Strain Name:
C57BL/6J-MtgxR3085Btlr/Mmmh
Stock Number:
040574-MU
Citation ID:
RRID:MMRRC_040574-MU
Other Names:
R3085 (G1), C57BL/6J-MtgxR3085Btlr
Major Collection:

Gene Information

Sash1
Name: SAM and SH3 domain containing 1
Synonyms: A330076K04Rik, 1100001C18Rik, 2500002E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 70097
Homologene: 69182
Wdr73
Name: WD repeat domain 73
Synonyms: 2410008B13Rik, 1200011I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 71968
Homologene: 41899
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: D230004K18Rik, b2b1625.2Clo, Gp330, Megalin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Mthfd1l
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms: 2410004L15Rik, Fthfsdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 270685
Homologene: 56706
Stx12
Name: syntaxin 12
Synonyms: syntaxin 13, Stx13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100226
Homologene: 128192
Dhx9
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 9
Synonyms: NDHII, NDH II, RHA, RNA helicase, nuclear DNA helicase II, leukophysin, Ddx9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 13211
HGNC: HGNC:2750
Homologene: 1039
Orc4
Name: origin recognition complex, subunit 4
Synonyms: mMmORC4, Orc4P, Orc4l, Orc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 26428
HGNC: HGNC:8490
Homologene: 8059
Exog
Name: endo/exonuclease (5'-3'), endonuclease G-like
Synonyms: Endogl1, ENDOGL2, ENGL-a, ENGL-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208194
VEGA: 9
HGNC: HGNC:3347
Homologene: 3745
Cenpc1
Name: centromere protein C1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 12617
HGNC: HGNC:1854
Homologene: 1371
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, Sci, A230102L03Rik, Atbf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Ppm1b
Name: protein phosphatase 1B, magnesium dependent, beta isoform
Synonyms: PP2CB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 19043
VEGA: 17
HGNC: HGNC:9276
Homologene: 2027
Zc3hc1
Name: zinc finger, C3HC type 1
Synonyms: 1110054L24Rik, Nipa, HSPC216
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232679
Homologene: 32315
Rnf125
Name: ring finger protein 125
Synonyms: 4930553F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 67664
VEGA: 18
Adrm1
Name: adhesion regulating molecule 1
Synonyms: Gp110, 1110063P18Rik, 2510006J17Rik, Rpn13, ARM-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 56436
Homologene: 10513
Zkscan5
Name: zinc finger with KRAB and SCAN domains 5
Synonyms: Zfp95, hKraba1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22757
Homologene: 8734
Robo1
Name: roundabout guidance receptor 1
Synonyms: DUTT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 19876
Homologene: 2206
Zfp626
Name: zinc finger protein 626
Synonyms: 4933426I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 71163
Dnaaf4
Name: dynein axonemal assembly factor 4
Synonyms: 1700010I24Rik, b2b811Clo, EKN1, Dyx1c1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 67685
Homologene: 12173
Echdc2
Name: enoyl Coenzyme A hydratase domain containing 2
Synonyms: 1300017C12Rik, 2610009M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 52430
Homologene: 23088
Creb3l1
Name: cAMP responsive element binding protein 3-like 1
Synonyms: Oasis, BBF-2 (drosophila) homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 26427
Homologene: 8058
Fcrl5
Name: Fc receptor-like 5
Synonyms: mBXMH2, Fcrh3, BXMAS1-like protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 329693
Homologene: 137404
Havcr1
Name: hepatitis A virus cellular receptor 1
Synonyms: Tim1, TIM-1, KIM-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 171283
Homologene: 136301
Zfp266
Name: zinc finger protein 266
Synonyms: 5330440G10Rik, 5730601F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 77519
Homologene: 105676
Col4a4
Name: collagen, type IV, alpha 4
Synonyms: E130010M05Rik, [a]4(IV)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12829
HGNC: HGNC:2206
Homologene: 20071
Rasgef1b
Name: RasGEF domain family, member 1B
Synonyms: Gpig4, 4732452O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 320292
Homologene: 14860
Smarcc2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
Synonyms: 5930405J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 68094
VEGA: 10
Homologene: 2312
Col4a3
Name: collagen, type IV, alpha 3
Synonyms: alpha3(IV), tumstatin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12828
