Strain Name:
C57BL/6J-MtgxR4303Btlr/Mmmh
Stock Number:
041090-MU
Citation ID:
RRID:MMRRC_041090-MU
Other Names:
R4303 (G1), C57BL/6J-MtgxR4303Btlr
Major Collection:

Gene Information

Ypel4
Name: yippee like 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241525
Homologene: 128276
Heatr5a
Name: HEAT repeat containing 5A
Synonyms: D930036F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320487
VEGA: 12
Homologene: 19635
Chaf1a
Name: chromatin assembly factor 1, subunit A (p150)
Synonyms: CAF-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 27221
VEGA: 17
HGNC: HGNC:1910
Homologene: 4003
Rsf1
Name: remodeling and spacing factor 1
Synonyms: 4832420A03Rik, XAP8, Hbxap, C030033M12Rik, p325
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Map3k11
Name: mitogen-activated protein kinase kinase kinase 11
Synonyms: 2610017K16Rik, Mlk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 26403
VEGA: 19
HGNC: HGNC:6850
Homologene: 37618
Abcb8
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 8
Synonyms: 4833412N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74610
HGNC: HGNC:49
Homologene: 5203
Ift80
Name: intraflagellar transport 80
Synonyms: 4921524P20Rik, Wdr56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68259
Homologene: 12253
Ehmt2
Name: euchromatic histone lysine N-methyltransferase 2
Synonyms: D17Ertd710e, NG36, G9a, Bat8, KMT1C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110147
Homologene: 48460
Hoxa5
Name: homeobox A5
Synonyms: Hox-1.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15402
HGNC: HGNC:5106
Homologene: 40726
Pigr
Name: polymeric immunoglobulin receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18703
HGNC: HGNC:8968
Homologene: 1984
Tax1bp1
Name: Tax1 (human T cell leukemia virus type I) binding protein 1
Synonyms: D6Ertd772e, T6BP, 1700069J21Rik, TXBP151, 1200003J11Rik, D6Ertd404e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 52440
Homologene: 4395
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: Hapip, E530005C20Rik, LOC224126, 2210407G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Trim29
Name: tripartite motif-containing 29
Synonyms: 2810431N19Rik, 1110047J21Rik, 4732461M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72169
VEGA: 9
Homologene: 8100
Ern2
Name: endoplasmic reticulum (ER) to nucleus signalling 2
Synonyms: Ire1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26918
Homologene: 22687
Sox6
Name: SRY (sex determining region Y)-box 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20679
Homologene: 22631
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: Spna-1, erythroid, Spna1, ihj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20739
Homologene: 74460
Borcs8
Name: BLOC-1 related complex subunit 8
Synonyms: 2310045N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72368
Homologene: 47492
Wdr31
Name: WD repeat domain 31
Synonyms: 5430402I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 71354
Homologene: 11360
Olfr293
Name: olfactory receptor 293
Synonyms: GA_x6K02T2NHDJ-9457744-9456734, MOR221-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 257906
Homologene: 79385
Atg4b
Name: autophagy related 4B, cysteine peptidase
Synonyms: Apg4b, 2510009N07Rik, autophagin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66615
Homologene: 100868
Slco3a1
Name: solute carrier organic anion transporter family, member 3a1
Synonyms: 5830414C08Rik, MJAM, Anr1, Slc21a11, OATP-D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108116
Homologene: 40862
Serpina1a
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1A
Synonyms: Aat2, Spi1-1, Aat-2, PI1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20700
HGNC: HGNC:8941
Homologene: 20103
Olfr389
Name: olfactory receptor 389
Synonyms: MOR135-6, GA_x6K02T2P1NL-3932085-3931147
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259011
Homologene: 85925
Pik3ca
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Synonyms: p110alpha, 6330412C24Rik, caPI3K
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18706
HGNC: HGNC:8975
Homologene: 21249
Serpina3i
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3I
Synonyms: Gm6930, 2B2, antitrypsin, alpha-1 antiproteinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 628900
HGNC: HGNC:16
Homologene: 115927
Shank1
Name: SH3 and multiple ankyrin repeat domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243961
Homologene: 22949
Nuggc
Name: nuclear GTPase, germinal center associated
Synonyms: Gm600, LOC239151, SLIP-GC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100503545
Homologene: 72641
Myo1a
Name: myosin IA
