Strain Name:
C57BL/6J-MtgxR3409Btlr/Mmmh
Stock Number:
040627-MU
Citation ID:
RRID:MMRRC_040627-MU
Other Names:
R3409 (G1), C57BL/6J-MtgxR3409Btlr
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20495
Homologene: 286
Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17776
Homologene: 7428
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22222
Homologene: 7582
Klhl7
Name: kelch-like 7
Synonyms: 2700038B03Rik, SBBI26, Klhl6, D5Ertd363e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52323
Homologene: 10317
Snw1
Name: SNW domain containing 1
Synonyms: SKIP, NCoA-62, SNW1, 2310008B08Rik, Skiip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66354
VEGA: 12
Homologene: 56557
Commd9
Name: COMM domain containing 9
Synonyms: 1810029F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76501
Homologene: 8584
Smu1
Name: smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
Synonyms: 2600001O03Rik, SMU-1, 2610203K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74255
Homologene: 10079
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: l(7)-3Rn, Ten-m4, Doc4, l7Rn3, ELM2, Odz4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23966
Homologene: 8034
Krt10
Name: keratin 10
Synonyms: K10, K1C1, suprabasal cytokeratin 10, Krt-1.10, keratin 10, cytokeratin 10, D130054E02Rik, Krt1-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16661
HGNC: HGNC:6413
Homologene: 137236
Sptb
Name: spectrin beta, erythrocytic
Synonyms: Spnb-1, spectrin R, D330027P03Rik, LOC383567, brain erythroid spectrin (235E), Spnb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20741
Homologene: 295
Adgra3
Name: adhesion G protein-coupled receptor A3
Synonyms: 3830613O22Rik, Tem5-like, Gpr125
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70693
Homologene: 19235
Rpn2
Name: ribophorin II
Synonyms: Rpn-2, 1300012C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20014
Homologene: 2214
Dnpep
Name: aspartyl aminopeptidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13437
HGNC: HGNC:2981
Homologene: 6110
Ccdc88a
Name: coiled coil domain containing 88A
Synonyms: C330012F17Rik, D130005J21Rik, C130096N06Rik, GIV, HkRP1, Girdin, A430106J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108686
Homologene: 41180
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh-4, C130041O22Rik, Zfh4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80892
Homologene: 23477
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Cpsf1
Name: cleavage and polyadenylation specific factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 94230
VEGA: 15
HGNC: HGNC:2324
Homologene: 40865
H2-M10.6
Name: histocompatibility 2, M region locus 10.6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 399549
Homologene: 117973
Ccdc150
Name: coiled-coil domain containing 150
Synonyms: 4930511H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78016
Homologene: 15814
Frmd4a
Name: FERM domain containing 4A
Synonyms: C230040M21Rik, 2700017I06Rik, Gm13190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 209630
Homologene: 9971
Pitrm1
Name: pitrilysin metallepetidase 1
Synonyms: MP-1, 2310012C15Rik, Ntup1, PreP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 69617
VEGA: 13
Homologene: 5742
Atp13a4
Name: ATPase type 13A4
Synonyms: 4631413J11Rik, 9330174J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224079
Homologene: 75330
Nod1
Name: nucleotide-binding oligomerization domain containing 1
Synonyms: F830007N14Rik, Card4, Nlrc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 107607
Homologene: 4440
Serpina3n
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3N
Synonyms: Spi2-2, Spi2.2, Spi2/eb.4, antitrypsin, alpha-1 antiproteinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20716
HGNC: HGNC:16
Homologene: 111129
Or5b101
Name: olfactory receptor family 5 subfamily B member 101
Synonyms: GA_x6K02T2RE5P-3357666-3356743, MOR202-6, Olfr1453
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258695
HGNC: HGNC:8324
Homologene: 120159
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Nrxn1
Name: neurexin I
Synonyms: alpha-latrotoxin receptor (calcium-dependent), neurexin I beta, neurexin I alpha, neurexin I beta, neurexin I beta, neurexin I alpha, neurexin I alpha, 1700062G21Rik, A230068P09Rik, 9330127H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18189
HGNC: HGNC:8008
Homologene: 21005
Or5w8
Name: olfactory receptor family 5 subfamily W member 8
Synonyms: GA_x6K02T2Q125-49358694-49359620, MOR177-9, Olfr1151
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258631
Homologene: 36999
Vmn2r90
Name: vomeronasal 2, receptor 90
Synonyms: EG626942
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 626942
Aox4
Name: aldehyde oxidase 4
Synonyms: AOH2, 2310003G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71872
Homologene: 70273
Arhgap15
Name: Rho GTPase activating protein 15
Synonyms: 5830480G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76117
Homologene: 41271
H2-M2
Name: histocompatibility 2, M region locus 2
Synonyms: H-2M2, Thy19.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14990
Homologene: 133732
Scaf11
Name: SR-related CTD-associated factor 11
Synonyms: 1110061H03Rik, SRRP129, CASP11, SIP1, 2610510E10Rik, Sfrs2ip, Srsf2ip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72193
VEGA: 15
Homologene: 37957
Bank1
Name: B cell scaffold protein with ankyrin repeats 1
Synonyms: A530094C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242248
