Strain Name:
C57BL/6J-MtgxR2964Btlr/Mmmh
Stock Number:
040520-MU
Citation ID:
RRID:MMRRC_040520-MU
Other Names:
R2964 (G1), C57BL/6J-MtgxR2964Btlr
Major Collection:

Gene Information

Kif3a
Name: kinesin family member 3A
Synonyms: Kifl, N-4 kinesin, Kif3, kinesin-II subunit, Kns3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16568
HGNC: HGNC:6319
Homologene: 38266
Nup188
Name: nucleoporin 188
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227699
Homologene: 45683
Dbi
Name: diazepam binding inhibitor
Synonyms: diazepam-binding inhibitor, Acbp, EP, endozepine
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13167
HGNC: HGNC:2690
Homologene: 39086
2610021A01Rik
Name: RIKEN cDNA 2610021A01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668572
Zcchc8
Name: zinc finger, CCHC domain containing 8
Synonyms: 5730565F05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70650
Homologene: 32349
Mdc1
Name: mediator of DNA damage checkpoint 1
Synonyms: NFBD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240087
Homologene: 67092
Gtf2ird1
Name: general transcription factor II I repeat domain-containing 1
Synonyms: WBSCR11, Tg(Alb1-Myc)166.8Sst, c-myc line 166.8, Alb/c-myc line 166.8, Alb-c-myc line 166.8, ESTM9, Cream1, GTF3, MusTRD1, binding factor for early enhancer, BEN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57080
HGNC: HGNC:4661
Homologene: 4158
Nav2
Name: neuron navigator 2
Synonyms: HELAD1, RAINB2, 5330421F07Rik, POMFIL2, Unc53H2, Rainb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78286
Homologene: 52330
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: MAP1C, Swl, 9930018I23Rik, Dnec1, dynein heavy chain, retrograde transport, Dnchc1, Loa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Lrp6
Name: low density lipoprotein receptor-related protein 6
Synonyms: Cd, ska26, skam26Jus, skax26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16974
HGNC: HGNC:6698
Homologene: 1747
Oprm1
Name: opioid receptor, mu 1
Synonyms: MOP receptor, mor, MOR-1, Oprm, muOR, MOP-R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18390
HGNC: HGNC:8156
Homologene: 37368
Ing1
Name: inhibitor of growth family, member 1
Synonyms: p33Ing1, mING1h, 2610028J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26356
HGNC: HGNC:6062
Homologene: 40119
Ap1s1
Name: adaptor protein complex AP-1, sigma 1
Synonyms: AP19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11769
HGNC: HGNC:559
Homologene: 20342
Fabp3
Name: fatty acid binding protein 3, muscle and heart
Synonyms: Fabp3, Fabph4, Fabph-1, Fabph-4, Mdgi, H-FABP, Fabph1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14077
HGNC: HGNC:3557
Homologene: 68379
Rasal1
Name: RAS protein activator like 1 (GAP1 like)
Synonyms: MRASAL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19415
HGNC: HGNC:9873
Homologene: 3423
Chrna2
Name: cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal)
Synonyms: Acra-2, Acra2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110902
HGNC: HGNC:1956
Homologene: 20193
Pigr
Name: polymeric immunoglobulin receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18703
HGNC: HGNC:8968
Homologene: 1984
Chsy1
Name: chondroitin sulfate synthase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269941
Homologene: 8950
Fstl3
Name: follistatin-like 3
Synonyms: E030038F23Rik, Flrg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 83554
HGNC: HGNC:3973
Homologene: 4280
Acsl3
Name: acyl-CoA synthetase long-chain family member 3
Synonyms: 2610510B12Rik, C85929, Facl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74205
HGNC: HGNC:3570
Homologene: 3278
Sdccag8
Name: serologically defined colon cancer antigen 8
Synonyms: CCCAP, 5730470G24Rik, 2700048G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76816
Homologene: 4839
Mnd1
Name: meiotic nuclear divisions 1
Synonyms: 2610034E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76915
Homologene: 5890
H2-T22
Name: histocompatibility 2, T region locus 22
Synonyms: H-2T22, H-2T17, H2-T17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15039
Homologene: 79525
Col13a1
Name: collagen, type XIII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12817
HGNC: HGNC:2190
Homologene: 22421
Myo3a
Name: myosin IIIA
Synonyms: 9030416P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 667663
HGNC: HGNC:7601
Homologene: 49486
Cul9
Name: cullin 9
Synonyms: 1810035I07Rik, Parc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78309
Homologene: 56696
Clca3a2
Name: chloride channel accessory 3A2
Synonyms: Clca2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80797
Homologene: 77224
Slc4a5
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 5
