Strain Name:
C57BL/6J-MtgxR2859Btlr/Mmmh
Stock Number:
040449-MU
Citation ID:
RRID:MMRRC_040449-MU
Other Names:
R2859 (G1), C57BL/6J-MtgxR2859Btlr
Major Collection:

Gene Information

Thbs4
Name: thrombospondin 4
Synonyms: TSP4, TSP-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 21828
Homologene: 20691
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: E430024A07Rik, Mthfd, DCS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Ntmt1
Name: N-terminal Xaa-Pro-Lys N-methyltransferase 1
Synonyms: 2610205E22Rik, Mettl11a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66617
Homologene: 5439
Ulk1
Name: unc-51 like kinase 1
Synonyms: Unc51.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22241
Homologene: 2640
Setx
Name: senataxin
Synonyms: A930037J23Rik, Als4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 269254
HGNC: HGNC:445
Homologene: 41003
Scaf8
Name: SR-related CTD-associated factor 8
Synonyms: Rbm16, A930036P18Rik, A630086M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 106583
VEGA: 17
Homologene: 8928
Mastl
Name: microtubule associated serine/threonine kinase-like
Synonyms: 2700091H24Rik, THC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67121
Homologene: 12086
Robo3
Name: roundabout guidance receptor 3
Synonyms: Rig-1, Rig1, Robo3a, Rbig1, Robo3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 19649
Homologene: 32119
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 404710
Homologene: 26400
Ppp4r3a
Name: protein phosphatase 4 regulatory subunit 3A
Synonyms: Smek1, 1110034C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 68734
VEGA: 12
Homologene: 69510
Abca17
Name: ATP-binding cassette, sub-family A (ABC1), member 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 381072
Homologene: 86807
Cadm3
Name: cell adhesion molecule 3
Synonyms: Necl-1, Igsf4b, BIgR, Necl1, Tsll1, SynCAM3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 94332
Homologene: 10919
Cuzd1
Name: CUB and zona pellucida-like domains 1
Synonyms: Itmap1, UTCZP, ERG-1, UO-44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16433
Homologene: 7389
Patl1
Name: protein associated with topoisomerase II homolog 1 (yeast)
Synonyms: Pat1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 225929
VEGA: 19
Homologene: 82269
Parm1
Name: prostate androgen-regulated mucin-like protein 1
Synonyms: 9130213B05Rik, 2210012L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231440
Homologene: 41036
Cdh23
Name: cadherin 23 (otocadherin)
Synonyms: USH1D, mdfw, ahl, nmf112, nmf181, nmf252, 4930542A03Rik, sals, bob
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22295
Homologene: 11142
Amfr
Name: autocrine motility factor receptor
Synonyms: gp78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 23802
HGNC: HGNC:463
Homologene: 888
Ism2
Name: isthmin 2
Synonyms: LOC217738, Thsd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217738
Homologene: 83509
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Gm20114, Shn-2, Schnurri-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Fam71b
Name: family with sequence similarity 71, member B
Synonyms: OTTMUSG00000005491
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 432552
Homologene: 51067
Mink1
Name: misshapen-like kinase 1 (zebrafish)
Synonyms: Ysk2, Map4k6, MINK, Misshapen/NIKs-related kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 50932
Homologene: 56762
Trim34b
Name: tripartite motif-containing 34B
Synonyms: Gm15134, Trim34-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 434218
Homologene: 128238
Alox5ap
Name: arachidonate 5-lipoxygenase activating protein
Synonyms: Flap, arachidonate 5 lipoxygenase activating protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11690
HGNC: HGNC:436
Homologene: 1231
Cd109
Name: CD109 antigen
Synonyms: Gov platelet alloantigens, 9930012E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235505
Homologene: 25183
Fmo9
Name: flavin containing monooxygenase 9
Synonyms: 4831428F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240894
Homologene: 134192
Olfr340
Name: olfactory receptor 340
Synonyms: MOR136-1, GA_x6K02T2NLDC-33147742-33148680
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258953
