Beutler Mutagenetix

Strain Name:
C57BL/6J-MtgxR2162Btlr/Mmmh
Stock Number:
040165-MU
Citation ID:
RRID:MMRRC_040165-MU
Other Names:
R2162 (G1), C57BL/6J-MtgxR2162Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223838
Homologene: 11808
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20564
Homologene: 2303
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna2, Spna-2, 2610027H02Rik, alphaII-spectrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20740
Homologene: 2353
Bcat1
Name: branched chain aminotransferase 1, cytosolic
Synonyms: Bcat-1, Eca39, BCATc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 12035
HGNC: HGNC:976
Homologene: 20320
Zcchc17
Name: zinc finger, CCHC domain containing 17
Synonyms: HSPC251, Ps1d, 2810055E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 619605
Homologene: 32319
Mark2
Name: MAP/microtubule affinity regulating kinase 2
Synonyms: Emk, Par-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 13728
HGNC: HGNC:3332
Homologene: 69013
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: GCN1L, G431004K08Rik, Gcn1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Pan2
Name: PAN2 poly(A) specific ribonuclease subunit
Synonyms: 1200014O24Rik, Usp52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 103135
VEGA: 10
Homologene: 5918
Zfp280d
Name: zinc finger protein 280D
Synonyms: Suhw4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235469
Homologene: 17151
Zfp516
Name: zinc finger protein 516
Synonyms: Zfp26l, C330029B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 329003
VEGA: 18
Homologene: 37122
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, S-afadin, Mllt4, I-afadin, 5033403D15Rik, Afadin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Tdrd3
Name: tudor domain containing 3
Synonyms: 4732418C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 219249
Homologene: 12771
S100a10
Name: S100 calcium binding protein A10 (calpactin)
Synonyms: p10, 42C, CLP11, CAL12, Cal1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 20194
Homologene: 2228
Cyfip2
Name: cytoplasmic FMR1 interacting protein 2
Synonyms: Pir121, 6430511D02Rik, 1500004I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76884
Homologene: 7936
Ankrd28
Name: ankyrin repeat domain 28
Synonyms: E430019N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105522
VEGA: 14
Homologene: 35374
Ttll4
Name: tubulin tyrosine ligase-like family, member 4
Synonyms: 4632407P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 67534
Homologene: 8764
Dcc
Name: deleted in colorectal carcinoma
Synonyms: Igdcc1, C030036D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 13176
HGNC: HGNC:2701
Homologene: 21081
Dnaaf5
Name: dynein, axonemal assembly factor 5
Synonyms: Heatr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 433956
Homologene: 41198
Pdp1
Name: pyruvate dehyrogenase phosphatase catalytic subunit 1
Synonyms: Ppm2c, LOC381511
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 381511
HGNC: HGNC:9279
Homologene: 31928
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Il1f5
Name: interleukin 1 family, member 5 (delta)
Synonyms: IL-1H3, FIL1delta, IL1F5, IL1HY1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 54450
Homologene: 10453
Eng
Name: endoglin
Synonyms: Endo, CD105
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 13805
HGNC: HGNC:3349
Homologene: 92
Htt
Name: huntingtin
Synonyms: huntingtin, IT15, HD, C430023I11Rik, htt, Hdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Lzic
Name: leucine zipper and CTNNBIP1 domain containing
Synonyms: 1810030J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69151
Homologene: 12301
Zfp445
Name: zinc finger protein 445
Synonyms: ZNF168
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235682
VEGA: 9
Homologene: 27832
Pja2
Name: praja ring finger ubiquitin ligase 2
Synonyms: Neurodap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224938
Homologene: 32233
Dpp9
Name: dipeptidylpeptidase 9
Synonyms: DPRP2, 6430584G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224897
VEGA: 17
Homologene: 16385
Peg10
Name: paternally expressed 10
Synonyms: HB-1, MEF3L, Mar2, Rtl2, MyEF-3 like, Edr, MyEF-3, Mart2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 170676