HGNC: HGNC:2204
Homologene: 68033
Tex15
Name: testis expressed gene 15
Synonyms: 2210014E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 104271
Homologene: 12837
Fat2
Name: FAT atypical cadherin 2
Synonyms: Fath2, mKIAA0811, LOC245827, EMI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Cdh8
Name: cadherin 8
Synonyms: cad8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12564
HGNC: HGNC:1767
Homologene: 55604
Abcc12
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 12
Synonyms: 4930467B22Rik, MRP9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 244562
Homologene: 57211
Synpo2l
Name: synaptopodin 2-like
Synonyms: Chap, 1110054M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 68760
Homologene: 23499
Vmn2r85
Name: vomeronasal 2, receptor 85
Synonyms: EG623734
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 623734
Homologene: 129606
Mamdc2
Name: MAM domain containing 2
Synonyms: mamcan, 1200015L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 71738
VEGA: 19
Homologene: 17722
Megf8
Name: multiple EGF-like-domains 8
Synonyms: Egfl4, b2b1702Clo, m687Ddg, b2b288Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Olfr1280
Name: olfactory receptor 1280
Synonyms: MOR248-1, GA_x6K02T2Q125-72366920-72367837
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258910
Homologene: 121540
Tshz2
Name: teashirt zinc finger family member 2
Synonyms: Zfp218, Tsh2, Mtsh2, teashirt2, Sdccag33l, 2900073F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228911
Homologene: 35258
Kynu
Name: kynureninase
Synonyms: 4432411A05Rik, L-kynurenine hydrolase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 70789
HGNC: HGNC:6469
Homologene: 2925
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: Bph, brachyphalangy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Sun1
Name: Sad1 and UNC84 domain containing 1
Synonyms: 4632417G13Rik, Unc84a, 5730434D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 77053
Homologene: 11544
Eml6
Name: echinoderm microtubule associated protein like 6
Synonyms: 2900083P10Rik, C230094A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237711
Homologene: 104282
Macrod1
Name: MACRO domain containing 1
Synonyms: D930010J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 107227
VEGA: 19
Homologene: 8549
Nrg3
Name: neuregulin 3
Synonyms: ska
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 18183
HGNC: HGNC:7999
Homologene: 32051
Atp1b2
Name: ATPase, Na+/K+ transporting, beta 2 polypeptide
Synonyms: Atpb-2, Amog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11932
HGNC: HGNC:805
Homologene: 36075
Rad51ap2
Name: RAD51 associated protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 209550
VEGA: 12
Homologene: 85245
Nags
Name: N-acetylglutamate synthase
Synonyms: 1700120E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217214
Homologene: 77363
Klf11
Name: Kruppel-like factor 11
Synonyms: Tieg2b, Tieg2, Tieg3, D12Ertd427e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 194655
Homologene: 2668
Aldh18a1
Name: aldehyde dehydrogenase 18 family, member A1
Synonyms: 2810433K04Rik, Pycs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 56454
HGNC: HGNC:9722
Homologene: 2142
Olfr313
Name: olfactory receptor 313
Synonyms: GA_x6K02T2NKPP-590035-589109, MOR222-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 258529
Homologene: 77375
Fbxw13
Name: F-box and WD-40 domain protein 13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 211305
Homologene: 110776
Vmn2r101
Name: vomeronasal 2, receptor 101
Synonyms: EG627576
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627576
Homologene: 115024
Tmem40
Name: transmembrane protein 40
Synonyms: 9030407H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 94346
Homologene: 10124
Gsg1l
Name: GSG1-like
Synonyms: C230098I05Rik, G630023A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269994
Homologene: 78152
Tktl2
Name: transketolase-like 2
Synonyms: 4933401I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 74419
Homologene: 69456
Cabyr
Name: calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2)
Synonyms: CBP86, 4933421A18Rik, 1700016C01Rik, FSP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 71132
Homologene: 49299
Irx1
Name: Iroquois homeobox 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 16371
Homologene: 19065
Igdcc4
Name: immunoglobulin superfamily, DCC subclass, member 4