Synonyms: C330016K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232156
Homologene: 31125
Ltf
Name: lactotransferrin
Synonyms: lactoferrin, Lf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17002
HGNC: HGNC:6720
Homologene: 1754
Olfr340
Name: olfactory receptor 340
Synonyms: GA_x6K02T2NLDC-33147742-33148680, MOR136-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258953
Homologene: 74159
Olfr1453
Name: olfactory receptor 1453
Synonyms: GA_x6K02T2RE5P-3357666-3356743, MOR202-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258695
Homologene: 120159
Cwh43
Name: cell wall biogenesis 43 C-terminal homolog
Synonyms: C130090K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231293
Homologene: 5474
Nlrp4d
Name: NLR family, pyrin domain containing 4D
Synonyms: Nalp4d, Nalp-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384752
Gsdma2
Name: gasdermin A2
Synonyms: Gsdm2, 2210411P14Rik, 2210006M16Rik, Gsdml2, 2210009F20Rik, 2200001G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76758
Fbxw21
Name: F-box and WD-40 domain protein 21
Synonyms: E330009P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320082
Homologene: 110776
Mdga1
Name: MAM domain containing glycosylphosphatidylinositol anchor 1
Synonyms: Mamdc3, 1200011I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74762
Homologene: 17780
Asprv1
Name: aspartic peptidase, retroviral-like 1
Synonyms: SASPase, Taps, TPA-induced aspartic proteinase-like, 2300003P22Rik, SASP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67855
Homologene: 23582
Hrh4
Name: histamine receptor H4
Synonyms: H4R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225192
VEGA: 18
Homologene: 11002
Pnpla2
Name: patatin-like phospholipase domain containing 2
Synonyms: 1110001C14Rik, Atgl, 0610039C21Rik, desnutrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 66853
Homologene: 10687
Gpr45
Name: G protein-coupled receptor 45
Synonyms: 9230112G11Rik, PSP24alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 93690
HGNC: HGNC:4503
Homologene: 5228
Sp110
Name: Sp110 nuclear body protein
Synonyms: 5830484A20Rik, 5031415C07Rik, 52kDa, Ipr1, Ifi75
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192
Gm10542
Name: predicted gene 10542
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Acox3
Name: acyl-Coenzyme A oxidase 3, pristanoyl
Synonyms: pristanoyl-CoA oxidase, EST-s59, PCOX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80911
HGNC: HGNC:121
Homologene: 37792
Pth
Name: parathyroid hormone
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19226
HGNC: HGNC:9606
Homologene: 266
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 43,032,508 bp
  • A to G, chromosome 1 at 78,694,294 bp
  • A to C, chromosome 1 at 85,577,329 bp
  • C to T, chromosome 1 at 120,120,116 bp
  • G to A, chromosome 1 at 130,841,535 bp
  • A to T, chromosome 1 at 176,948,371 bp
  • T to C, chromosome 2 at 22,340,256 bp
  • T to A, chromosome 2 at 30,325,346 bp
  • T to C, chromosome 2 at 36,452,767 bp
  • C to A, chromosome 3 at 84,134,109 bp
  • T to C, chromosome 3 at 144,817,137 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • T to C, chromosome 5 at 35,605,267 bp
  • T to C, chromosome 5 at 73,408,336 bp
  • T to A, chromosome 5 at 120,671,620 bp
  • A to G, chromosome 5 at 123,720,867 bp
  • T to C, chromosome 5 at 134,357,684 bp
  • C to A, chromosome 5 at 134,415,121 bp
  • T to C, chromosome 5 at 137,037,503 bp
  • C to T, chromosome 6 at 83,296,669 bp
  • T to A, chromosome 6 at 86,628,366 bp
  • T to C, chromosome 6 at 134,467,526 bp
  • G to T, chromosome 7 at 10,378,329 bp
  • C to T, chromosome 7 at 41,626,405 bp
  • C to T, chromosome 7 at 49,557,032 bp
  • G to A, chromosome 7 at 66,172,164 bp
  • T to C, chromosome 7 at 113,385,929 bp
  • C to T, chromosome 7 at 141,458,478 bp
  • T to A, chromosome 8 at 11,561,641 bp
  • A to G, chromosome 9 at 109,145,510 bp
  • G to T, chromosome 9 at 111,028,472 bp
  • T to C, chromosome 10 at 6,788,914 bp
  • G to A, chromosome 10 at 61,961,331 bp
  • T to C, chromosome 10 at 79,781,223 bp
  • A to T, chromosome 11 at 53,578,930 bp
  • T to C, chromosome 11 at 98,657,259 bp
  • G to A, chromosome 12 at 110,641,026 bp
  • T to C, chromosome 14 at 66,149,368 bp
  • A to T, chromosome 17 at 29,852,468 bp
  • A to T, chromosome 17 at 35,853,637 bp
  • A to G, chromosome 17 at 36,040,645 bp
  • A to G, chromosome 17 at 46,502,228 bp
  • T to C, chromosome 18 at 13,022,369 bp
  • A to T, chromosome 18 at 44,204,695 bp
  • C to T, chromosome 19 at 13,028,048 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2964 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040520-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.