Strain Name:
C57BL/6J-MtgxR3500Btlr/Mmmh
Stock Number:
040663-MU
Citation ID:
RRID:MMRRC_040663-MU
Other Names:
R3500 (G1), C57BL/6J-MtgxR3500Btlr
Major Collection:

Gene Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Elavl3
Name: ELAV like RNA binding protein 3
Synonyms: mHuC, Huc, 2600009P04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15571
VEGA: 9
HGNC: HGNC:3314
Homologene: 31035
Arl15
Name: ADP-ribosylation factor-like 15
Synonyms: C230032K13Rik, Arfrp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218639
Homologene: 56843
Lactb2
Name: lactamase, beta 2
Synonyms: E430032H21Rik, Cgi-83
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212442
Homologene: 9349
Nbea
Name: neurobeachin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 26422
HGNC: HGNC:7648
Homologene: 69190
Cnot4
Name: CCR4-NOT transcription complex, subunit 4
Synonyms: Not4h, Not4, Not4hp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 53621
HGNC: HGNC:7880
Homologene: 40870
Nedd4l
Name: neural precursor cell expressed, developmentally down-regulated gene 4-like
Synonyms: 1300012C07Rik, Nedd4-2, Nedd4b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 83814
VEGA: 18
HGNC: HGNC:7728
Homologene: 86986
Eftud2
Name: elongation factor Tu GTP binding domain containing 2
Synonyms: U5-116kD, 116kDa, Snrp116
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20624
Homologene: 3133
Plcg2
Name: phospholipase C, gamma 2
Synonyms: Plcg-2, PLCgamma2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234779
HGNC: HGNC:9066
Homologene: 55671
Map3k11
Name: mitogen-activated protein kinase kinase kinase 11
Synonyms: Mlk3, 2610017K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 26403
VEGA: 19
HGNC: HGNC:6850
Homologene: 37618
Amhr2
Name: anti-Mullerian hormone type 2 receptor
Synonyms: MIS TypeII receptor, MISIIR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110542
HGNC: HGNC:465
Homologene: 10746
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Nr5a1
Name: nuclear receptor subfamily 5, group A, member 1
Synonyms: Ad4BP, SF1, SF-1, Ftz-F1, steroidogenic factor 1, adrenal 4-binding protein, ELP, Ftzf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26423
HGNC: HGNC:7983
Homologene: 3638
Podxl2
Name: podocalyxin-like 2
Synonyms: D130074J02Rik, PODLX2, Endoglycan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 319655
Homologene: 9254
Mob1b
Name: MOB kinase activator 1B
Synonyms: 1110003E08Rik, Mobkl1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68473
Homologene: 61676
Spg11
Name: SPG11, spatacsin vesicle trafficking associated
Synonyms: C530005A01Rik, 6030465E24Rik, spastic paraplegia 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 214585
Homologene: 41614
Ppp3ca
Name: protein phosphatase 3, catalytic subunit, alpha isoform
Synonyms: CnA, PP2B alpha 1, PP2BA alpha, Caln, Calna, 2900074D19Rik, CN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19055
HGNC: HGNC:9314
Homologene: 55497
Copg1
Name: coatomer protein complex, subunit gamma 1
Synonyms: D6Ertd71e, D6Wsu16e, Copg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54161
HGNC: HGNC:2236
Homologene: 56745
Prdm10
Name: PR domain containing 10
Synonyms: tristanin, LOC382066
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382066
Homologene: 45718
Trpm2
Name: transient receptor potential cation channel, subfamily M, member 2
Synonyms: Trrp7, LTRPC2, TRPC7, 9830168K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 28240
Homologene: 20709
Gata4
Name: GATA binding protein 4
Synonyms: Gata-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14463
HGNC: HGNC:4173
Homologene: 1551
Mecom
Name: MDS1 and EVI1 complex locus