Synonyms: Nope, WI-16786, WI-18508, 9330155G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 56741
VEGA: 9
Homologene: 10570
Plekhf1
Name: pleckstrin homology domain containing, family F (with FYVE domain) member 1
Synonyms: 1810013P09Rik, LAPF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72287
Homologene: 11516
Olfr1198
Name: olfactory receptor 1198
Synonyms: MOR225-13, GA_x6K02T2Q125-50221692-50220766
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 404330
Homologene: 138634
Pde6d
Name: phosphodiesterase 6D, cGMP-specific, rod, delta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18582
HGNC: HGNC:8788
Homologene: 1954
Zfp263
Name: zinc finger protein 263
Synonyms: mFPM315, 1200014J04Rik, NT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 74120
Homologene: 4197
D330041H03Rik
Name: RIKEN cDNA D330041H03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 654822
Acot8
Name: acyl-CoA thioesterase 8
Synonyms: Pte1, PTE-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 170789
Homologene: 3991
Dipk1a
Name: divergent protein kinase domain 1A
Synonyms: 2900024C23Rik, Fam69a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 67266
Homologene: 12104
Olfr1313
Name: olfactory receptor 1313
Synonyms: MOR245-23, GA_x6K02T2Q125-73119859-73118924
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258257
Homologene: 106834
Zfp772
Name: zinc finger protein 772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 232855
Homologene: 128539
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 82,529,564 bp
  • A to G, chromosome 1 at 82,651,258 bp
  • T to C, chromosome 1 at 86,547,526 bp
  • T to C, chromosome 1 at 153,465,699 bp
  • A to G, chromosome 2 at 43,602,300 bp
  • G to A, chromosome 2 at 48,937,489 bp
  • T to C, chromosome 2 at 69,467,135 bp
  • A to G, chromosome 2 at 88,746,144 bp
  • G to T, chromosome 2 at 91,995,444 bp
  • A to G, chromosome 2 at 111,316,116 bp
  • C to A, chromosome 2 at 112,071,975 bp
  • G to A, chromosome 2 at 164,804,684 bp
  • T to A, chromosome 2 at 169,883,951 bp
  • T to C, chromosome 2 at 180,174,301 bp
  • A to G, chromosome 3 at 87,446,464 bp
  • A to G, chromosome 4 at 108,177,134 bp
  • T to A, chromosome 4 at 132,857,361 bp
  • A to T, chromosome 5 at 86,037,617 bp
  • A to T, chromosome 5 at 99,223,045 bp
  • G to T, chromosome 5 at 107,914,424 bp
  • T to C, chromosome 5 at 139,235,601 bp
  • G to A, chromosome 5 at 145,221,079 bp
  • C to T, chromosome 6 at 30,374,764 bp
  • T to C, chromosome 6 at 115,741,615 bp
  • T to C, chromosome 7 at 7,203,700 bp
  • T to A, chromosome 7 at 25,349,019 bp
  • T to A, chromosome 7 at 27,818,162 bp
  • T to C, chromosome 7 at 38,221,577 bp
  • G to A, chromosome 7 at 80,901,242 bp
  • C to T, chromosome 7 at 125,891,680 bp
  • A to T, chromosome 8 at 33,574,885 bp
  • T to A, chromosome 8 at 66,513,206 bp
  • T to A, chromosome 8 at 86,543,907 bp
  • T to C, chromosome 8 at 99,196,386 bp
  • T to C, chromosome 8 at 108,956,032 bp
  • A to G, chromosome 9 at 20,500,944 bp
  • T to C, chromosome 9 at 65,132,058 bp
  • T to A, chromosome 9 at 72,972,406 bp
  • C to A, chromosome 9 at 109,184,231 bp
  • C to A, chromosome 9 at 119,462,452 bp
  • G to A, chromosome 10 at 4,090,007 bp
  • A to T, chromosome 10 at 8,742,422 bp
  • T to C, chromosome 10 at 128,488,159 bp
  • T to C, chromosome 10 at 130,425,212 bp
  • T to C, chromosome 11 at 29,809,332 bp
  • C to T, chromosome 11 at 46,756,225 bp
  • C to T, chromosome 11 at 55,252,171 bp
  • T to C, chromosome 11 at 58,817,727 bp
  • T to C, chromosome 11 at 69,602,879 bp
  • T to A, chromosome 11 at 102,145,984 bp
  • C to A, chromosome 12 at 11,456,757 bp
  • T to C, chromosome 12 at 24,655,491 bp
  • T to C, chromosome 13 at 15,660,941 bp
  • G to T, chromosome 13 at 71,963,292 bp
  • T to C, chromosome 14 at 20,662,180 bp
  • C to A, chromosome 14 at 38,370,949 bp
  • G to A, chromosome 16 at 3,749,716 bp
  • A to G, chromosome 16 at 73,002,010 bp
  • T to A, chromosome 17 at 19,588,815 bp
  • C to T, chromosome 17 at 24,412,419 bp
  • A to T, chromosome 17 at 85,013,860 bp
  • T to C, chromosome 18 at 12,750,966 bp
  • T to C, chromosome 18 at 20,977,730 bp
  • G to T, chromosome 19 at 7,196,494 bp
  • G to A, chromosome 19 at 23,310,932 bp
  • T to C, chromosome 19 at 40,574,369 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3085 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040574-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.