bp
  • A to G, chromosome 8 at 86,527,281 bp
  • T to C, chromosome 8 at 105,241,148 bp
  • A to G, chromosome 8 at 105,786,708 bp
  • T to C, chromosome 9 at 21,941,126 bp
  • T to C, chromosome 9 at 35,213,538 bp
  • T to A, chromosome 9 at 39,873,938 bp
  • A to G, chromosome 9 at 65,795,277 bp
  • T to C, chromosome 9 at 70,368,773 bp
  • T to A, chromosome 9 at 71,725,535 bp
  • A to G, chromosome 9 at 75,344,650 bp
  • A to G, chromosome 9 at 83,780,719 bp
  • A to T, chromosome 9 at 96,576,272 bp
  • A to G, chromosome 10 at 42,197,262 bp
  • G to A, chromosome 10 at 78,441,154 bp
  • A to G, chromosome 11 at 11,815,292 bp
  • A to G, chromosome 11 at 21,328,942 bp
  • A to G, chromosome 11 at 35,630,841 bp
  • A to G, chromosome 11 at 59,069,726 bp
  • A to C, chromosome 11 at 70,646,173 bp
  • G to A, chromosome 11 at 77,954,068 bp
  • A to G, chromosome 11 at 79,553,961 bp
  • T to A, chromosome 11 at 116,151,491 bp
  • A to G, chromosome 12 at 52,542,593 bp
  • T to A, chromosome 12 at 52,938,475 bp
  • G to A, chromosome 12 at 80,758,712 bp
  • T to A, chromosome 12 at 83,325,035 bp
  • A to T, chromosome 12 at 104,103,857 bp
  • T to C, chromosome 12 at 116,136,206 bp
  • A to C, chromosome 13 at 19,652,322 bp
  • A to G, chromosome 13 at 21,983,472 bp
  • T to C, chromosome 13 at 65,194,091 bp
  • A to T, chromosome 13 at 89,689,038 bp
  • T to A, chromosome 13 at 92,249,820 bp
  • C to T, chromosome 13 at 112,600,013 bp
  • A to G, chromosome 14 at 41,061,903 bp
  • T to C, chromosome 14 at 55,624,142 bp
  • C to G, chromosome 14 at 57,801,907 bp
  • G to T, chromosome 14 at 60,691,463 bp
  • C to G, chromosome 14 at 68,086,672 bp
  • T to C, chromosome 14 at 75,200,506 bp
  • T to C, chromosome 15 at 48,673,531 bp
  • T to C, chromosome 15 at 51,967,775 bp
  • T to C, chromosome 15 at 54,882,982 bp
  • A to G, chromosome 15 at 66,028,623 bp
  • T to C, chromosome 15 at 74,539,877 bp
  • G to T, chromosome 15 at 76,006,570 bp
  • T to C, chromosome 16 at 4,633,756 bp
  • T to C, chromosome 16 at 13,789,074 bp
  • A to G, chromosome 16 at 16,883,519 bp
  • A to G, chromosome 16 at 31,133,527 bp
  • A to G, chromosome 16 at 33,977,524 bp
  • A to T, chromosome 17 at 24,307,575 bp
  • A to T, chromosome 17 at 33,335,501 bp
  • A to G, chromosome 17 at 35,033,600 bp
  • C to T, chromosome 17 at 47,875,871 bp
  • G to T, chromosome 17 at 56,073,077 bp
  • G to T, chromosome 17 at 84,310,042 bp
  • AATCATTTG to AG, chromosome 17 at 84,673,602 bp
  • T to A, chromosome 18 at 53,189,644 bp
  • G to A, chromosome 18 at 61,066,494 bp
  • C to A, chromosome 18 at 67,229,772 bp
  • G to T, chromosome 19 at 9,885,487 bp
  • A to T, chromosome 19 at 37,945,634 bp
  • T to C, chromosome 19 at 40,940,165 bp
  • T to C, chromosome X at 166,427,214 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1970 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039983-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.