Strain Name:
C57BL/6J-MtgxR2473Btlr/Mmmh
Stock Number:
040404-MU
Citation ID:
RRID:MMRRC_040404-MU
Other Names:
R2473 (G1), C57BL/6J-MtgxR2473Btlr
Major Collection:

Strain Information

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: 9430004D19Rik, C030040A15Rik, OTTMUSG00000012483
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 109241
Homologene: 81861
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Sf3b1
Name: splicing factor 3b, subunit 1
Synonyms: SAP155, Prp10, SF3b155, 2810001M05Rik, Targ4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 81898
Homologene: 6696
Calcr
Name: calcitonin receptor
Synonyms: Clr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12311
HGNC: HGNC:1440
Homologene: 1320
Mxra8
Name: matrix-remodelling associated 8
Synonyms: 1200013A08Rik, limitrin, DICAM, Asp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74761
HGNC: HGNC:7542
Homologene: 11500
Mgp
Name: matrix Gla protein
Synonyms: Mglap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17313
HGNC: HGNC:7060
Homologene: 693
Marveld2
Name: MARVEL (membrane-associating) domain containing 2
Synonyms: Mrvldc2, Tricellulin, Tric, Tric-c, Tric-b, Tric-a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218518
VEGA: 13
Homologene: 27037
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Slc16a12
Name: solute carrier family 16 (monocarboxylic acid transporters), member 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240638
Homologene: 130007
Ttc39a
Name: tetratricopeptide repeat domain 39A
Synonyms: 4922503N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230603
Homologene: 17739
Cacnb2
Name: calcium channel, voltage-dependent, beta 2 subunit
Synonyms: Cchb2, Cavbeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12296
HGNC: HGNC:1402
Homologene: 75191
Mamdc4
Name: MAM domain containing 4
Synonyms: LOC381352
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 381352
Homologene: 17102
Or5p70
Name: olfactory receptor family 5 subfamily P member 70
Synonyms: GA_x6K02T2PBJ9-10725148-10726140, MOR204-37, Olfr495
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258361
Homologene: 133600
Mucl2
Name: mucin-like 2
Synonyms: Spt-1, Spt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20770
Homologene: 137219
Myom3
Name: myomesin family, member 3
Synonyms: 8430427K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242702
Homologene: 19432
Or4g16
Name: olfactory receptor family 4 subfamily G member 16
Synonyms: GA_x6K02T2Q125-72357646-72358581, MOR245-12, Olfr1279
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258388
Homologene: 121520
Sec14l4
Name: SEC14-like lipid binding 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103655
Homologene: 68807
Atp8b4
Name: ATPase, class I, type 8B, member 4
Synonyms: Im
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241633
Homologene: 133162
Slc35c1
Name: solute carrier family 35, member C1
Synonyms: FUCT1, E430007K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228368
Homologene: 41258
Cyp3a16
Name: cytochrome P450, family 3, subfamily a, polypeptide 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13114
Homologene: 133568
Habp2
Name: hyaluronic acid binding protein 2
Synonyms: FSAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226243
HGNC: HGNC:4798
Homologene: 3050
Ephb4
Name: Eph receptor B4
Synonyms: MDK2, Myk1, Htk, Tyro11, b2b2412Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13846
HGNC: HGNC:3395
Homologene: 20939
Or2t46
Name: olfactory receptor family 2 subfamily T member 46
Synonyms: GA_x6K02T2NKPP-844642-843680, MOR275-5, MOR275-11_p, Olfr325
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258261
Homologene: 133015
Polm
Name: polymerase (DNA directed), mu
Synonyms: Tdt-N, B230309I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 54125
HGNC: HGNC:9185
Homologene: 41170
Zfp414
Name: zinc finger protein 414
Synonyms: 0610030H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328801
Homologene: 12264
Six4
Name: sine oculis-related homeobox 4
Synonyms: AREC3, TrexBF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20474
Homologene: 69089
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 54,999,626 bp
  • C to T, chromosome 1 at 78,122,590 bp
  • G to A, chromosome 2 at 14,984,314 bp
  • C to A, chromosome 2 at 25,566,332 bp
  • T to A, chromosome 2 at 49,279,341 bp
  • T to A, chromosome 2 at 92,454,753 bp
  • T to C, chromosome 2 at 111,306,891 bp
  • T to G, chromosome 2 at 126,358,894 bp
  • A to T, chromosome 4 at 109,442,239 bp
  • A to T, chromosome 4 at 135,806,024 bp
  • T to C, chromosome 4 at 155,842,043 bp
  • T to A, chromosome 5 at 137,365,700 bp
  • T to A, chromosome 5 at 145,455,594 bp
  • T to A, chromosome 6 at 3,711,439 bp
  • T to C, chromosome 6 at 136,873,164 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • C to T, chromosome 7 at 108,395,504 bp
  • G to A, chromosome 9 at 79,643,855 bp
  • T to A, chromosome 11 at 4,043,359 bp
  • T to C, chromosome 11 at 5,829,881 bp
  • A to G, chromosome 11 at 58,581,575 bp
  • A to G, chromosome 12 at 73,104,175 bp
  • C to T, chromosome 13 at 100,597,321 bp
  • C to G, chromosome 15 at 103,897,362 bp
  • CAAACTCTTCCGA to CAAACTCTTCCGAAACTCTTCCGA, chromosome 17 at 33,630,577 bp
  • C to T, chromosome 19 at 34,677,277 bp
  • G to A, chromosome 19 at 56,288,032 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2473 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040404-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.