Synonyms: ZNFPR1B1, Prdm3, MDS1-EVI1, Evi-1, D630039M04Rik, Jbo, Evi1, Mds1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14013
HGNC: HGNC:3498
Homologene: 21086
Fgd2
Name: FYVE, RhoGEF and PH domain containing 2
Synonyms: tcs-2, Tcd-2, tcs2, Tcd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 26382
HGNC: HGNC:3664
Homologene: 8438
Aldh1a1
Name: aldehyde dehydrogenase family 1, subfamily A1
Synonyms: ALDH1, E1, Ahd-2, Ahd2, Raldh1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 11668
HGNC: HGNC:402
Homologene: 110441
Soat2
Name: sterol O-acyltransferase 2
Synonyms: ACAT2, D15Wsu97e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223920
Homologene: 68355
Clcn1
Name: chloride channel, voltage-sensitive 1
Synonyms: SMCC1, Clc-1, Clc1, NMF355, nmf355
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12723
HGNC: HGNC:2019
Homologene: 63
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Rab44
Name: RAB44, member RAS oncogene family
Synonyms: 9830134C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 442827
Homologene: 53300
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Clstn3
Name: calsyntenin 3
Synonyms: Cst-3, CSTN3, alcadein-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232370
Homologene: 22836
Olfr1224-ps1
Name: olfactory receptor 1224, pseudogene 1
Synonyms: GA_x6K02T2Q125-50635980-50635046, MOR233-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258026
Olfr1189
Name: olfactory receptor 1189
Synonyms: GA_x6K02T2Q125-50079044-50079964, MOR237-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258768
Homologene: 81569
Grid2
Name: glutamate receptor, ionotropic, delta 2
Synonyms: GluRdelta2, tpr, B230104L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14804
HGNC: HGNC:4576
Homologene: 74399
Gabrr1
Name: gamma-aminobutyric acid (GABA) C receptor, subunit rho 1
Synonyms: GABA-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14408
HGNC: HGNC:4090
Homologene: 20470
Pcdhb19
Name: protocadherin beta 19
Synonyms: Pcdhb11, PcdhbS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93890
HGNC: HGNC:8683
Homologene: 128411
Sdk1
Name: sidekick cell adhesion molecule 1
Synonyms: 6720466O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330222
Homologene: 27395
Spib
Name: Spi-B transcription factor (Spi-1/PU.1 related)
Synonyms: Spi-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 272382
Homologene: 79220
Rhbdl3
Name: rhomboid like 3
Synonyms: Ventrhoid, Vrho, Rhbdl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 246104
Homologene: 34700
Chil5
Name: chitinase-like 5
Synonyms: Bclp2, Bclp1, Chi3l7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229687
Homologene: 132668
Hdac9
Name: histone deacetylase 9
Synonyms: HDRP, Hdac7b, Mitr, D030072B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 79221
Homologene: 128578
Tas2r122
Name: taste receptor, type 2, member 122
Synonyms: Tas2r22, mGR22, T2R22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 630845
Homologene: 105455
Tbpl2
Name: TATA box binding protein like 2
Synonyms: LOC227606, Trf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227606
Homologene: 133152
Ldhb
Name: lactate dehydrogenase B
Synonyms: lactate dehydrogenase-B, Ldh-2, H-Ldh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16832
HGNC: HGNC:6541
Homologene: 55647
Vmn2r23
Name: vomeronasal 2, receptor 23
Synonyms: EG435916
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 435916
Homologene: 135826
Olfr1426
Name: olfactory receptor 1426
Synonyms: GA_x6K02T2RE5P-2447610-2446675, MOR239-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258805
Homologene: 128079
Czib
Name: CXXC motif containing zinc binding protein