Homologene: 116067
Atp6v0a4
Name: ATPase, H+ transporting, lysosomal V0 subunit A4
Synonyms: V-ATPase alpha 4, Atp6n1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 140494
HGNC: HGNC:866
Homologene: 39904
Mroh7
Name: maestro heat-like repeat family member 7
Synonyms: Gm1027, LOC381538, Heatr8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 381538
Homologene: 19633
Mep1b
Name: meprin 1 beta
Synonyms: Mep-1b, meprin beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 17288
VEGA: 18
HGNC: HGNC:7020
Homologene: 48382
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Dnahc12, HL-19, DLP12, HL19, DHC3, 4921531P07Rik, Hdhc3, Dnahc7l, LOC380889
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Kcnn3
Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3
Synonyms: small conductance calcium-activated potassium channel 3, SK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 140493
HGNC: HGNC:6292
Homologene: 20516
Acsl1
Name: acyl-CoA synthetase long-chain family member 1
Synonyms: Acas1, Facl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 14081
HGNC: HGNC:3569
Homologene: 37561
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242627
HGNC: null
Homologene: 135888
1700022I11Rik
Name: RIKEN cDNA 1700022I11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 67317
Homologene: 52406
Scn9a
Name: sodium channel, voltage-gated, type IX, alpha
Synonyms: PN1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20274
Homologene: 2237
Gprc6a
Name: G protein-coupled receptor, family C, group 6, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 210198
VEGA: 10
Homologene: 17529
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239420
Homologene: 65982
Vmn1r167
Name: vomeronasal 1 receptor 167
Synonyms: Gm6279
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 622032
HGNC: null
Homologene: 128341
Tbc1d9
Name: TBC1 domain family, member 9
Synonyms: C76116, 4933431N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71310
Homologene: 57079
Cacna1i
Name: calcium channel, voltage-dependent, alpha 1I subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239556
HGNC: HGNC:1396
Homologene: 69331
Ppp1r9b
Name: protein phosphatase 1, regulatory subunit 9B
Synonyms: spinophilin, SPL, neurabin II, Spn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217124
HGNC: HGNC:9298
Homologene: 32787
Fam38b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
Itga1
Name: integrin alpha 1
Synonyms: CD49A, E130012M19Rik, Vla1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 109700
VEGA: 13
HGNC: HGNC:6134
Homologene: 57137
Nrxn1
Name: neurexin I
Synonyms: neurexin I beta, neurexin I beta, neurexin I alpha, neurexin I alpha, A230068P09Rik, 1700062G21Rik, neurexin I alpha, neurexin I beta, alpha-latrotoxin receptor (calcium-dependent), 9330127H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 18189
HGNC: HGNC:8008
Homologene: 21005
Olfr494
Name: olfactory receptor 494
Synonyms: GA_x6K02T2PBJ9-10697517-10698461, MOR204-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258732
HGNC: null
Homologene: 133602
Tectb
Name: tectorin beta
Synonyms: [b]-tectorin, Tctnb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 21684
Homologene: 7568
Whamm
Name: WAS protein homolog associated with actin, golgi membranes and microtubules
Synonyms: Whdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 434204
Homologene: 45851
Usp43
Name: ubiquitin specific peptidase 43
Synonyms: C630032K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216835
Homologene: 129879
Pdzd7
Name: PDZ domain containing 7
Synonyms: Pdzk7, EG435601
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 100503041
Homologene: 129509
Pacs2
Name: phosphofurin acidic cluster sorting protein 2
Synonyms: Pacs1l, 6720425G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217893
VEGA: 12
Homologene: 15016
Srrd
Name: SRR1 domain containing
Synonyms: Srr1, 2810002G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 70118
Homologene: 33447
Olfr624
Name: olfactory receptor 624
Synonyms: GA_x6K02T2PBJ9-6394126-6393197, MOR4-2P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258189
HGNC: null
Homologene: 66460
BC048403
Name: cDNA sequence BC048403
