Strain Name:
C57BL/6J-MtgxR2147Btlr/Mmmh
Stock Number:
040150-MU
Citation ID:
RRID:MMRRC_040150-MU
Other Names:
R2147 (G1), C57BL/6J-MtgxR2147Btlr
Major Collection:

Gene Information

Gene Symbol: Vps41 [MGI:1929215] (Mus musculus (mouse))
Name: VPS41 HOPS complex subunit
Synonyms: mVam2, Vam2
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Lamb3 [MGI:99915] (Mus musculus (mouse))
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Pitpnc1 [MGI:1919045] (Mus musculus (mouse))
Name: phosphatidylinositol transfer protein, cytoplasmic 1
Synonyms: 1110020B03Rik, C330017I21Rik, 5830436L09Rik, RDGBB1, RDGB-BETA
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Mbtps1 [MGI:1927235] (Mus musculus (mouse))
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: SKI-1, S1P, 0610038M03Rik, subtilisin/kexin isozyme-1, site-1 protease
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Adam23 [MGI:1345162] (Mus musculus (mouse))
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Lrsam1 [MGI:2684789] (Mus musculus (mouse))
Name: leucine rich repeat and sterile alpha motif containing 1
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Mycbp2 [MGI:2179432] (Mus musculus (mouse))
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Chromosome: 14
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 63,534,362 bp
  • C to T, chromosome 1 at 82,470,978 bp
  • T to A, chromosome 1 at 160,075,433 bp
  • T to C, chromosome 1 at 160,195,292 bp
  • T to C, chromosome 1 at 193,327,904 bp
  • A to G, chromosome 2 at 14,219,050 bp
  • T to C, chromosome 2 at 32,945,879 bp
  • T to A, chromosome 2 at 94,301,794 bp
  • T to G, chromosome 2 at 120,864,330 bp
  • A to G, chromosome 2 at 125,087,441 bp
  • T to A, chromosome 4 at 19,536,562 bp
  • T to C, chromosome 4 at 19,608,952 bp
  • T to A, chromosome 4 at 24,580,063 bp
  • C to A, chromosome 4 at 53,491,379 bp
  • C to G, chromosome 4 at 111,937,077 bp
  • A to G, chromosome 4 at 129,118,485 bp
  • A to T, chromosome 4 at 133,681,366 bp
  • A to G, chromosome 4 at 155,966,465 bp
  • T to C, chromosome 4 at 156,334,719 bp
  • T to A, chromosome 5 at 24,592,035 bp
  • T to C, chromosome 5 at 54,105,375 bp
  • T to A, chromosome 5 at 58,129,116 bp
  • T to A, chromosome 5 at 65,809,200 bp
  • T to C, chromosome 5 at 87,001,618 bp
  • T to C, chromosome 5 at 123,256,191 bp
  • G to T, chromosome 6 at 23,838,999 bp
  • T to C, chromosome 6 at 30,118,939 bp
  • A to T, chromosome 6 at 40,207,501 bp
  • A to G, chromosome 6 at 57,137,598 bp
  • A to G, chromosome 6 at 92,425,671 bp
  • A to G, chromosome 6 at 92,887,818 bp
  • T to C, chromosome 6 at 103,715,401 bp
  • T to C, chromosome 6 at 128,890,904 bp
  • T to A, chromosome 6 at 142,771,289 bp
  • G to T, chromosome 7 at 25,388,521 bp
  • A to T, chromosome 7 at 66,285,411 bp
  • A to T, chromosome 7 at 81,126,267 bp
  • T to A, chromosome 7 at 125,865,320 bp
  • T to C, chromosome 7 at 127,610,444 bp
  • A to G, chromosome 7 at 141,892,170 bp
  • G to A, chromosome 8 at 22,065,293 bp
  • A to G, chromosome 8 at 43,652,501 bp
  • A to T, chromosome 8 at 119,538,859 bp
  • A to G, chromosome 9 at 31,428,981 bp
  • A to T, chromosome 9 at 110,622,175 bp
  • A to G, chromosome 10 at 41,740,899 bp
  • C to A, chromosome 10 at 100,396,552 bp
  • A to G, chromosome 11 at 34,229,472 bp
  • A to G, chromosome 11 at 60,510,229 bp
  • A to T, chromosome 11 at 69,349,028 bp
  • T to C, chromosome 11 at 69,515,761 bp
  • A to T, chromosome 11 at 70,319,945 bp
  • A to G, chromosome 11 at 75,490,531 bp
  • T to C, chromosome 11 at 76,455,648 bp
  • T to A, chromosome 11 at 78,521,244 bp
  • T to C, chromosome 11 at 84,079,629 bp
  • T to G, chromosome 11 at 84,276,536 bp
  • C to T, chromosome 11 at 107,212,518 bp
  • T to A, chromosome 12 at 40,080,985 bp
  • T to A, chromosome 12 at 104,389,224 bp
  • T to A, chromosome 12 at 104,953,098 bp
  • T to C, chromosome 12 at 111,446,022 bp
  • G to T, chromosome 13 at 18,839,734 bp
  • C to T, chromosome 13 at 34,183,007 bp
  • T to C, chromosome 13 at 48,804,689 bp
  • G to A, chromosome 13 at 52,835,496 bp
  • T to C, chromosome 13 at 67,041,896 bp
  • A to G, chromosome 14 at 28,513,317 bp
  • T to C, chromosome 14 at 49,751,571 bp
  • A to G, chromosome 14 at 59,597,753 bp
  • G to A, chromosome 14 at 103,155,922 bp
  • CCTTTGCGCTT to CCTT, chromosome 15 at 47,741,236 bp
  • T to G, chromosome 15 at 73,523,487 bp
  • T to C, chromosome 15 at 89,503,926 bp
  • T to C, chromosome 15 at 103,455,938 bp
  • A to G, chromosome 16 at 44,451,684 bp
  • T to A, chromosome 16 at 58,824,479 bp
  • T to C, chromosome 17 at 7,334,302 bp
  • A to T, chromosome 17 at 24,230,314 bp
  • T to A, chromosome 17 at 24,621,142 bp
  • A to G, chromosome 17 at 26,636,050 bp
  • T to C, chromosome 17 at 31,098,238 bp
  • T to A, chromosome 17 at 71,398,588 bp
  • A to T, chromosome 19 at 5,667,605 bp
  • T to C, chromosome 19 at 34,827,354 bp
  • A to T, chromosome 19 at 46,329,188 bp
  • A to G, chromosome 19 at 47,104,752 bp
  • G to A, chromosome X at 12,057,623 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2147 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040150-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.