Synonyms: 0610037L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74098
Homologene: 41206
Olfr1317
Name: olfactory receptor 1317
Synonyms: GA_x6K02T2Q125-73188162-73189112, MOR245-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258440
Homologene: 71983
Pramel6
Name: PRAME like 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 347711
Homologene: 77635
Cetn4
Name: centrin 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 207175
Homologene: 138302
Olfr589
Name: olfactory receptor 589
Synonyms: GA_x6K02T2PBJ9-5871256-5870303, MOR32-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259054
Homologene: 133046
Gm9396
Name: predicted gene 9396
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 668844
Prr19
Name: proline rich 19
Synonyms: EG623131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 623131
Homologene: 85197
Fancb
Name: Fanconi anemia, complementation group B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 237211
HGNC: HGNC:3583
Homologene: 51880
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 13,660,449 bp
  • CTCTCTCTCTCTCTCTCTCTCTCTCTC to CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC, chromosome 1 at 88,247,714 bp
  • C to T, chromosome 2 at 24,087,139 bp
  • G to A, chromosome 2 at 38,707,940 bp
  • T to C, chromosome 2 at 52,325,785 bp
  • G to A, chromosome 2 at 76,730,284 bp
  • G to T, chromosome 2 at 76,761,165 bp
  • A to G, chromosome 2 at 87,509,225 bp
  • A to G, chromosome 2 at 88,591,941 bp
  • C to T, chromosome 2 at 89,157,059 bp
  • T to C, chromosome 2 at 112,142,127 bp
  • A to G, chromosome 2 at 122,055,529 bp
  • C to T, chromosome 3 at 29,980,912 bp
  • A to T, chromosome 3 at 37,309,960 bp
  • A to G, chromosome 3 at 55,681,010 bp
  • T to C, chromosome 3 at 106,018,220 bp
  • C to A, chromosome 3 at 130,068,495 bp
  • A to G, chromosome 3 at 136,881,512 bp
  • T to C, chromosome 4 at 33,158,184 bp
  • C to T, chromosome 4 at 107,891,513 bp
  • A to T, chromosome 5 at 25,299,479 bp
  • A to G, chromosome 5 at 88,749,620 bp
  • G to T, chromosome 5 at 142,006,616 bp
  • G to A, chromosome 6 at 35,080,141 bp
  • T to C, chromosome 6 at 42,292,995 bp
  • T to C, chromosome 6 at 63,503,399 bp
  • G to A, chromosome 6 at 87,895,923 bp
  • T to C, chromosome 6 at 88,842,918 bp
  • T to C, chromosome 6 at 123,713,170 bp
  • A to T, chromosome 6 at 124,431,711 bp
  • T to A, chromosome 6 at 132,711,560 bp
  • T to C, chromosome 6 at 142,501,447 bp
  • A to G, chromosome 7 at 25,303,267 bp
  • T to C, chromosome 7 at 44,528,823 bp
  • T to C, chromosome 7 at 103,155,090 bp
  • A to G, chromosome 8 at 117,612,978 bp
  • A to G, chromosome 9 at 22,018,744 bp
  • T to A, chromosome 9 at 31,359,166 bp
  • A to G, chromosome 10 at 77,932,302 bp
  • A to G, chromosome 11 at 55,260,516 bp
  • T to C, chromosome 11 at 80,319,705 bp
  • A to G, chromosome 11 at 102,844,180 bp
  • C to A, chromosome 12 at 34,437,353 bp
  • G to A, chromosome 13 at 113,967,692 bp
  • G to T, chromosome 14 at 52,205,653 bp
  • C to T, chromosome 14 at 63,200,533 bp
  • C to T, chromosome 15 at 102,161,163 bp
  • A to G, chromosome 15 at 102,447,066 bp
  • C to T, chromosome 17 at 29,138,067 bp
  • T to C, chromosome 17 at 29,365,601 bp
  • T to A, chromosome 18 at 37,497,479 bp
  • G to A, chromosome 18 at 65,212,860 bp
  • A to T, chromosome 19 at 5,690,247 bp
  • A to G, chromosome 19 at 12,088,057 bp
  • T to C, chromosome 19 at 20,610,902 bp
  • A to G, chromosome X at 164,996,108 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3500 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040663-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.