Synonyms: BBM-I, brush border myosin 1, Myhl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432516
VEGA: 10
HGNC: HGNC:7595
Homologene: 21113
Esp6
Name: exocrine gland secreted peptide 6
Synonyms: Gm5494, Esp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 433107
VEGA: 17
Cacna2d1
Name: calcium channel, voltage-dependent, alpha2/delta subunit 1
Synonyms: Cchl2a, Cacna2, Ca(v)alpha2delta1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12293
HGNC: HGNC:1399
Homologene: 579
Ehhadh
Name: enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Synonyms: MFP, MFP1, 1300002P22Rik, HD, L-bifunctional enzyme, L-PBE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74147
HGNC: HGNC:3247
Homologene: 1486
Defb12
Name: defensin beta 12
Synonyms: 9230103N16Rik, mBD-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 77674
Homologene: 17531
Fam189a2
Name: family with sequence similarity 189, member A2
Synonyms: LOC381217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381217
VEGA: 19
Homologene: 3540
Mrgpra4
Name: MAS-related GPR, member A4
Synonyms: MrgA4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 235854
Homologene: 131159
Vmn1r90
Name: vomeronasal 1 receptor 90
Synonyms: B430211C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 627280
Homologene: 122945
Zfp974
Name: zinc finger protein 974
Synonyms: 1700049G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73430
Krt36
Name: keratin 36
Synonyms: HRa-1, Krt1-22, keratin 5, Krt1-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16673
HGNC: HGNC:6454
Homologene: 88459
Gm17333
Name: predicted gene, 17333
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Zfp39
Name: zinc finger protein 39
Synonyms: Zfp-39, CTfin33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22698
Homologene: 69102
Stard13
Name: StAR-related lipid transfer (START) domain containing 13
Synonyms: GT650, DLC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243362
Homologene: 64844
Dnah7c
Name: dynein, axonemal, heavy chain 7C
Synonyms: Dnahc7c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100101919
Homologene: 41287
Zfp579
Name: zinc finger protein 579
Synonyms: 1110003A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68490
Homologene: 17629
Bckdk
Name: branched chain ketoacid dehydrogenase kinase
Synonyms: BCKD-kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12041
Homologene: 37642
Six4
Name: sine oculis-related homeobox 4
Synonyms: TrexBF, AREC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20474
Homologene: 69089
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 46,748,578 bp
  • A to G, chromosome 1 at 93,768,262 bp
  • G to A, chromosome 1 at 130,841,817 bp
  • A to G, chromosome 1 at 174,179,852 bp
  • A to G, chromosome 2 at 84,736,807 bp
  • C to T, chromosome 3 at 32,439,935 bp
  • A to G, chromosome 3 at 68,894,174 bp
  • T to A, chromosome 4 at 62,460,389 bp
  • A to G, chromosome 5 at 16,302,248 bp
  • T to C, chromosome 5 at 24,401,057 bp
  • T to C, chromosome 5 at 151,062,869 bp
  • T to C, chromosome 6 at 52,204,260 bp
  • T to C, chromosome 6 at 52,727,278 bp
  • A to G, chromosome 7 at 4,993,073 bp
  • C to T, chromosome 7 at 14,561,570 bp
  • C to A, chromosome 7 at 27,910,232 bp
  • A to G, chromosome 7 at 44,342,474 bp
  • T to A, chromosome 7 at 47,980,936 bp
  • G to T, chromosome 7 at 74,554,528 bp
  • G to A, chromosome 7 at 86,663,955 bp
  • CGGCGGCGG to CGGCGGCGGTGGCGGCGG, chromosome 7 at 97,579,920 bp
  • C to T, chromosome 7 at 115,544,469 bp
  • T to C, chromosome 7 at 122,177,846 bp
  • T to C, chromosome 7 at 127,905,330 bp
  • A to G, chromosome 8 at 19,112,721 bp
  • T to C, chromosome 8 at 70,136,212 bp
  • G to T, chromosome 9 at 43,311,122 bp
  • A to G, chromosome 10 at 127,713,733 bp
  • T to C, chromosome 11 at 58,890,017 bp
  • A to T, chromosome 11 at 73,776,838 bp
  • A to T, chromosome 11 at 100,103,413 bp
  • T to C, chromosome 12 at 51,956,225 bp
  • G to T, chromosome 12 at 73,112,540 bp
  • A to G, chromosome 12 at 103,854,675 bp
  • G to A, chromosome 12 at 104,265,330 bp
  • A to G, chromosome 14 at 65,611,172 bp
  • C to A, chromosome 16 at 21,762,852 bp
  • T to C, chromosome 16 at 34,235,391 bp
  • A to C, chromosome 16 at 77,852,879 bp
  • G to A, chromosome 17 at 34,908,748 bp
  • A to G, chromosome 17 at 40,565,144 bp
  • A to G, chromosome 17 at 56,044,068 bp
  • T to A, chromosome 19 at 5,690,824 bp
  • G to A, chromosome 19 at 23,975,629 bp
  • C to T, chromosome 19 at 23,975,638 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4303 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041090-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.