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 270802
Homologene: 17580
Cyp27b1
Name: cytochrome P450, family 27, subfamily b, polypeptide 1
Synonyms: 1alpha(OH)ase, 25-hydroxyvitamin D3 1alpha-hydroxylase, 25(OH)D 1alpha-hydroxylase, VddrI, P450c1, Cyp27b, Vddr, Vdd1, Pddr, Cyp1, Cp2b, Cyp40, P450VD1alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 13115
HGNC: HGNC:2606
Homologene: 37139
Hlx
Name: H2.0-like homeobox
Synonyms: Hlx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 15284
HGNC: HGNC:4978
Homologene: 7363
Arhgap45
Name: Rho GTPase activating protein 45
Synonyms: Hmha1, 6330406L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 70719
Homologene: 69120
Pgc
Name: progastricsin (pepsinogen C)
Synonyms: Upg-1, 2210410L06Rik, Upg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 109820
HGNC: HGNC:8890
Homologene: 55669
Ptgir
Name: prostaglandin I receptor (IP)
Synonyms: prostacyclin receptor, IP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 19222
HGNC: HGNC:9602
Homologene: 7496
Olfr1145
Name: olfactory receptor 1145
Synonyms: MOR264-19, GA_x6K02T2Q125-49311440-49312384
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258317
HGNC: null
Homologene: 27123
Krtap19-3
Name: keratin associated protein 19-3
Synonyms: Krtap16-5, A030006N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 77918
VEGA: 16
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,686,391 bp
  • T to A, chromosome 1 at 184,730,692 bp
  • G to T, chromosome 2 at 24,279,680 bp
  • T to C, chromosome 2 at 30,018,576 bp
  • C to T, chromosome 2 at 32,679,047 bp
  • T to A, chromosome 2 at 66,534,229 bp
  • T to A, chromosome 2 at 87,810,360 bp
  • T to C, chromosome 3 at 89,667,077 bp
  • T to C, chromosome 3 at 93,564,373 bp
  • T to C, chromosome 3 at 144,928,587 bp
  • A to G, chromosome 4 at 11,961,123 bp
  • A to G, chromosome 4 at 42,972,238 bp
  • G to C, chromosome 4 at 106,700,181 bp
  • A to T, chromosome 4 at 113,885,831 bp
  • T to C, chromosome 4 at 130,338,524 bp
  • G to C, chromosome 4 at 149,488,728 bp
  • T to A, chromosome 5 at 34,821,718 bp
  • G to T, chromosome 5 at 112,342,944 bp
  • A to T, chromosome 5 at 115,592,132 bp
  • T to C, chromosome 5 at 139,181,565 bp
  • T to A, chromosome 6 at 4,755,914 bp
  • T to G, chromosome 6 at 38,088,646 bp
  • T to A, chromosome 6 at 145,010,108 bp
  • A to G, chromosome 7 at 16,906,869 bp
  • T to C, chromosome 7 at 23,504,799 bp
  • A to G, chromosome 7 at 81,571,341 bp
  • T to G, chromosome 7 at 103,670,872 bp
  • C to A, chromosome 7 at 108,367,562 bp
  • T to G, chromosome 8 at 46,508,320 bp
  • T to G, chromosome 8 at 83,260,904 bp
  • T to A, chromosome 9 at 72,298,822 bp
  • T to G, chromosome 9 at 122,852,476 bp
  • CAAA to CA, chromosome 10 at 51,615,680 bp
  • T to C, chromosome 10 at 80,016,979 bp
  • T to C, chromosome 10 at 121,745,316 bp
  • G to T, chromosome 10 at 127,051,060 bp
  • A to G, chromosome 10 at 128,304,222 bp
  • A to T, chromosome 11 at 35,688,682 bp
  • T to C, chromosome 11 at 46,261,506 bp
  • A to G, chromosome 11 at 67,879,969 bp
  • T to C, chromosome 11 at 94,998,051 bp
  • A to G, chromosome 12 at 113,050,947 bp
  • C to T, chromosome 13 at 115,030,910 bp
  • T to C, chromosome 14 at 26,872,805 bp
  • T to C, chromosome 14 at 31,708,762 bp
  • A to G, chromosome 14 at 87,480,785 bp
  • CCTTTGCGCTT to CCTT, chromosome 15 at 47,741,236 bp
  • T to C, chromosome 15 at 80,356,187 bp
  • A to C, chromosome 15 at 94,331,458 bp
  • T to G, chromosome 16 at 88,877,719 bp
  • T to A, chromosome 17 at 13,896,174 bp
  • C to A, chromosome 17 at 47,729,311 bp
  • A to T, chromosome 17 at 56,199,113 bp
  • T to C, chromosome 17 at 64,309,402 bp
  • G to A, chromosome 17 at 90,162,431 bp
  • C to T, chromosome 18 at 21,086,239 bp
  • A to G, chromosome 18 at 63,081,662 bp
  • C to A, chromosome 18 at 71,682,326 bp
  • A to G, chromosome 18 at 82,986,938 bp
  • G to C, chromosome 19 at 7,282,747 bp
  • A to G, chromosome 19 at 45,036,055 bp
  • C to G, chromosome 19 at 55,180,999 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2162 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040165-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.