Homologene: 9926
Ripk3
Name: receptor-interacting serine-threonine kinase 3
Synonyms: Rip3, 2610528K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56532
VEGA: 14
Homologene: 31410
Sec14l5
Name: SEC14-like lipid binding 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 665119
VEGA: 16
Homologene: 96163
Lrrn4
Name: leucine rich repeat neuronal 4
Synonyms: B430119L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320974
Homologene: 17632
Tmem67
Name: transmembrane protein 67
Synonyms: 5330408M12Rik, b2b1291.1Clo, b2b1163.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329795
Homologene: 71886
Or10g1
Name: olfactory receptor family 10 subfamily G member 1
Synonyms: GA_x6K02T2RJGY-583652-584608, MOR223-6, Olfr1510
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258423
HGNC: HGNC:8170
Homologene: 87788
Nudcd1
Name: NudC domain containing 1
Synonyms: 4921532K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67429
VEGA: 15
Homologene: 12128
Or8c9
Name: olfactory receptor family 8 subfamily C member 9
Synonyms: MTPCR18, MOR170-4, GA_x6K02T2PVTD-32017922-32018863, Olfr25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18323
VEGA: 9
Ccdc185
Name: coiled-coil domain containing 185
Synonyms: 4922505E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 433386
Homologene: 17631
Pcdhb6
Name: protocadherin beta 6
Synonyms: Pcdhb5B, PcdhbF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93877
HGNC: HGNC:8690
Homologene: 62177
Slc26a9
Name: solute carrier family 26, member 9
Synonyms: anion transporter/exchanger-9, E030002L01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320718
Homologene: 14179
Or4c115
Name: olfactory receptor family 4 subfamily C member 115
Synonyms: GA_x6K02T2Q125-50579531-50578596, MOR233-5, Olfr1220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258902
Homologene: 74242
Mfsd9
Name: major facilitator superfamily domain containing 9
Synonyms: 4931419K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 211798
Homologene: 13100
Gsdmc4
Name: gasdermin C4
Synonyms: 9030605I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74548
HGNC: HGNC:7151
Homologene: 69487
Or4c1
Name: olfactory receptor family 4 subfamily C member 1
Synonyms: GA_x6K02T2Q125-50748233-50747292, MOR235-2, Olfr1231
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258446
Homologene: 27168
AI429214
Name: expressed sequence AI429214
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 621080
Homologene: 82747
Tal2
Name: T cell acute lymphocytic leukemia 2
Synonyms: bHLHa19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21350
Homologene: 3954
Gipr
Name: gastric inhibitory polypeptide receptor
Synonyms: LOC381853, LOC232937, glucose-dependent insulinotropic polypeptide receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381853
HGNC: HGNC:4271
Homologene: 20081
Six4
Name: sine oculis-related homeobox 4
Synonyms: AREC3, TrexBF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20474
Homologene: 69089
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 40,786,225 bp
  • A to G, chromosome 1 at 54,356,773 bp
  • T to C, chromosome 1 at 58,239,106 bp
  • A to G, chromosome 1 at 75,316,626 bp
  • T to C, chromosome 1 at 131,763,944 bp
  • T to G, chromosome 1 at 182,748,748 bp
  • A to T, chromosome 2 at 4,153,028 bp
  • T to C, chromosome 2 at 44,322,790 bp
  • T to A, chromosome 2 at 87,857,661 bp
  • T to C, chromosome 2 at 89,097,324 bp
  • A to G, chromosome 2 at 89,303,373 bp
  • G to A, chromosome 2 at 101,886,331 bp
  • A to C, chromosome 2 at 120,963,448 bp
  • A to G, chromosome 2 at 125,154,151 bp
  • G to A, chromosome 2 at 125,412,665 bp
  • A to G, chromosome 2 at 132,879,861 bp
  • C to A, chromosome 2 at 157,290,652 bp
  • C to T, chromosome 3 at 5,403,358 bp
  • G to A, chromosome 3 at 136,247,773 bp
  • A to T, chromosome 4 at 12,073,952 bp
  • T to C, chromosome 4 at 40,752,008 bp
  • A to G, chromosome 4 at 53,785,843 bp
  • C to G, chromosome 4 at 116,310,910 bp
  • G to T, chromosome 5 at 24,138,321 bp
  • A to G, chromosome 5 at 50,001,930 bp
  • G to T, chromosome 6 at 54,944,917 bp
  • A to G, chromosome 7 at 19,159,794 bp
  • A to G, chromosome 7 at 96,895,160 bp
  • C to T, chromosome 8 at 36,993,917 bp
  • T to A, chromosome 9 at 38,330,344 bp
  • G to A, chromosome 9 at 109,106,613 bp
  • T to A, chromosome 11 at 29,486,006 bp
  • C to A, chromosome 11 at 99,387,261 bp
  • A to G, chromosome 12 at 73,112,883 bp
  • T to G, chromosome 12 at 76,610,815 bp
  • A to T, chromosome 12 at 87,464,724 bp
  • A to G, chromosome 12 at 104,411,277 bp
  • G to A, chromosome 13 at 6,578,481 bp
  • T to A, chromosome 14 at 52,410,361 bp
  • T to G, chromosome 14 at 55,788,241 bp
  • T to C, chromosome 15 at 44,420,811 bp
  • A to G, chromosome 15 at 63,892,046 bp
  • G to T, chromosome 15 at 76,601,781 bp
  • A to G, chromosome 15 at 96,414,864 bp
  • C to T, chromosome 16 at 5,165,654 bp
  • G to T, chromosome 16 at 29,413,749 bp
  • G to T, chromosome 17 at 17,733,376 bp
  • C to T, chromosome 17 at 32,150,702 bp
  • T to A, chromosome 17 at 36,814,001 bp
  • T to A, chromosome 17 at 37,481,362 bp
  • G to A, chromosome 17 at 90,208,367 bp
  • A to G, chromosome 18 at 37,335,892 bp
  • G to C, chromosome 19 at 13,028,047 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3409 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040627-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.