Homologene: 74159
Vmn2r104
Name: vomeronasal 2, receptor 104
Synonyms: V2r7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 22313
Homologene: 129751
Vrk2
Name: vaccinia related kinase 2
Synonyms: 2810003O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69922
Homologene: 55966
Rbm17
Name: RNA binding motif protein 17
Synonyms: 2700027J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 76938
Homologene: 13162
Olfr139
Name: olfactory receptor 139
Synonyms: MOR255-2, M5, GA_x6K02T2P1NL-4202012-4201065
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 259005
Homologene: 77386
Zswim3
Name: zinc finger SWIM-type containing 3
Synonyms: 4921517A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67538
Homologene: 15436
Samhd1
Name: SAM domain and HD domain, 1
Synonyms: E330031J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 56045
Homologene: 9160
Ceacam1
Name: carcinoembryonic antigen-related cell adhesion molecule 1
Synonyms: Cc1, C-CAM, Cea-7, Hv-2, CD66a, MHVR1, mmCGM1, mCEA1, mmCGM2, Cea-1, Cea1, Mhv-1, Bgp1, Bgp, Cea7, Hv2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 26365
HGNC: HGNC:1814
Homologene: 128630
Fibin
Name: fin bud initiation factor homolog (zebrafish)
Synonyms: Fibin, 1110018M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67606
Homologene: 12163
Ehd2
Name: EH-domain containing 2
Synonyms: C130052H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 259300
HGNC: HGNC:3243
Homologene: 22825
Olfr401
Name: olfactory receptor 401
Synonyms: GA_x6K02T2P1NL-4278037-4278984, MOR255-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 258701
HGNC: HGNC:8282
Homologene: 73922
Olfr311
Name: olfactory receptor 311
Synonyms: MOR222-1, GA_x6K02T2NKPP-565870-564944
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 258530
Homologene: 77375
C87977
Name: expressed sequence C87977
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 97187
Homologene: 103830
Phospho2
Name: phosphatase, orphan 2
Synonyms: Phos2, 1700048E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 73373
Homologene: 41756
Mrgprb4
Name: MAS-related GPR, member B4
Synonyms: MrgB4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233230
Homologene: 115575
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 166,673,667 bp
  • A to G, chromosome 1 at 173,346,545 bp
  • A to G, chromosome 2 at 11,590,704 bp
  • C to A, chromosome 2 at 23,139,967 bp
  • GTGGCT to GT, chromosome 2 at 29,154,061 bp
  • A to G, chromosome 2 at 30,822,365 bp
  • T to C, chromosome 2 at 36,453,130 bp
  • T to C, chromosome 2 at 69,795,851 bp
  • C to T, chromosome 2 at 110,362,197 bp
  • A to G, chromosome 2 at 157,106,229 bp
  • T to C, chromosome 2 at 164,820,389 bp
  • C to A, chromosome 3 at 88,107,596 bp
  • A to G, chromosome 4 at 144,209,622 bp
  • A to G, chromosome 5 at 91,594,306 bp
  • A to G, chromosome 5 at 110,794,629 bp
  • T to C, chromosome 5 at 149,285,439 bp
  • A to T, chromosome 7 at 15,964,129 bp
  • T to A, chromosome 7 at 25,474,017 bp
  • T to A, chromosome 7 at 48,198,336 bp
  • A to T, chromosome 7 at 104,336,232 bp
  • C to T, chromosome 7 at 131,316,134 bp
  • A to T, chromosome 8 at 94,005,214 bp
  • C to A, chromosome 9 at 37,428,104 bp
  • CATTTATTTATTTATTTATTTATTTATTTATTTAT to CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT, chromosome 9 at 78,712,500 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • C to T, chromosome 10 at 60,382,653 bp
  • C to A, chromosome 11 at 26,483,324 bp
  • T to C, chromosome 11 at 46,405,212 bp
  • T to A, chromosome 11 at 58,841,882 bp
  • T to C, chromosome 11 at 70,612,508 bp
  • C to T, chromosome 11 at 74,044,827 bp
  • T to C, chromosome 11 at 74,121,982 bp
  • T to C, chromosome 12 at 76,288,925 bp
  • T to C, chromosome 12 at 87,299,663 bp
  • T to C, chromosome 12 at 101,042,647 bp
  • A to G, chromosome 13 at 92,790,708 bp
  • T to C, chromosome 17 at 3,193,004 bp
  • T to A, chromosome 17 at 20,048,193 bp
  • T to C, chromosome 17 at 24,281,314 bp
  • T to C, chromosome 19 at 11,923,831 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2859 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040449-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.