Strain Name:
C57BL/6J-MtgxR2291Btlr/Mmmh
Stock Number:
040290-MU
Citation ID:
RRID:MMRRC_040290-MU
Other Names:
R2291 (G1), C57BL/6J-MtgxR2291Btlr
Major Collection:

Gene Information

Gene Symbol: Camk2a [MGI:88256] (Mus musculus (mouse))
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Pax6 [MGI:97490] (Mus musculus (mouse))
Name: paired box 6
Synonyms: Pax-6, 1500038E17Rik, Dey, Dickie's small eye, AEY11, Gsfaey11
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Otx1 [MGI:97450] (Mus musculus (mouse))
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Clasp1 [MGI:1923957] (Mus musculus (mouse))
Name: CLIP associating protein 1
Synonyms: mCLASP1, 5730583A19Rik, CLASP1, 1700030C23Rik, B130045P17Rik, CLASP1alpha
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Atm [MGI:107202] (Mus musculus (mouse))
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Plcb4 [MGI:107464] (Mus musculus (mouse))
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Wnt7a [MGI:98961] (Mus musculus (mouse))
Name: wingless-type MMTV integration site family, member 7A
Synonyms: Wnt-7a, tw
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 74,926,475 bp
  • T to A, chromosome 1 at 118,499,635 bp
  • A to G, chromosome 1 at 125,677,541 bp
  • T to A, chromosome 1 at 146,901,074 bp
  • C to A, chromosome 1 at 149,901,189 bp
  • C to T, chromosome 1 at 152,536,281 bp
  • T to C, chromosome 1 at 154,403,683 bp
  • T to C, chromosome 1 at 172,244,906 bp
  • T to G, chromosome 1 at 191,606,203 bp
  • A to G, chromosome 2 at 52,325,081 bp
  • A to G, chromosome 2 at 66,288,968 bp
  • G to A, chromosome 2 at 85,042,316 bp
  • A to T, chromosome 2 at 86,315,180 bp
  • A to C, chromosome 2 at 104,660,507 bp
  • A to C, chromosome 2 at 105,685,883 bp
  • A to T, chromosome 2 at 120,877,070 bp
  • A to T, chromosome 2 at 135,939,983 bp
  • T to C, chromosome 3 at 97,591,283 bp
  • T to C, chromosome 3 at 103,761,610 bp
  • A to T, chromosome 3 at 124,413,801 bp
  • T to A, chromosome 4 at 12,974,817 bp
  • T to A, chromosome 4 at 62,150,185 bp
  • A to G, chromosome 4 at 118,449,000 bp
  • T to A, chromosome 4 at 130,067,040 bp
  • T to C, chromosome 4 at 136,933,347 bp
  • A to T, chromosome 4 at 136,985,017 bp
  • A to T, chromosome 5 at 21,001,267 bp
  • C to A, chromosome 5 at 32,979,402 bp
  • T to G, chromosome 5 at 108,332,917 bp
  • G to T, chromosome 6 at 53,818,027 bp
  • T to C, chromosome 6 at 57,258,692 bp
  • G to T, chromosome 6 at 83,037,488 bp
  • C to T, chromosome 6 at 91,394,486 bp
  • T to A, chromosome 6 at 103,715,393 bp
  • C to T, chromosome 7 at 29,098,777 bp
  • C to T, chromosome 7 at 81,591,771 bp
  • T to C, chromosome 7 at 86,920,154 bp
  • A to T, chromosome 7 at 105,091,103 bp
  • G to T, chromosome 7 at 105,091,104 bp
  • C to T, chromosome 7 at 109,038,883 bp
  • T to C, chromosome 8 at 39,624,222 bp
  • T to C, chromosome 8 at 95,323,750 bp
  • T to A, chromosome 8 at 105,336,964 bp
  • T to C, chromosome 9 at 39,950,334 bp
  • C to T, chromosome 9 at 53,490,909 bp
  • T to C, chromosome 9 at 56,892,743 bp
  • C to T, chromosome 10 at 81,467,047 bp
  • T to C, chromosome 10 at 93,503,536 bp
  • T to C, chromosome 10 at 127,048,294 bp
  • C to A, chromosome 10 at 128,207,610 bp
  • T to A, chromosome 11 at 21,996,634 bp
  • T to A, chromosome 11 at 34,819,309 bp
  • T to C, chromosome 11 at 51,646,103 bp
  • C to T, chromosome 11 at 60,851,738 bp
  • T to C, chromosome 11 at 62,283,693 bp
  • A to G, chromosome 11 at 69,603,567 bp
  • T to C, chromosome 11 at 70,649,303 bp
  • G to A, chromosome 11 at 86,733,622 bp
  • G to A, chromosome 11 at 99,145,335 bp
  • G to T, chromosome 11 at 102,908,270 bp
  • A to G, chromosome 11 at 114,790,193 bp
  • A to T, chromosome 11 at 120,497,074 bp
  • A to T, chromosome 12 at 83,547,931 bp
  • A to T, chromosome 12 at 116,229,571 bp
  • T to A, chromosome 13 at 62,371,843 bp
  • G to T, chromosome 14 at 30,042,342 bp
  • A to T, chromosome 14 at 56,613,817 bp
  • A to T, chromosome 14 at 64,075,722 bp
  • G to T, chromosome 14 at 86,966,446 bp
  • G to T, chromosome 15 at 55,548,219 bp
  • T to A, chromosome 15 at 78,918,319 bp
  • T to A, chromosome 15 at 89,275,487 bp
  • A to G, chromosome 16 at 17,858,345 bp
  • C to T, chromosome 16 at 55,914,844 bp
  • A to G, chromosome 17 at 13,888,891 bp
  • A to T, chromosome 17 at 14,368,971 bp
  • A to G, chromosome 17 at 20,688,213 bp
  • A to G, chromosome 17 at 27,113,579 bp
  • C to T, chromosome 17 at 34,932,766 bp
  • A to C, chromosome 17 at 57,509,479 bp
  • C to A, chromosome 18 at 7,265,156 bp
  • A to G, chromosome 18 at 12,525,079 bp
  • G to A, chromosome 18 at 33,107,943 bp
  • T to A, chromosome 18 at 34,312,491 bp
  • C to T, chromosome 18 at 36,644,333 bp
  • G to T, chromosome 18 at 39,357,698 bp
  • T to A, chromosome 18 at 60,963,959 bp
  • C to T, chromosome 18 at 68,339,364 bp
  • T to C, chromosome 18 at 89,263,259 bp
  • A to G, chromosome 19 at 6,106,195 bp
  • G to T, chromosome 19 at 11,973,834 bp
  • A to G, chromosome 19 at 28,964,320 bp
  • T to A, chromosome 19 at 31,084,864 bp
  • T to A, chromosome 19 at 37,407,003 bp
  • T to C, chromosome 19 at 56,470,837 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2291 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040290-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.