Strain Name:
C57BL/6J-MtgxR2426Btlr/Mmmh
Stock Number:
040388-MU
Citation ID:
RRID:MMRRC_040388-MU
Other Names:
R2426 (G1), C57BL/6J-MtgxR2426Btlr
Major Collection:

Gene Information

Gene Symbol: Mon1b [MGI:1923231] (Mus musculus (mouse))
Name: MON1 homolog B, secretory traffciking associated
Synonyms: 5033413H12Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Aldh1l1 [MGI:1340024] (Mus musculus (mouse))
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Frem2 [MGI:2444465] (Mus musculus (mouse))
Name: Fras1 related extracellular matrix protein 2
Synonyms: ne, b2b1562Clo, 6030440P17Rik, my, 8430406N05Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Meis1 [MGI:104717] (Mus musculus (mouse))
Name: Meis homeobox 1
Synonyms: C530044H18Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Dst [MGI:104627] (Mus musculus (mouse))
Name: dystonin
Synonyms: BPAG1, Bpag1, nmf339, bullous pemphigoid antigen 1, Macf2, ah, A830042E19Rik, athetoid, nmf203, bullous pemphigoid antigen 1, BPAG1-n, 2310001O04Rik, Bpag
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Zbtb8a [MGI:1920930] (Mus musculus (mouse))
Name: zinc finger and BTB domain containing 8a
Synonyms: 2410081M15Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Gdi2 [MGI:99845] (Mus musculus (mouse))
Name: guanosine diphosphate (GDP) dissociation inhibitor 2
Synonyms: GDI-B, GDIB, GDI beta, Gdi3
Chromosome: 13
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,192,812 bp
  • C to A, chromosome 1 at 40,446,619 bp
  • A to G, chromosome 1 at 59,130,057 bp
  • C to A, chromosome 1 at 78,817,971 bp
  • T to G, chromosome 1 at 175,963,976 bp
  • A to G, chromosome 1 at 176,774,635 bp
  • A to G, chromosome 1 at 192,693,960 bp
  • T to A, chromosome 2 at 4,529,862 bp
  • A to T, chromosome 2 at 22,600,200 bp
  • C to T, chromosome 2 at 24,019,155 bp
  • T to A, chromosome 2 at 29,067,185 bp
  • A to G, chromosome 2 at 52,169,053 bp
  • A to G, chromosome 2 at 67,514,471 bp
  • T to G, chromosome 2 at 84,770,219 bp
  • G to A, chromosome 2 at 118,715,649 bp
  • T to C, chromosome 2 at 129,208,230 bp
  • A to T, chromosome 2 at 155,299,082 bp
  • A to G, chromosome 3 at 28,646,681 bp
  • C to T, chromosome 3 at 53,535,510 bp
  • A to T, chromosome 4 at 59,099,443 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • G to A, chromosome 4 at 86,156,788 bp
  • T to A, chromosome 4 at 115,571,016 bp
  • A to G, chromosome 4 at 129,360,219 bp
  • A to T, chromosome 4 at 130,818,803 bp
  • G to T, chromosome 4 at 143,111,750 bp
  • A to C, chromosome 5 at 21,522,137 bp
  • A to T, chromosome 5 at 24,386,994 bp
  • A to T, chromosome 5 at 50,009,449 bp
  • A to T, chromosome 5 at 106,847,653 bp
  • T to C, chromosome 5 at 137,388,992 bp
  • A to T, chromosome 5 at 139,771,814 bp
  • A to T, chromosome 5 at 145,220,940 bp
  • A to T, chromosome 6 at 85,118,631 bp
  • A to G, chromosome 6 at 90,598,284 bp
  • A to G, chromosome 6 at 148,355,161 bp
  • A to G, chromosome 7 at 11,165,095 bp
  • T to C, chromosome 7 at 76,421,902 bp
  • A to G, chromosome 7 at 90,228,431 bp
  • G to T, chromosome 7 at 120,283,223 bp
  • A to G, chromosome 7 at 127,487,339 bp
  • T to C, chromosome 8 at 8,667,424 bp
  • T to G, chromosome 8 at 21,702,637 bp
  • T to A, chromosome 8 at 45,317,776 bp
  • T to C, chromosome 8 at 85,231,064 bp
  • A to G, chromosome 8 at 105,357,659 bp
  • G to A, chromosome 8 at 113,639,120 bp
  • G to A, chromosome 9 at 8,900,717 bp
  • T to C, chromosome 9 at 13,706,498 bp
  • G to A, chromosome 9 at 28,903,367 bp
  • A to T, chromosome 9 at 35,670,480 bp
  • A to T, chromosome 9 at 57,027,428 bp
  • T to C, chromosome 9 at 100,845,116 bp
  • A to T, chromosome 9 at 106,914,541 bp
  • A to G, chromosome 10 at 42,563,485 bp
  • T to C, chromosome 10 at 129,269,266 bp
  • A to T, chromosome 11 at 18,988,356 bp
  • T to A, chromosome 11 at 23,576,801 bp
  • A to T, chromosome 11 at 48,946,063 bp
  • G to A, chromosome 11 at 57,493,080 bp
  • A to T, chromosome 11 at 69,827,086 bp
  • A to G, chromosome 11 at 72,915,265 bp
  • T to C, chromosome 11 at 100,086,366 bp
  • T to G, chromosome 13 at 3,562,034 bp
  • G to A, chromosome 14 at 20,564,940 bp
  • G to T, chromosome 14 at 27,384,181 bp
  • A to T, chromosome 15 at 55,906,179 bp
  • G to T, chromosome 17 at 12,726,657 bp
  • G to T, chromosome 17 at 35,424,937 bp
  • G to A, chromosome 17 at 56,172,030 bp
  • T to A, chromosome 18 at 20,336,804 bp
  • G to T, chromosome 18 at 43,735,222 bp
  • A to T, chromosome 19 at 9,002,851 bp
  • A to T, chromosome 19 at 48,722,925 bp
  • T to C, chromosome X at 145,476,291 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R2426 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    040388-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.