Strain Name:
C57BL/6J-MtgxR2888Btlr/Mmmh
Stock Number:
040476-MU
Citation ID:
RRID:MMRRC_040476-MU
Other Names:
R2888 (G1), C57BL/6J-MtgxR2888Btlr
Major Collection:

Gene Information

Etv4
Name: ets variant 4
Synonyms: Pea-3, Pea3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18612
HGNC: HGNC:3493
Homologene: 1504
Rtn4
Name: reticulon 4
Synonyms: 1110020G17Rik, C130026I10Rik, NOGO, NgA, Nogo-B, Nogo-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68585
Homologene: 10743
Tbc1d5
Name: TBC1 domain family, member 5
Synonyms: 1600014N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72238
VEGA: 17
Homologene: 8834
Smoc2
Name: SPARC related modular calcium binding 2
Synonyms: 1700056C05Rik, 5430426J21Rik, Smoc2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 64074
VEGA: 17
Homologene: 11150
Umps
Name: uridine monophosphate synthetase
Synonyms: 1700095D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22247
Homologene: 319
Ahi1
Name: Abelson helper integration site 1
Synonyms: Ahi-1, 1700015F03Rik, D10Bwg0629e, Jouberin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52906
VEGA: 10
Homologene: 9762
Dsp
Name: desmoplakin
Synonyms: DP, 2300002E22Rik, 5730453H04Rik, rul
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 109620
HGNC: HGNC:3052
Homologene: 37922
Rbm39
Name: RNA binding motif protein 39
Synonyms: caper, 2310040E03Rik, 1500012C14Rik, Rnpc2, B330012G18Rik, Caper alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 170791
Homologene: 136465
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20743
VEGA: 19
Homologene: 48482
Aimp2
Name: aminoacyl tRNA synthetase complex-interacting multifunctional protein 2
Synonyms: Jtv1, Aimp2(p38)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231872
Homologene: 4594
Zfhx2
Name: zinc finger homeobox 2
Synonyms: zfh-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239102
Homologene: 52657
Pkd1l1
Name: polycystic kidney disease 1 like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 171395
Homologene: 51376
Prol1
Name: proline rich, lacrimal 1
Synonyms: Muc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17830
Tsc2
Name: TSC complex subunit 2
Synonyms: tuberin, Nafld, tuberous sclerosis 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22084
VEGA: 17
Homologene: 462
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Ppp1r3a
Name: protein phosphatase 1, regulatory subunit 3A
Synonyms: GM, RGL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 140491
HGNC: HGNC:9291
Homologene: 48124
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 545030
Homologene: 83473
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC9, MUC5, 2300002I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Cacna1i
Name: calcium channel, voltage-dependent, alpha 1I subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239556
HGNC: HGNC:1396
Homologene: 69331
Kansl1l
Name: KAT8 regulatory NSL complex subunit 1-like
Synonyms: C430010P07Rik, 1110028C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68691
Homologene: 27376
Plekha4
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
Synonyms: PEPP1, 2410005C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69217
Homologene: 10848
Myo5b
Name: myosin VB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17919
HGNC: HGNC:7603
Homologene: 49481
Mug1
Name: murinoglobulin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17836
Homologene: 136663
Lamp1
Name: lysosomal-associated membrane protein 1
Synonyms: CD107a, Lamp-1, Perk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16783
HGNC: HGNC:6499
Homologene: 4061
Pramel23
Name: PRAME like 23
Synonyms: Gm13089
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 277667
Vmn2r13
Name: vomeronasal 2, receptor 13
Synonyms: Gm4867
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231589
Homologene: 129606
Phex
Name: phosphate regulating endopeptidase homolog, X-linked
Synonyms: HPDR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18675
HGNC: HGNC:8918
Homologene: 37310
Gusb
Name: glucuronidase, beta
Synonyms: g, Gur, Gut, asd, Gus-u, Gus-t, Gus-s, Gus-r, adipose storage deficiency, Gus
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 110006
HGNC: HGNC:4696
Homologene: 37271
Extl2
Name: exostosin-like glycosyltransferase 2
Synonyms: 3000001D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 58193
HGNC: HGNC:3516
Homologene: 1102
Atp8b2
Name: ATPase, class I, type 8B, member 2
Synonyms: Id
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 54667
Homologene: 23226
Olfr325
Name: olfactory receptor 325
Synonyms: GA_x6K02T2NKPP-844642-843680, MOR275-5, MOR275-11_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258261
Homologene: 133015
Acd
Name: adrenocortical dysplasia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 497652
Homologene: 23391
Krtap4-9
Name: keratin associated protein 4-9
Synonyms: OTTMUSG00000002198
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 665998
Gm13318
Name: predicted gene 13318
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Zfp511
Name: zinc finger protein 511
Synonyms: 2410004P19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69752
Homologene: 42950
Llcfc1
Name: LLLL and CFNLAS motif containing 1
Synonyms: 1700034O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76606
Homologene: 81932
Slc35a5
Name: solute carrier family 35, member A5
Synonyms: D16Ertd450e, 1010001J06Rik, D730043G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74102
Homologene: 9930
4930480E11Rik
Name: RIKEN cDNA 4930480E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 74910
Homologene: 136363
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 66,724,605 bp
  • A to T, chromosome 2 at 16,074,757 bp
  • C to T, chromosome 2 at 156,167,583 bp
  • T to C, chromosome 3 at 89,958,293 bp
  • T to C, chromosome 3 at 116,027,257 bp
  • G to T, chromosome 4 at 143,696,890 bp
  • C to A, chromosome 5 at 88,328,309 bp
  • T to C, chromosome 5 at 109,191,974 bp
  • T to C, chromosome 5 at 130,000,502 bp
  • T to C, chromosome 5 at 143,909,735 bp
  • T to G, chromosome 6 at 14,718,249 bp
  • A to T, chromosome 6 at 41,684,603 bp
  • T to A, chromosome 6 at 121,881,843 bp
  • C to T, chromosome 6 at 146,171,293 bp
  • C to T, chromosome 7 at 45,538,244 bp
  • A to C, chromosome 7 at 140,039,382 bp
  • G to A, chromosome 7 at 141,861,554 bp
  • T to A, chromosome 8 at 13,173,891 bp
  • A to G, chromosome 8 at 105,698,838 bp
  • T to A, chromosome 10 at 20,968,978 bp
  • T to A, chromosome 11 at 8,947,251 bp
  • T to C, chromosome 11 at 29,693,687 bp
  • T to C, chromosome 11 at 58,581,162 bp
  • C to A, chromosome 11 at 99,785,419 bp
  • A to T, chromosome 11 at 101,771,515 bp
  • C to A, chromosome 13 at 38,192,248 bp
  • C to A, chromosome 14 at 33,109,519 bp
  • T to C, chromosome 14 at 55,064,803 bp
  • A to T, chromosome 15 at 80,374,767 bp
  • A to T, chromosome 16 at 33,963,870 bp
  • A to G, chromosome 16 at 45,151,560 bp
  • T to C, chromosome 17 at 14,397,625 bp
  • A to G, chromosome 17 at 24,631,995 bp
  • T to A, chromosome 17 at 50,935,549 bp
  • G to C, chromosome 18 at 74,762,618 bp
  • A to G, chromosome 19 at 4,748,636 bp
  • A to T, chromosome X at 78,370,682 bp
  • T to C, chromosome X at 157,310,958 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2888 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040476-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.