Strain Name:
C57BL/6J-MtgxR3409Btlr/Mmmh
Stock Number:
040627-MU
Citation ID:
RRID:MMRRC_040627-MU
Other Names:
R3409 (G1), C57BL/6J-MtgxR3409Btlr
Major Collection:

Gene Information

Gene Symbol: Slc12a1 [MGI:103150] (Mus musculus (mouse))
Name: solute carrier family 12, member 1
Synonyms: urehr3, Nkcc2, D630042G03Rik, mBSC1
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Notch3 [MGI:99460] (Mus musculus (mouse))
Name: notch 3
Synonyms: N3, hpbk
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Mast2 [MGI:894676] (Mus musculus (mouse))
Name: microtubule associated serine/threonine kinase 2
Synonyms: Mtssk, MAST205
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Ubr1 [MGI:1277977] (Mus musculus (mouse))
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Klhl7 [MGI:1196453] (Mus musculus (mouse))
Name: kelch-like 7
Synonyms: 2700038B03Rik, SBBI26, Klhl6, D5Ertd363e
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Snw1 [MGI:1913604] (Mus musculus (mouse))
Name: SNW domain containing 1
Synonyms: SNW1, Skiip, NCoA-62, SKIP, 2310008B08Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Commd9 [MGI:1923751] (Mus musculus (mouse))
Name: COMM domain containing 9
Synonyms: 1810029F08Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 40,786,225 bp
  • A to G, chromosome 1 at 54,356,773 bp
  • T to C, chromosome 1 at 58,239,106 bp
  • A to G, chromosome 1 at 75,316,626 bp
  • T to C, chromosome 1 at 131,763,944 bp
  • T to G, chromosome 1 at 182,748,748 bp
  • A to T, chromosome 2 at 4,153,028 bp
  • T to C, chromosome 2 at 44,322,790 bp
  • T to A, chromosome 2 at 87,857,661 bp
  • T to C, chromosome 2 at 89,097,324 bp
  • A to G, chromosome 2 at 89,303,373 bp
  • G to A, chromosome 2 at 101,886,331 bp
  • A to C, chromosome 2 at 120,963,448 bp
  • A to G, chromosome 2 at 125,154,151 bp
  • G to A, chromosome 2 at 125,412,665 bp
  • A to G, chromosome 2 at 132,879,861 bp
  • C to A, chromosome 2 at 157,290,652 bp
  • C to T, chromosome 3 at 5,403,358 bp
  • G to A, chromosome 3 at 136,247,773 bp
  • A to T, chromosome 4 at 12,073,952 bp
  • T to C, chromosome 4 at 40,752,008 bp
  • A to G, chromosome 4 at 53,785,843 bp
  • C to G, chromosome 4 at 116,310,910 bp
  • G to T, chromosome 5 at 24,138,321 bp
  • A to G, chromosome 5 at 50,001,930 bp
  • G to T, chromosome 6 at 54,944,917 bp
  • A to G, chromosome 7 at 19,159,794 bp
  • A to G, chromosome 7 at 96,895,160 bp
  • C to T, chromosome 8 at 36,993,917 bp
  • T to A, chromosome 9 at 38,330,344 bp
  • G to A, chromosome 9 at 109,106,613 bp
  • T to A, chromosome 11 at 29,486,006 bp
  • C to A, chromosome 11 at 99,387,261 bp
  • A to G, chromosome 12 at 73,112,883 bp
  • T to G, chromosome 12 at 76,610,815 bp
  • A to T, chromosome 12 at 87,464,724 bp
  • A to G, chromosome 12 at 104,411,277 bp
  • G to A, chromosome 13 at 6,578,481 bp
  • T to A, chromosome 14 at 52,410,361 bp
  • T to G, chromosome 14 at 55,788,241 bp
  • T to C, chromosome 15 at 44,420,811 bp
  • A to G, chromosome 15 at 63,892,046 bp
  • G to T, chromosome 15 at 76,601,781 bp
  • A to G, chromosome 15 at 96,414,864 bp
  • C to T, chromosome 16 at 5,165,654 bp
  • G to T, chromosome 16 at 29,413,749 bp
  • G to T, chromosome 17 at 17,733,376 bp
  • C to T, chromosome 17 at 32,150,702 bp
  • T to A, chromosome 17 at 36,814,001 bp
  • T to A, chromosome 17 at 37,481,362 bp
  • G to A, chromosome 17 at 90,208,367 bp
  • A to G, chromosome 18 at 37,335,892 bp
  • G to C, chromosome 19 at 13,028,047 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3409 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040627-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.